Adaptive reversions of a frameshift mutation in arrested Saccharomyces cerevisiae cells by simple deletions in mononucleotide repeats

Heidenreich, Erich; Wintersberger, Ulrike

doi:10.1016/s0027-5107(00)00141-x

Cited by 24 publications

(28 citation statements)

References 19 publications

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“…Especially starvation for lysine is an important condition of stationary-phase mutagenesis studies in yeast during selection for spontaneous revertants of the lys2DBglII allele [24,25,[30][31][32][33]. It is known from the progressive decrease in viability and the progressive accumulation of respiration-deficient cells [34] that such starvation conditions are stressful to the cells.…”

Section: Resultsmentioning

confidence: 99%

Starvation for an essential amino acid induces apoptosis and oxidative stress in yeast

Eisler

Fröhlich

Heidenreich

2004

Experimental Cell Research

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Section: Resultsmentioning

confidence: 99%

Starvation for an essential amino acid induces apoptosis and oxidative stress in yeast

Eisler

Fröhlich

Heidenreich

2004

Experimental Cell Research

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“…In one strain background housing this allele, lack of functional mitochondria does not affect starvation-stress-induced mutations, strongly suggesting that oxidative DNA lesions are not precursors in the mutagenic process (Heidenreich and Wintersberger, 1998). In a remarkable similarity with stress-induced mutations in the lac system, adaptive reversion of the +4 lys2 allele shows a strong bias for −1 deletions in mononucleotide repeats, in contrast to a variety of different mutations occurring in the same locus in growing cells (Heidenreich and Wintersberger, 2001), although, when assayed in the other strain, both the stress-induced and generation-dependent mutations were mostly deletions in simple repeats (Greene and Jinks-Robertson, 1999). Yeast strains can be quite different genetically, and we will confine the rest of this discussion to the strain used by Heidenreich andcolleagues (2003, 2004), only because more information exists about the mechanism of mutagenesis in it.…”

Section: Stress-induced Mutations In Yeastmentioning

confidence: 92%

Mutation as a Stress Response and the Regulation of Evolvability

Galhardo

Hastings

Rosenberg

2007

Critical Reviews in Biochemistry and Molecular Biology

561

450

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Our concept of a stable genome is evolving to one in which genomes are plastic and responsive to environmental changes. Growing evidence shows that a variety of environmental stresses induce genomic instability in bacteria, yeast, and human cancer cells, generating occasional fitter mutants and potentially accelerating adaptive evolution. The emerging molecular mechanisms of stressinduced mutagenesis vary but share telling common components that underscore two common themes. The first is the regulation of mutagenesis in time by cellular stress responses, which promote random mutations specifically when cells are poorly adapted to their environments, i.e., when they are stressed. A second theme is the possible restriction of random mutagenesis in genomic space, achieved via coupling of mutation-generating machinery to local events such as DNA-break repair or transcription. Such localization may minimize accumulation of deleterious mutations in the genomes of rare fitter mutants, and promote local concerted evolution. Although mutagenesis induced by stresses other than direct damage to DNA was previously controversial, evidence for the existence of various stress-induced mutagenesis programs is now overwhelming and widespread. Such mechanisms probably fuel evolution of microbial pathogenesis and antibiotic-resistance, and tumor progression and chemotherapy resistance, all of which occur under stress, driven by mutations. The emerging commonalities in stress-induced-mutation mechanisms provide hope for new therapeutic interventions for all of these processes.

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“…To the best of our knowledge, this finding has not been described in any study of stationary-phase yeast. This cross-genome DNA synthesis can be the result of several processes, or their (Heidenreich & Wintersberger, 2001;Nouspikel & Hanawalt, 2002;Heidenreich et al, 2003;Heidenreich & Eisler, 2004;Ebina & Levin, 2007). Indeed, we observed Ty1 labelling, indicative of possible retrotransposition during stationary-phase, which was probably the result of the stationary-phase-exerted stress (Ebina & Levin, 2007).…”

Section: Discussionmentioning

confidence: 69%

Genome-wide analysis of DNA turnover and gene expression in stationary-phase Saccharomyces cerevisiae

et al. 2010

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Exponential-phase yeast cells readily enter stationary phase when transferred to fresh, carbondeficient medium, and can remain fully viable for up to several months. It is known that stationaryphase prokaryotic cells may still synthesize substantial amounts of DNA. Although the basis of this phenomenon remains unclear, this DNA synthesis may be the result of DNA maintenance and repair, recombination, and stress-induced transposition of mobile elements, which may occur in the absence of DNA replication. To the best of our knowledge, the existence of DNA turnover in stationary-phase unicellular eukaryotes remains largely unstudied. By performing cDNA-spotted (i.e. ORF) microarray analysis of stationary cultures of a haploid Saccharomyces cerevisiae strain, we demonstrated on a genomic scale the localization of a DNA-turnover marker [5-bromo-29-deoxyuridine (BrdU); an analogue of thymidine], indicative of DNA synthesis in discrete, multiple sites across the genome. Exponential-phase cells on the other hand, exhibited a uniform, total genomic DNA synthesis pattern, possibly the result of DNA replication. Interestingly, BrdUlabelled sites exhibited a significant overlap with highly expressed features. We also found that the distribution among chromosomes of BrdU-labelled and expressed features deviates from random distribution; this was also observed for the overlapping set. Ty1 retrotransposon genes were also found to be labelled with BrdU, evidence for transposition during stationary phase; however, they were not significantly expressed. We discuss the relevance and possible connection of these results to DNA repair, mutation and related phenomena in higher eukaryotes.

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Adaptive reversions of a frameshift mutation in arrested Saccharomyces cerevisiae cells by simple deletions in mononucleotide repeats

Cited by 24 publications

References 19 publications

Starvation for an essential amino acid induces apoptosis and oxidative stress in yeast

Starvation for an essential amino acid induces apoptosis and oxidative stress in yeast

Mutation as a Stress Response and the Regulation of Evolvability

Genome-wide analysis of DNA turnover and gene expression in stationary-phase Saccharomyces cerevisiae

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