Adaptive protection against the induction of oxidative DNA damage after hyperbaric oxygen treatment

Rothfuß, Andreas; Dennog, Claudia; Speit, Günter

doi:10.1093/carcin/19.11.1913

Cited by 67 publications

(38 citation statements)

References 35 publications

(34 reference statements)

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“…These findings are in agreement with the results by other authors who concluded that smoking of less than 20 cigarettes per day had no effect on the change of MN frequency, and that only a few cigarettes per day stimulated protective cell response (Gourabi and Mozdarani 1998;Rothfuss et al 1998). In our study, mothers smoked 5-10 cigarettes per day.…”

Section: Discussionsupporting

confidence: 93%

Treatment of Pregnant Women with a Betamimetic and Verapamil Increases the Micronuclei Frequency in Umbilical Cord Blood Lymphocytes

Grujičić

Milošević-Djordjević

Arsenijevic

et al. 2008

Tohoku J. Exp. Med.

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In prevention of preterm labor, betamimetics are used in gynecological practice mostly combined with antiarrhythmic verapamil because of their therapeutic cardiovascular side effects. The aim of this study was to investigate the influence of a betamimetic (ritodrine hydrochloride, fenoterol or hexoprenaline) and verapamil (administered to mothers) on the frequency of micronuclei (MN) in umbilical cord blood lymphocytes of neonates, using cytokinesis-block micronucleus test. The analyzed sample included 23 babies whose mothers received the therapy and 30 control babies whose mothers received no therapy. The average MN frequency was significantly higher in the neonates whose mothers received the therapy (8.13 ± 2.69 MN/1000 BN cells), in comparison with the baseline frequency in untreated controls (3.30 ± 2.63 MN/1000 BN cells), with probability p < 0.05. The highest MN frequency was found in neonates whose mothers received fenoterol and verapamil (2.8-fold i.e. 9.10 ± 3.00 MN/1000 BN cells), while ritodrine hydrochloride and hexoprenaline combined with verapamil induced 2.3-fold and 2.2-fold higher MN values than in controls (7.50 ± 3.33 MN/1000 BN cells and 7.29 ± 0.95 MN/1000 BN cells). Multiple linear regression analysis showed that MN frequency was affected only by the maternal therapeutic treatment, while the neonates' sex, maternal age, cigarette smoking, and therapeutic doses did not affect the MN frequency in umbilical lymphocytes of neonates. We conclude that the treatment of pregnant women with a betamimetic and verapamil significantly increases the MN frequency in umbilical cord blood lymphocytes of neonates, regardless to therapeutic doses.umbilical cord blood lymphocytes; micronuclei; betamimetic; verapamil.Tohoku

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Section: Discussionsupporting

confidence: 93%

Treatment of Pregnant Women with a Betamimetic and Verapamil Increases the Micronuclei Frequency in Umbilical Cord Blood Lymphocytes

Grujičić

Milošević-Djordjević

Arsenijevic

et al. 2008

Tohoku J. Exp. Med.

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“…We found no difference in DNA repair capacity using any of these tests. These assays were chosen because they are rapid and simple, and they have successfully been employed to screen for radiation-sensitive individuals and individuals with cancer-prone syndromes (Rothfuss et al, 1998;Djuzenova et al, 1999;Kuhne et al, 2004). As a panel, the three tests measure both the immediate effect of radiation on DNA breakage, and DNA repair capacity up to 24 h post-irradiation.…”

Section: Discussionmentioning

confidence: 99%

DNA repair capacity as a possible biomarker of breast cancer risk in female BRCA1 mutation carriers

