Adaptive optics line-scan OCT for high-speed imaging of retinal structure and function (Conference Presentation)

Pandiyan, Vimal Prabhu; Bertelli, Aiden Maloney; Kuchenbecker, James A.; Boyle, Kevin C.; Ling, Taotao; Park, B. Hyle; Palanker, Daniel; Roorda, Austin; Sabesan, Ramkumar

doi:10.1117/12.2545204

Cited by 3 publications

(3 citation statements)

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“…In point-scanning swept-source systems with sweep rates ≥100 kHz and point-or line-illumination scanning [28] spectrometer-based systems, the high-frequency regime is typically neglected, but the OPL fluctiations between the scan rate and volume rate are encoded as spatially varying phase noise. Parallelization in our system guarantees, in principle, that OPL fluctuations are spatially constant.…”

Section: Spectral Analysis Of System Vibrationsmentioning

confidence: 99%

Kilohertz retinal FF-SS-OCT and flood imaging with hardware-based adaptive optics

Valente

Vienola

Zawadzki

et al. 2020

Preprint

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A retinal imaging system was designed for full-field (FF) swept-source (SS) optical coherence tomography (OCT) with cellular resolution. The system incorporates a real-time adaptive optics (AO) subsystem and a very high speed CMOS sensor, and is capable of acquiring volumetric images of the retina at rates up to 1 kHz. While digital aberration correction (DAC) is an attractive potential alternative to AO, it has not yet been shown to provide resolution of cones in the fovea, where early detection of functional deficits is most critical. Here we demonstrate that FF-SS-OCT with hardware AO permits resolution of foveal cones, with volume rates adequate to measure light-evoked changes in photoreceptors. With the reference arm blocked, the system can operate as kilohertz AO flood illumination fundus camera with adjustable temporal coherence and is expected to allow measurement of light-evoked changes caused by common path interference in photoreceptor outer segments (OS). In this work, we describe the system’s optical design, characterize its performance, and demonstrate its ability to produce images of the human photoreceptor mosaic.

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Section: Spectral Analysis Of System Vibrationsmentioning

confidence: 99%

Kilohertz retinal FF-SS-OCT and flood imaging with hardware-based adaptive optics

Valente

Vienola

Zawadzki

et al. 2020

Preprint

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“…Although other reported systems demonstrate the use of Powell-lens to generate the line-field illumination, the reliability of the beam mixing of the sample and reference beam can become more challenging [2], and conventional cylinder lenses are used. The line-field sample illumination is important to obtain sufficient photon flux for structural detection in the sample; the careful design of the detector arm and anamorphic beam shaping can help to improve further the signal properties [12]. The spectroscopic detection in the described system is achieved using an optical grating from Wasatch Photonics with 1800 lines per millimeter.…”

Section: Introductionmentioning

confidence: 99%

In-vivo real-time imaging of the human cornea and retina with high-speed line-field optical coherence tomography

Neuhaus,

Ni,

Khan

et al. 2024

Ophthalmic Technologies XXXIV

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A line-field optical coherence tomography (LFOCT) application is implemented for real-time in vivo corneal and retinal imaging. In contrast to other described systems of LFOCT that use single-shot high-speed cameras, we describe the first results utilizing a camera with continuous high-speed data transfer and display. The system is based on a previously published design using a center wavelength of 840 nm and a bandwidth of 50 nm. The system's B-frame and en-face display speed reaches up to 5000 frames per second corresponding to 2,500,000 A-lines. A visible light camera is used to detect the interferometric signal to reduce costs and improve optomechanical integration. Balancing the sensitivity vs. acquisition speed allows continuous high data transfer and processing rates and simplifies the implementation as a bedside system. Higher frame rates are important for scan positioning on non-compliant subjects such as infants and children.

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“…Using a swept-source for LFOCT achieve a speed of 10 volumes per seconds 6,7 . High-speed spectral domain LFOCT systems with using a Photron, FASTCAM Mini Ax200 have been demonstrated, however high acquisition speeds may compromise sensitivity and intermittent data storage does not allow real-time display of images [8][9][10] . We identified the need for an LFOCT configuration that is capable of real-time imaging and is scalable for ophthalmic applications yet can implement as many optical enhancements as possible at reasonable costs and effort.…”

Section: Introductionmentioning

confidence: 99%

Real-time line-field OCT using low-cost high-speed camera

Neuhaus

Ni²,

Khan³

et al. 2023

Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVII

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A real-time high-speed line-field optical coherence tomography (LFOCT) configuration at 5,000 B-frames or 2,500,000 A-lines per second for clinical applications is described. The development of the LFOCT technology has experienced increasing interest despite the challenge of achieving sufficient sensitivity and resolution due to crosstalk. Publications of research systems usually apply expensive infrared cameras to improve image quality. The costs, complexity, and lack of real-time data transfer of dedicated highspeed IR cameras make it challenging to employ LFOCT for a more comprehensive application range. We demonstrate using a comparatively low-cost, high-speed camera for real-time data transfer and image display in conjunction with a light source at a center wavelength of 840 nm and a bandwidth of 50 nm. Although the camera and available sample power limits the sensitivity (75 dB), we can demonstrate imaging on a human fingertip (in vivo) and the cornea of a rabbit eye. Further development of the system will focus on compensating for the camera's limited sensitivity and improving resolution to target clinical in vivo imaging such as ophthalmic applications.

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Adaptive optics line-scan OCT for high-speed imaging of retinal structure and function (Conference Presentation)

Cited by 3 publications

References 0 publications

Kilohertz retinal FF-SS-OCT and flood imaging with hardware-based adaptive optics

Kilohertz retinal FF-SS-OCT and flood imaging with hardware-based adaptive optics

In-vivo real-time imaging of the human cornea and retina with high-speed line-field optical coherence tomography

Real-time line-field OCT using low-cost high-speed camera

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