Adaptive sex-dependent changes in the zonation of carbohydrate and lipid metabolism in rat liver lobules after partial hepatectomy

Noorden, C. J. F. Van; Vogels, I. M. C.; James, J.

doi:10.1016/0270-9139(94)90109-0

Cited by 4 publications

(2 citation statements)

References 66 publications

(52 reference statements)

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“…In this study, the upregulation of FAT/CD36 and hepatic fat accumulation were found in female rat livers after PHX. Multiple studies have reported that hepatic fat accumulation occurs from 24 to 48 hr after 70% PHX in rats [17, 21, 36], which is concomitant with hepatocellular proliferation [10, 39]. Our results revealed that female rat livers exhibit marked fat accumulation in regenerating liver that started from 6 hr and peaked at 24 hr after PHX, whereas fat accumulation in male rat livers started from 36 hr after PHX.…”

Section: Discussionsupporting

confidence: 49%

The Effect of Estrogen on Hepatic Fat Accumulation during Early Phase of Liver Regeneration after Partial Hepatectomy in Rats

Srisowanna

Choijookhuu

Yano

et al. 2019

Acta Histochem. Cytochem

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Fatty liver is common in men and post-menopausal women, suggesting that estrogen may be involved in liver lipid metabolism. The aim of this study is to be clear the role of estrogen and estrogen receptor alpha (ERα) in fat accumulation during liver regeneration using the 70% partial hepatectomy (PHX) model in male, female, ovariectomized (OVX) and E 2-treated OVX (OVX-E 2) rats. Liver tissues were sampled at 0-48 hr after PHX and fat accumulation, fatty acid translocase (FAT/CD36), sterol regulatory element-binding protein (SREBP1c), peroxisome proliferator-activated receptor α (PPARα), proliferative cell nuclear antigen (PCNA) and ERα were examined by Oil Red O, qRT-PCR and immunohistochemistry, respectively. Hepatic fat accumulation was abundant in female and OVX-E 2 compared to male and OVX rats. FAT/CD36 expression was observed in female, OVX and OVX-E 2 at 0-12 hr after PHX, but not in male rats. At 0 hr, SREBP1c and PPARα were elevated in female and male rats, respectively, but were decreased after PHX in all rats. The PCNA labeling index reached a maximum at 36 hr and 48 hr in OVX-E 2 and OVX rats, respectively. ERα expression in OVX-E 2 was higher than OVX at 0-36 hr after PHX. In conclusion, these results indicated that estrogen and ERα might play an important role in fat accumulation related to FAT/CD36 during early phase of rat liver regeneration.

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Section: Discussionsupporting

confidence: 49%

The Effect of Estrogen on Hepatic Fat Accumulation during Early Phase of Liver Regeneration after Partial Hepatectomy in Rats

Srisowanna

Choijookhuu

Yano

et al. 2019

Acta Histochem. Cytochem

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“…Thus, the gender differences in ApoE distribution reported here may account in part for gender differences in fatty acid metabolism described previously. [63][64][65][66] We speculate that the gender-specific differences in liver lobular gradients for ApoE mRNA reported here may help explain some of the well-known sex differences observed in susceptibility to atherosclerosis, cholesterol gallstone disease, autoimmune disorders, and Alzheimer' s disease, all of which are known to be influenced by ApoE. HEPATOLOGY Vol.…”

Section: Discussionmentioning

confidence: 99%

Differential expression of apolipoprotein E messenger RNA within the rat liver lobule determined byin situ hybridization

Massimi¹,

Lear²,

Williams³

et al. 1999

Hepatology

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Apolipoprotein (Apo) E plays a key role in the metabolism of lipoproteins. It also modulates immunoregulation, cell growth and differentiation and the response to nerve injury. The liver is a major site of ApoE synthesis. Most of the circulating ApoE is thought to be of hepatic origin with most synthesized in hepatocytes. We showed that total liver ApoE messenger RNA (mRNA) levels were greater in normal adult female rats than in male and that genderspecific patterns of liver ApoE mRNA expression were present by in situ hybridization. In the male liver, the signal was strongest in the portal area, decreasing toward the central vein with the weakest signal in pericentral hepatocytes, resulting in a hepatic lobular gradient of expression. In female liver, a strong periportal signal also was observed that decreased in Zone 2, similar to that in males, but which then increased in pericentral hepatocytes resulting in a bowl-like distribution in marked contrast with that of the male. The results suggest that ApoE mRNA level is regulated differentially in hepatocytes within the liver plate and that the regulation is gender-dependent. Further, the results suggest that in males, hepatocytes in the portal area are the major contributors of ApoE to the plasma and/or sinusoidal pool, whereas in females, both portal and central area hepatocytes play an equal role. (HEPATOLOGY 1999;29:1549-1555.)

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Heterogeneity of kinetic parameters of enzymes in situ in rat liver lobules

Noorden

Jonges

1995

Histochem Cell Biol

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In the present review, metabolic compartmentation in liver lobules is discussed as being dynamic and more complex than thus far assumed on the basis of numbers of mRNA or protein molecules or the capacity (zero-order activity) of enzymes. Isoenzyme distribution patterns and local kinetic parameters of enzymes may vary over the different zones of liver lobules. As a consequence, metabolic fluxes in vivo at physiological substrate concentrations may be completely different from those that are assumed on the basis of the number of molecules or the capacity of enzymes present in zones of liver lobules. For a more correct estimation of the levels of metabolic processes in the different compartments of liver tissue, local kinetic parameters and substrate concentrations have to be determined to calculate local metabolic fluxes. Direct measurements of metabolic fluxes in vivo with the use of noninvasive techniques is a promising alternative and the techniques will become increasingly important in future metabolic research.

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Adaptive sex-dependent changes in the zonation of carbohydrate and lipid metabolism in rat liver lobules after partial hepatectomy

Cited by 4 publications

References 66 publications

The Effect of Estrogen on Hepatic Fat Accumulation during Early Phase of Liver Regeneration after Partial Hepatectomy in Rats

The Effect of Estrogen on Hepatic Fat Accumulation during Early Phase of Liver Regeneration after Partial Hepatectomy in Rats

Differential expression of apolipoprotein E messenger RNA within the rat liver lobule determined byin situ hybridization

Heterogeneity of kinetic parameters of enzymes in situ in rat liver lobules

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