Adaptogenic effect of Morus alba on chronic footshock-induced stress in rats

Abstract

Objective:The objective of the present study was to evaluate the adaptogenic property of the ethyl acetate-soluble fraction of methanol extract of Morus alba roots against a rat model of chronic stress (CS).Materials and Methods:Rats were exposed to stress procedure for 21 days. The stress procedure was mild, unpredictable footshock, administered for 1 h once daily for 21 days. Rats were administered with the ethyl acetate soluble fraction of methanol extract of M. alba roots (25, 50 and 100 mg/kg p.o) 1 h before footshock for 21 days and behavioral parameters were evaluated for cognitive dysfunction and depression using elevated plus maze and despair swim test, respectively. On day 21, rats were sacrificed immediately after stress and blood was collected for biochemical estimation. The adrenal gland and spleen were dissected for organ weight and the stomach was dissected for ulcer score.Results:CS significantly induced cognitive deficit, mental depression and hyperglycemia and increased blood corticosterone levels, gastric ulcerations and adrenal gland weight, but decreased the splenic weight. Pre-treatments with the ethyl acetate soluble fraction of methanol extract of M. alba roots (25, 50 and 100 mg/kg, p.o.) significantly attenuated the CS-induced perturbations. Diazepam (1 mg/kg, p.o.) was used as the standard antistress drug.Conclusion:The results indicate that M. alba possesses significant adaptogenic activity, indicating its possible clinical utility as an antistress agent.

Glucometabolic effects of single and repeated exposure to forced-swimming stressor in Sprague-Dawley rats

“…Indeed, recent evidence links the stress-induced increases in glucocorticoids with dysregulation of glucose control (Gregoire et al 1991;Ahn et al 2016). Corticosterone mobilizes stored energy reserves in body tissues, leading to an elevated blood glucose levels (Nade et al 2009). Our lab has also previously shown that the underlying mechanism by which physical stress disrupt glucose control may involve blunting of insulin signaling in skeletal muscles (Morakinyo et al 2016).…”

A practical strategy for chemical profiling of herbal medicines using ultra-high performance liquid chromatography coupled with hybrid triple quadrupole-linear ion trap mass spectrometry: a case study of Mori Cortex

“…Under negative mode, neutral cleavages of H 2 O and CO 2 groups are the characteristic behaviors of stilbenes, and another neutral loss of a glycosyl group could be detected for stilbene glycoside. As the di-glycosidation product of oxyresveratrol, mulberroside A (3) was detected at the retention time (t R ) of 8.7 min by comparison with the reference compound, while oxyresveratrol (16) was observed at 14.7 min (Table 1 and Fig. 9).…”

UPLC-DAD-QTOF/MS Analysis of Flavonoids from 12 Varieties of Korean Mulberry Fruit

“…Mulberry belongs to the Moraceae family, which includes 37 genera and over 1100 species [1] and has long been used as a traditional medicine and food in Korea, China, and Japan [2][3][4]. Mulberries are reported to exhibit numerous pharmacological activities, such as antidiabetic [5], antioxidant [6], anticancer [7], antistress [8], immunomodulatory [9], and hepatoprotective [10] activities. erefore, mulberries should be considered as important potential sources of bioactive compounds that can be used for the prevention or treatment of many di erent medical conditions.…”