Adaptive Optics Confocal Scanning Laser Ophthalmoscope

Lü, Jing; Li, Hao; Shi, Guohua; Zhang, Yudong

doi:10.5772/31117

Cited by 1 publication

(1 citation statement)

References 19 publications

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“…A technique using 3-zone pupil filters 31 has been explored to compensate for resolution degradation with a large pinhole size. 32 Fan-shaped apertures were proposed theoretically to improve axial resolution in confocal microscopy for a given pinhole size. 33 …”

Section: Available Super-resolution Microscopy Techniquesmentioning

confidence: 99%

Super-Resolution Scanning Laser Microscopy Based on Virtually Structured Detection

Zhi

Wang

Yao

2015

Crit Rev Biomed Eng

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Light microscopy plays a key role in biological studies and medical diagnosis. The spatial resolution of conventional optical microscopes is limited to approximately half the wavelength of the illumination light as a result of the diffraction limit. Several approaches—including confocal microscopy, stimulated emission depletion microscopy, stochastic optical reconstruction microscopy, photoactivated localization microscopy, and structured illumination microscopy—have been established to achieve super-resolution imaging. However, none of these methods is suitable for the super-resolution ophthalmoscopy of retinal structures because of laser safety issues and inevitable eye movements. We recently experimentally validated virtually structured detection (VSD) as an alternative strategy to extend the diffraction limit. Without the complexity of structured illumination, VSD provides an easy, low-cost, and phase artifact–free strategy to achieve super-resolution in scanning laser microscopy. In this article we summarize the basic principles of the VSD method, review our demonstrated single-point and line-scan super-resolution systems, and discuss both technical challenges and the potential of VSD-based instrumentation for super-resolution ophthalmoscopy of the retina.

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Section: Available Super-resolution Microscopy Techniquesmentioning

confidence: 99%