Adaptively controlled supercontinuum pulse from a microstructure fiber for two-photon excited fluorescence microscopy

Tada, Junji; Kôno, Tatsuo; Suda, Akira; Mizuno, Hideaki; Miyawaki, Atsushi; Midorikawa, Katsumi; Kannari, Fumihiko

doi:10.1364/ao.46.003023

Cited by 22 publications

(17 citation statements)

References 22 publications

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“…The combination of an SC source and pulse shaping can offer control of two-photon excitation over an ultrabroad spectral range. Adaptive pulse shaping of the excitation source to achieve selective flurophore excitation has been demonstrated [25]. However, pulse shaping of SC for TPF, SHG, and THG imaging of biological samples has not been shown.…”

Section: Introductionmentioning

confidence: 99%

Multimodal Nonlinear Microscopy by Shaping a Fiber Supercontinuum From 900 to 1160 nm

Liu

Benalcazar

et al. 2012

IEEE J. Select. Topics Quantum Electron.

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Nonlinear microscopy has become widely used in biophotonic imaging. Pulse shaping provides control over nonlinear optical processes of ultrafast pulses for selective imaging and contrast enhancement. In this study, nonlinear microscopy, including two-photon fluorescence, second harmonic generation, and third harmonic generation, was performed using pulses shaped from a fiber supercontinuum (SC) spanning from 900 to 1160 nm. The SC generated by coupling pulses from a Yb:KYW pulsed laser into a photonic crystal fiber was spectrally filtered and compressed using a spatial light modulator. The shaped pulses were used for nonlinear optical imaging of cellular and tissue samples. Amplitude and phase shaping the fiber SC offers selective and efficient nonlinear optical imaging over a broad bandwidth with a single-beam and an easily tunable setup.

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Section: Introductionmentioning

confidence: 99%

Multimodal Nonlinear Microscopy by Shaping a Fiber Supercontinuum From 900 to 1160 nm

Liu

Benalcazar

et al. 2012

IEEE J. Select. Topics Quantum Electron.

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“…Supercontinuum lasers have recently been used in other biomedical fluorescence techniques, including Raman scattering spectroscopy, confocal and two-photon microscopy (20, 21, 22, 23). However, the application of this laser technology to flow cytometry has been limited.…”

Section: Discussionmentioning

confidence: 99%

Supercontinuum white light lasers for flow cytometry

2008

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Excitation of fluorescent probes for flow cytometry has traditionally been limited to a few discrete laser lines, an inherent limitation in our ability to excite the vast array of fluorescent probes available for cellular analysis. In this report, we have used a supercontinuum (SC) white light laser as an excitation source for flow cytometry. By selectively filtering the wavelength of interest, almost any laser wavelength in the visible spectrum can be separated and used for flow cytometric analysis. The white light lasers used in this study were integrated into a commercial flow cytometry platform, and a series of high-transmission bandpass filters used to select wavelength ranges from the blue ($480 nm) to the long red ([700 nm). Cells labeled with a variety of fluorescent probes or expressing fluorescent proteins were then analyzed, in comparison with traditional lasers emitting at wavelengths similar to the filtered SC source. Based on a standard sensitivity metric, the white light laser bandwidths produced similar excitation levels to traditional lasers for a wide variety of fluorescent probes and expressible proteins. Sensitivity assessment using fluorescent bead arrays confirmed that the SC laser and traditional sources resulted in similar levels of detection sensitivity. Supercontinuum white light laser sources therefore have the potential to remove a significant barrier in flow cytometric analysis, namely the limitation of excitation wavelengths. Almost any visible wavelength range can be made available for excitation, allowing access to virtually any fluorescent probe, and permitting ''fine-tuning'' of excitation wavelength to particular probes. ' International Society for Advancement of CytometryKey terms flow cytometry; supercontinuum; white light laser; immunophenotyping; fluorescent protein; DsRed; mCherry; Katushka FLOW cytometry relies almost exclusively on lasers as a source of excitation for fluorescent probes. Although the coherence and power level makes them ideal sources for illuminating individual cells, their discrete wavelengths limits the range of excitation bandwidths that are available for fluorescent probe excitation. Even the most modern multi-laser flow cytometers typically provide no more than six-laser wavelengths, allowing excitation of only a fraction of the vast range of fluorescent probes available for biomedical analysis. Single wavelength lasers in formats suitable for flow cytometry have traditionally been limited; for many years, instrumentation was typically limited to 488 nm and red laser sources, with other wavelengths being less common and available only on instrumentation, which could accommodate large-frame ion lasers (1). More recent laser diode technology has provided UV and violet sources applicable for cytometry, and diode pumped solid state (DPSS) laser technology has provided blue, green, and more recently yellow and orange lasers that can be integrated into cytometric instrumentation (2-6). These new laser sources have greatly expanded our access to ...

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“…We also mention numerical works in which genetic algorithms (GAs) were applied to determine the carrier wavelength, power and duration of the pump pulse to control the frequency location of solitonic components of the SC spectrum [18,19]. Whereas previous successful experiments [14,15] focused on gaining control over limited SC spectral range, in this study we experimentally demonstrate enhancement and shaping of pre-chosen spectral features over the full SC spectrum (excluding the spectral range of the pump pulse). We also quantify and measure the temporal stability of the SC spectrum which is an important factor that can influence the convergence of the GA.…”

Section: Introductionmentioning

confidence: 86%

Genetic algorithm driven spectral shaping of supercontinuum radiation in a photonic crystal fiber

Michaeli

Bahabad

2018

J. Opt.

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Adaptively controlled supercontinuum pulse from a microstructure fiber for two-photon excited fluorescence microscopy

Cited by 22 publications

References 22 publications

Multimodal Nonlinear Microscopy by Shaping a Fiber Supercontinuum From 900 to 1160 nm

Multimodal Nonlinear Microscopy by Shaping a Fiber Supercontinuum From 900 to 1160 nm

Supercontinuum white light lasers for flow cytometry

Genetic algorithm driven spectral shaping of supercontinuum radiation in a photonic crystal fiber

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