Adaptive metabolic responses in a thermostabilized environment: Transgenerational trade-off implications from tropical tilapia

Wang, Min-Chen; Hsu, Mao-Ting; Lin, Chia Feng; Hsu, Shao-Chun; Chen, Ruo-Dong; Lee, Jay-Ron; Chou, Yi-Lin; Tseng, Hung; Furukawa, Fumiya; Hwang, Sheng-Ping L.; Hwang, Pung‐Pung; Tseng, Yung‐Che

doi:10.1016/j.scitotenv.2021.150672

Cited by 2 publications

(2 citation statements)

References 57 publications

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“…The resting metabolic rates (RMRs) of octopus (Octopodidae) and squid (Loliginidae) cephalopods were determined by observing the oxygen consumption rate when the animals were incubated in a closed swim tunnel (an intermittent-flow respirometry system) with continuous water flow (as shown in Supplementary Figure S1). During the experiment, the oxygen consumption of animals was measured until the air saturation level dropped to 70%, a criteria level applicable to embryonic squid and adult fish (Kuan et al, 2022;Wang et al, 2022). In the beginning, the animals were gently transferred to the swimming tunnel and were temporarily adapted for ten minutes.…”

Section: Oxygen Consumption Estimationsmentioning

confidence: 99%

Metabolic trade-offs associated with homeostatic adjustments in pelagic and benthic cephalopods: Comparative evaluations of NH4+/H+ transport machinery in gills

et al. 2022

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Cephalopods are ancient mollusks that can be found in many different ecological niches in the ocean ranging from the intertidal zone to the deep-sea abyss. In order to adapt to a lifestyle in various habitats, cephalopods have evolved a variety of locomotory modes to accommodate their respective habitats. Most cephalopods have relatively high metabolic rates due to their less efficient swimming mode by jet propulsion. This lifestyle is characterized by a high level of energy expenditure, fueled exclusively by protein diets that are rapidly digested and may produce metabolic nitrogenous waste NH3/NH4 +accumulation and acid-base disturbances. This study observed that the NH4 +transport rate in pelagic bigfin reef squid (Sepioteuthis lessoniana) is two times faster than benthic common octopus (Octopus vulgaris). Inhibition of Na+/H+ exchangers (NHEs) showed significant disruption of NH4 + and H+ excretory processes in gills of octopus but not in squid. However, inhibition of vacuolar-type H+-ATPase (VHA) significantly disrupts NH4 + and H+ transport rates in gills of both animals. Accordingly, for NH4 + and H+ homeostasis, benthic octopus with lower aerobic respiration rates utilize both active and Na+-driven secondary transport machinery. In order to avoid NH4 + accumulated in the blood, pelagic squids with higher aerobic respiration rates prefer active NH4 + and H+ transport mechanisms that consume ATP intensively.

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Section: Oxygen Consumption Estimationsmentioning

confidence: 99%

Metabolic trade-offs associated with homeostatic adjustments in pelagic and benthic cephalopods: Comparative evaluations of NH4+/H+ transport machinery in gills

et al. 2022

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“…Cell metabolism can be divided into pathways of substance metabolism and energy metabolism, which denote the ability of cells to exchange substances and transfer energy, and ultimately gives the organism its different phenotypic traits ( DeBerardinis et al, 2008 ). Most studies have described key metabolic mechanisms that are vital to animal growth, but, owing to the complex metabolic network of an organism, elaboration of the relationship between metabolism and growth has largely focused on descriptions of signaling pathways ( Yang et al, 2008 ; Mihaylova and Shaw, 2011 ; Wang M.-C. et al, 2022 , Wang et al, 2022 M. ; Yin et al, 2022 ). However, with the advancement of genomics and transcriptome analysis now provide feasibility for the exploration of key genes that determine key mechanisms in biological metabolism.…”

Section: Introductionmentioning

confidence: 99%

Comparing Transcriptomes Reveals Key Metabolic Mechanisms in Superior Growth Performance Nile Tilapia (Oreochromis niloticus)

et al. 2022

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Metabolic capacity is intrinsic to growth performance. To investigate superior growth performance in Nile tilapia, three full-sib families were bred and compared at the biochemical and transcriptome levels to determine metabolic mechanisms involved in significant growth differences between individuals under the same culture environment and feeding regime. Biochemical analysis showed that individuals in the higher growth group had significantly higher total protein, total triglyceride, total cholesterol, and high- and low-density lipoproteins, but significantly lower glucose, as compared with individuals in the lower growth group. Comparative transcriptome analysis showed 536 differentially expressed genes (DEGs) were upregulated, and 622 DEGs were downregulated. These genes were significantly enriched in three key pathways: the tricarboxylic acid cycle (TCA cycle), fatty acid biosynthesis and metabolism, and cholesterol biosynthesis and metabolism. Conjoint analysis of these key pathways and the biochemical parameters suggests that Nile tilapia with superior growth performance have higher ability to consume energy substrates (e.g., glucose), as well as higher ability to biosynthesize fatty acids and cholesterol. Additionally, the fatty acids biosynthesized by the superior growth performance individuals were less active in the catabolic pathway overall, but were more active in the anabolic pathway, and might be used for triglyceride biosynthesis to store excess energy in the form of fat. Furthermore, the tilapia with superior growth performance had lower ability to convert cholesterol into bile acids, but higher ability to convert it into sterols. We discuss the molecular mechanisms of the three key metabolic pathways, map the pathways, and note key factors that may impact the growth of Nile tilapia. The results provide an important guide for the artificial selection and quality enhancement of superior growth performance in tilapia.

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Adaptive metabolic responses in a thermostabilized environment: Transgenerational trade-off implications from tropical tilapia

Cited by 2 publications

References 57 publications

Metabolic trade-offs associated with homeostatic adjustments in pelagic and benthic cephalopods: Comparative evaluations of NH4+/H+ transport machinery in gills

Metabolic trade-offs associated with homeostatic adjustments in pelagic and benthic cephalopods: Comparative evaluations of NH4+/H+ transport machinery in gills

Comparing Transcriptomes Reveals Key Metabolic Mechanisms in Superior Growth Performance Nile Tilapia (Oreochromis niloticus)

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