et al. 2007

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The BRCA1 gene product helps to maintain genomic integrity through its participation in the cellular response to DNA damage: specifically, the repair of double-stranded DNA breaks. An impaired cellular response to DNA damage is a plausible mechanism whereby BRCA1 mutation carriers are at increased risk of breast cancer. Hence, an individual's capacity to repair DNA may serve as a useful biomarker of breast cancer risk. The overall aim of the current study was to identify a biomarker of DNA repair capacity that could distinguish between BRCA1 mutation carriers and non-carriers. DNA repair capacity was assessed using three validated assays: the single-cell alkaline gel electrophoresis (comet) assay, the micronucleus test, and the enumeration of g-H2AX nuclear foci. DNA repair capacity of peripheral blood lymphocytes from 25 cancer-free female heterozygous BRCA1 mutation carriers and 25 noncarrier controls was assessed at baseline and following cell exposure to g -irradiation (2 Gy). We found no significant differences in the mean tail moment, in the number of micronuclei or in the number of g-H2AX nuclear foci between the carriers and non-carriers at baseline, and following g-irradiation. These data suggest that these assays are not likely to be useful in the identification of women at a high risk for breast cancer. British Journal of Cancer (2007) The inheritance of a deleterious mutation in the breast cancer susceptibility gene, BRCA1, has been associated with a lifetime risk of breast cancer of between 45 and 87% (Ford et al, 1998;Antoniou et al, 2003). Genetic, reproductive, and environmental factors have all been suggested to influence breast cancer risk in BRCA1 mutation carriers (reviewed in Narod and Offit (2005)). The use of breast cancer as an endpoint to evaluate the protective role of potential modifying factors is not always feasible. Thus, there is a need to ascertain biomarkers of cancer susceptibility in these women. In turn, the identification of a valid biomarker of breast cancer risk would allow us to identify mutation carriers and help improve our ability to target, and to evaluate the effect of both dietary and lifestyle alterations, as well as, medical diagnostic and therapeutic interventions on breast cancer risk.The BRCA1 protein plays a vital role in maintaining genomic stability because of its key role in the repair of double-stranded DNA breaks by homologous recombination (reviewed in Welcsh et al (2000)). If double-stranded DNA breaks are left unrepaired, or are repaired inaccurately, aberrant chromosome breaks, deletions, and translocations may accumulate. Thus, an impaired cellular response to DNA damage appears to be a plausible mechanism whereby BRCA1 mutation carriers are at an increased risk of breast cancer (Scott, 2004). Hence, the evaluation of an individual's capacity to repair DNA may serve as a biomarker of breast cancer risk in carriers of these mutations.Two previous studies have shown higher levels of chromosomal aberrations and chromosomal breaks among women with a BRCA...

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“…The authors assumed that such result is due to a primary DNA repair mechanism 12 . In a later study, the same group showed that a single HBO session induced adaptive response that protected human blood cells from the genotoxic effects caused by hydrogen peroxide 13 . The great variety of HBO protocols described may confirm differences in presence of MN founded in many studies.…”

Section: Discussionmentioning

confidence: 96%

“…Some studies analyzed HBO mutagenic potential and showed no significant MN increase both in humans 12,13 and animals 14 . However, divergent results were shown in cell cultures 14 and human samples 15 .…”

Section: Discussionmentioning

confidence: 99%

“…Some studies analyzed HBO mutagenic potential and divergent results were shown [12][13][14][15] . Due to the important role in micronucleated cells splenic capture and since little is known about the impact of HBO on MN frequency on partially splenectomized animals 16 , we evaluate the mutagenic potential of a HBO protocol by means of a micronucleus test performed in the peripheral blood of Rattus norvegicus Wistar rats submitted to ESTPI, according to techniques already described [17][18][19][20][21] .…”

Section: Introductionmentioning

confidence: 99%

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Micronucleus test in peripheral blood of rats treated with hyperbaric oxygen after subtotal splenectomy preserving the lower pole

et al. 2015

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PURPOSE:To assess the mutagenic potential of the oxygen inhalation therapy (HBO), by means of the micronucleus test, performed in peripheral blood of rats that underwent subtotal splenectomy with lower pole preservation (ESTPI), after HBO sessions or simulations. METHODS:Eighteen male Wistar rats, were distributed into three groups of six animals: group 1 -submitted to ESTPI and HBO sessions; group 2 -submitted to ESTPI and HBO simulations; group 3 -underwent cyclophosphamide administration. In groups 1 and 2, blood samples from the animals' tails were collected before surgery (T0) and immediately after the 13th HBO session or simulation (T1). In group 3, tail blood samples were collected from animals before (T0) and 24 hours after (T1) cyclophosphamide (CP) delivery.The number of micronucleated normochromatic erythrocytes (MNNCE) was determined by blind counting 2000 normochromatic erythrocytes (NCE) per animal. RESULTS:Micronuclei average after CP delivery in group 3 was higher than before its use, thus confirming the mutagenic activity of this drug (p=0.01). In groups 1 and 2, no significant difference in the average of Micronuclei was observed when comparing it to blood samples before and after the 13th HBO session or simulation. CONCLUSION:The treatment protocol used in this study did not induce Micronucleus formation in animals submitted to ESTPI and HBO treatment or simulation.Key words: Splenectomy. Mutagenicity Tests. Oxygen Inhalation Therapy. Rats. Micronucleus test in peripheral blood of rats treated with hyperbaric oxygen after subtotal splenectomy preserving the lower pole

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Adaptive protection against the induction of oxidative DNA damage after hyperbaric oxygen treatment

Cited by 67 publications

References 35 publications

Treatment of Pregnant Women with a Betamimetic and Verapamil Increases the Micronuclei Frequency in Umbilical Cord Blood Lymphocytes

Treatment of Pregnant Women with a Betamimetic and Verapamil Increases the Micronuclei Frequency in Umbilical Cord Blood Lymphocytes

DNA repair capacity as a possible biomarker of breast cancer risk in female BRCA1 mutation carriers

Micronucleus test in peripheral blood of rats treated with hyperbaric oxygen after subtotal splenectomy preserving the lower pole

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