Adaptive optics in single objective inclined light sheet microscopy enables three-dimensional localization microscopy in adult Drosophila brains

Hung, Shih-Te; Rosell, Arnau Llobet; Jurriens, Daphne; Siemons, Marijn; Soloviev, Oleg; Kapitein, Lukas C.; Grußmayer, Kristin S.; Neukomm, Lukas J.; Verhaegen, Michel; Smith, Carlas

doi:10.3389/fnins.2022.954949

Cited by 5 publications

(2 citation statements)

References 54 publications

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“…Hung et al combined sensorless adaptive optics (AO), in-situ 3D-PSF calibration, and a single-objective lens inclined light sheet microscope (SOLEIL), termed (AO-SOLEIL), to mitigate deep tissue-induced deteriorations [84]. They applied AO-SOLEIL on several dSTORM samples including brains of adult Drosophila.…”

Section: Illumination Approachesmentioning

confidence: 99%

Light-sheet fluorescent microscopy: fundamentals, developments and applications

Kafian,

Mozaffari-Jovin,

Bagheri

et al. 2023

Phys. Scr.

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Deep volumetric microscopy of live objects plays a critical role in biology and medicine. To this end, development of rapid and non-invasive optical methods for 3-dimensional (3D) imaging is still demanding. In this way, light-sheet fluorescence microscopy (LSFM) has emerged as a volumetric microscopy method having high spatial-temporal resolution for imaging of samples within dimensions from submicron to few centimeters with minimum induced photo damaging. Unique features of LSFM allow for its modification and deployments in various fields including developmental biology, pathology, and microfluidics. Considering the wide spectrum of LSFM users, this tutorial review article explains basic concepts and design considerations of LSFM and provides a detailed analysis of various optical configurations of LSFM. Major developments of LSFM for adoption in both research and clinical applications including tissue imaging, diagnostics, and cytometry are also explained. In addition, various designs of light-sheet fluorescent microscopes for use as a stand-alone microscopy unit and an add-on device are discussed.

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Section: Illumination Approachesmentioning

confidence: 99%

Light-sheet fluorescent microscopy: fundamentals, developments and applications

Kafian,

Mozaffari-Jovin,

Bagheri

et al. 2023

Phys. Scr.

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“…AO has been successfully applied to raster scanning microscopes [5][6][7], and has yielded up to an order of magnitude improvement in fluorescence signal in multiphoton systems [8,9]. It has also found applications in all major superresolution technologies, namely structured illumination microscopy (SIM) [10][11][12], stimulated emission depletion (STED) [13,14] and single molecule localization microscopy [15][16][17][18][19][20]. However, its adoption in the field of LSFM has been slow.…”

Section: Introductionmentioning

confidence: 99%

Adaptive Optics in an Oblique Plane Microscope

McFadden,

Marin,

Chen

et al. 2024

Preprint

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Adaptive optics (AO) can restore diffraction limited performance when imaging beyond superficial cell layers in vivo and in vitro, and as such is of interest for advanced 3D microscopy methods such as light-sheet fluorescence microscopy (LSFM). In a typical LSFM system, the illumination and detection paths are separate and subject to different optical aberrations. To achieve optimal microscope performance, it is necessary to sense and correct these aberrations in both light paths, resulting in a complex microscope system. Here, we show that in an oblique plane microscope (OPM), a type of LSFM with a single primary objective lens, the same deformable mirror can correct both the illumination and fluorescence detection. Besides reducing the complexity, we show that AO in OPM also restores the relative alignment of the light-sheet and focal plane, and that a projection imaging mode can stabilize and improve the wavefront correction in a sensorless AO format. We demonstrate OPM with AO on fluorescent nanospheres and by imaging the vasculature and cancer cells in zebrafish embryos embedded in a glass capillary, restoring diffraction limited resolution and improving the signal strength twofold.

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Applications of Lightsheet Fluorescence Microscopy by High Numerical Aperture Detection Lens

Shih,

Tang,

Chen

et al. 2024

J. Phys. Chem. B

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This Review explores the evolution, improvements, and recent applications of Light Sheet Fluorescence Microscopy (LSFM) in biological research using a high numerical aperture detection objective (lens) for imaging subcellular structures. The Review begins with an overview of the development of LSFM, tracing its evolution from its inception to its current state and emphasizing key milestones and technological advancements over the years. Subsequently, we will discuss various improvements of LSFM techniques, covering advancements in hardware such as illumination strategies, optical designs, and sample preparation methods that have enhanced imaging capabilities and resolution. The advancements in data acquisition and processing are also included, which provides a brief overview of the recent development of artificial intelligence. Fluorescence probes that were commonly used in LSFM will be highlighted, together with some insights regarding the selection of potential probe candidates for future LSFM development. Furthermore, we also discuss recent advances in the application of LSFM with a focus on high numerical aperture detection objectives for various biological studies. For sample preparation techniques, there are discussions regarding fluorescence probe selection, tissue clearing protocols, and some insights into expansion microscopy. Integrated setups such as adaptive optics, single objective modification, and microfluidics will also be some of the key discussion points in this Review. We hope that this comprehensive Review will provide a holistic perspective on the historical development, technical enhancements, and cutting-edge applications of LSFM, showcasing its pivotal role and future potential in advancing biological research.

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Adaptive optics in single objective inclined light sheet microscopy enables three-dimensional localization microscopy in adult Drosophila brains

Cited by 5 publications

References 54 publications

Light-sheet fluorescent microscopy: fundamentals, developments and applications

Light-sheet fluorescent microscopy: fundamentals, developments and applications

Adaptive Optics in an Oblique Plane Microscope

Applications of Lightsheet Fluorescence Microscopy by High Numerical Aperture Detection Lens

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