Abstract:SUMMARYAutomatic detection of immunoreactive areas in fluorescence microscopic images is becoming a key technique in the field of biology including neuroscience, although it is still challenging because of several reasons such as low signal-to-noise ratio and contrast variation within an image. In this study, we developed a new algorithm that exhaustively detects co-localized areas in multi-channel fluorescence images, where shapes of target objects may differ among channels. Different adaptive binarization th… Show more
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