Adaptive response of cultured fibroblasts to hyperosmolarity

Petronini, Pier Giorgio; Tramacere, Mariarosaria; Kay, John E.; Borghetti, Angelo F.

doi:10.1016/0014-4827(86)90542-2

Cited by 36 publications

(21 citation statements)

References 17 publications

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“…Osmotic swelling or shrinkage is rapid (minutes) and alters the water content of the cell, leading to corresponding dilution or concentration of intracellular ions. Second, if the ionic composition of the external solution is altered, changes in the ion gra-dients across the plasma membrane may alter intracellular concentrations directly or indirectly (Lew and Bookchin, 1986;Petronini et al, 1986;.…”

Section: Discussionmentioning

confidence: 99%

Regulation of matrix synthesis rates by the ionic and osmotic environment of articular chondrocytes

Urban

Hall

Gehl

1993

Journal Cellular Physiology

269

292

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Chondrocytes in cartilage are embedded in a matrix containing a high concentration of proteoglycans and hence of fixed negative charges. Their extracellular ionic environment is thus different from that of most cells, with extracellular Na+ being 250-350 mM and extracellular osmolality 350-450 mOsm. When chondrocytes are isolated from the matrix and incubated in standard culture medium (DMEM; osmolality 250-280 mOsm), their extracellular environment changes sharply. We incubated isolated bovine articular chondrocytes and cartilage slices in DMEM whose osmolality was altered over the range 250-450 mOsm by Na+ or sucrose addition. 35S-sulphate and 3H-proline incorporation rates were at a maximum when the extracellular osmolality was 350-400 mOsm for both freshly isolated chondrocytes and for chondrocytes in cartilage. The incorporation rate per cell of isolated chondrocytes was only 10% that of chondrocytes in situ both 4 and 24 hours after isolation. For freshly isolated chondrocytes, the rate increased 30-50% in DMEM to which NaCl or sucrose had been added to increase osmolality. In chondrocytes incubated overnight in DMEM, the rate was greatest in DMEM of normal osmolality and fell from the maximum in proportion to the change in osmolality. The effects of sucrose addition on incorporation rates were similar but not identical to those of Na+ addition. Changes in cell volume might be linked to changes in synthesis rates since the cell volume of chondrocytes (measured by Coulter-counter) increased 30-40% when the cells were removed from their in situ environment into DMEM. Synthesis rates can thus be partly regulated by changes in extracellular osmolality, which in cartilage is controlled by proteoglycan concentration. This provides a mechanism by which the chondrocytes can rapidly respond to changes in extracellular matrix composition.

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Section: Discussionmentioning

confidence: 99%

Regulation of matrix synthesis rates by the ionic and osmotic environment of articular chondrocytes

Urban

Hall

Gehl

1993

Journal Cellular Physiology

269

292

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“…Intracellular Na + and K + concentrations ([Na + ] i and [K + ] i respectively) were estimated using the procedure described by Owen and Villereal [14], with slight modifications [15].…”

Section: Determination Of Cell Volume and Intracellular Univalent Catmentioning

confidence: 99%

Activation of heat-shock transcription factor 1 by hypertonic shock in 3T3 cells

Alfieri

Petronini

Urbani

et al. 1996

Biochemical Journal

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The exposure of 3T3 cells to a medium made hypertonic by the addition of NaCl induced activation of a heat-shock transcription factor (HSF). This activation, as monitored by gel-mobility-shift assays, occurred within 10 min of hypertonic shock and was dose-dependent in relation to the osmotic strength of the medium up to 0.7 osM. Competition analysis indicated that the effect of hypertonic shock on HSF binding activity was specific. The magnitude of the heat-shock element (HSE)-HSF binding induced by incubating the cells in a 0.7 osM medium was comparable in intensity and time course with that induced by a 44 mC heat shock. Following removal of the stressors, the decrease in HSF-HSE binding was more rapid in hypertonicity-shocked than in heat-shocked cells. Treatment of the cells with cycloheximide did not inhibit HSF-HSE binding, indicating that the activation was independent of new protein synthesis. By using a

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“…(a) General protein synthesis decreases (Petronini et al, 1986). (b) Cell proliferation decreases (Petronini et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

Effect of betaine on HSP70 expression and cell survival during adaptation to osmotic stress

Petronini

Angelis

Borghetti

et al. 1993

Biochemical Journal

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Induced expression of the HSP70 gene in 3T3 and SV-3T3 cells was monitored by measurements of the synthesis of HSP70 and of the cellular contents of both HSP70 and its mRNA. The presence of betaine (N-trimethylglycine) at concentrations of 2.5-25 mM decreased the induction of HSP70 gene expression caused by incubation of 3T3 and SV-3T3 cells in hypertonic (0.5 osM) medium. This effect was accompanied by an enhancement of SV-3T3 cell adaptation, assayed by colony formation, to the hyperosmotic conditions. In contrast, the presence of betaine did not affect HSP70 gene expression induced in these

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Adaptive response of cultured fibroblasts to hyperosmolarity

Cited by 36 publications

References 17 publications

Regulation of matrix synthesis rates by the ionic and osmotic environment of articular chondrocytes

Regulation of matrix synthesis rates by the ionic and osmotic environment of articular chondrocytes

Activation of heat-shock transcription factor 1 by hypertonic shock in 3T3 cells

Effect of betaine on HSP70 expression and cell survival during adaptation to osmotic stress

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