Adaptive laboratory evolution of the fast-growing cyanobacterium Synechococcus elongatus PCC 11801 for improved solvent tolerance

Srivastava, Vaibhav; Amanna, Ruth; Rowden, Stephen J. L.; Sengupta, Shinjinee; Madhu, Swati; Howe, Christopher J.; Wangikar, Pramod P.

doi:10.1016/j.jbiosc.2020.11.012

Cited by 17 publications

(8 citation statements)

References 75 publications

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“…The engineered strain proved to be a practical and feasible system for cost-effective isobutanol production [ 139 ]. Another strain of S. elongatus PCC 11801 has recently evolved in laboratory conditions through adaptive tolerance to higher concentrations of butanol [ 140 ]. Notwithstanding the above-mentioned advances, cyanobacterial bioethanol and higher alcohols require further improvement to become economically viable at an industrial scale.…”

Section: Recent Metabolic Advances In Microorganism For Biofuel Produ...mentioning

confidence: 99%

Recent advances in metabolic engineering of microorganisms for advancing lignocellulose-derived biofuels

et al. 2022

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Combating climate change and ensuring energy supply to a rapidly growing global population has highlighted the need to replace petroleum fuels with clean, and sustainable renewable fuels. Biofuels offer a solution to safeguard energy security with reduced ecological footprint and process economics. Over the past years, lignocellulosic biomass has become the most preferred raw material for the production of biofuels, such as fuel, alcohol, biodiesel, and biohydrogen. However, the cost-effective conversion of lignocellulose into biofuels remains an unsolved challenge at the industrial scale. Recently, intensive efforts have been made in lignocellulose feedstock and microbial engineering to address this problem. By improving the biological pathways leading to the polysaccharide, lignin, and lipid biosynthesis, limited success has been achieved, and still needs to improve sustainable biofuel production. Impressive success is being achieved by the retouring metabolic pathways of different microbial hosts. Several robust phenotypes, mostly from bacteria and yeast domains, have been successfully constructed with improved substrate spectrum, product yield and sturdiness against hydrolysate toxins. Cyanobacteria is also being explored for metabolic advancement in recent years, however, it also remained underdeveloped to generate commercialized biofuels. The bacterium Escherichia coli and yeast Saccharomyces cerevisiae strains are also being engineered to have cell surfaces displaying hydrolytic enzymes, which holds much promise for near-term scale-up and biorefinery use. Looking forward, future advances to achieve economically feasible production of lignocellulosic-based biofuels with special focus on designing more efficient metabolic pathways coupled with screening, and engineering of novel enzymes.

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Section: Recent Metabolic Advances In Microorganism For Biofuel Produ...mentioning

confidence: 99%

Recent advances in metabolic engineering of microorganisms for advancing lignocellulose-derived biofuels

et al. 2022

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“…High-light- and high-temperature-tolerances are especially important for outdoor applications. Adaptive laboratory evolution (ALE) in combination with systems biology approaches, were successfully applied for Chlamydomonas, Synechocystis , and Synechococcus ( Zhang et al, 2021b ; Srivastava et al, 2021 ; LaPanse et al, 2021 ; Dann et al, 2021 ; Mukherjee et al, 2020 ). Synechocystis sp.…”

Section: Pathway Engineeringmentioning

confidence: 99%

“…PCC 11901 represent a significant progress ( Włodarczyk et al, 2020 ). For the production of fuels or chemicals with (phototrophic) whole-cell factories, solvent tolerance is another desirable trait, for which considerable ALE-based progress recently has been reported for Synechococcus ( Srivastava et al, 2021 ).…”

Section: Pathway Engineeringmentioning

confidence: 99%

Exploitation of Hetero- and Phototrophic Metabolic Modules for Redox-Intensive Whole-Cell Biocatalysis

Theodosiou

Tüllinghoff

Toepel

et al. 2022

Front. Bioeng. Biotechnol.

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The successful realization of a sustainable manufacturing bioprocess and the maximization of its production potential and capacity are the main concerns of a bioprocess engineer. A main step towards this endeavor is the development of an efficient biocatalyst. Isolated enzyme(s), microbial cells, or (immobilized) formulations thereof can serve as biocatalysts. Living cells feature, beside active enzymes, metabolic modules that can be exploited to support energy-dependent and multi-step enzyme-catalyzed reactions. Metabolism can sustainably supply necessary cofactors or cosubstrates at the expense of readily available and cheap resources, rendering external addition of costly cosubstrates unnecessary. However, for the development of an efficient whole-cell biocatalyst, in depth comprehension of metabolic modules and their interconnection with cell growth, maintenance, and product formation is indispensable. In order to maximize the flux through biosynthetic reactions and pathways to an industrially relevant product and respective key performance indices (i.e., titer, yield, and productivity), existing metabolic modules can be redesigned and/or novel artificial ones established. This review focuses on whole-cell bioconversions that are coupled to heterotrophic or phototrophic metabolism and discusses metabolic engineering efforts aiming at 1) increasing regeneration and supply of redox equivalents, such as NAD(P/H), 2) blocking competing fluxes, and 3) increasing the availability of metabolites serving as (co)substrates of desired biosynthetic routes.

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“…The ethanol tolerances of cyanobacteria recorded to date are ~ 1.5% (w/v) [ 98 ], far below the required titre for effective recovery. Efforts have already been made to improve tolerance by engineering and overexpressing transporters [ 99 ] and performing adaptive evolution [ 98 , 100 ].…”

Section: Modulating Flux Through Pyruvatementioning

confidence: 99%

Combinatorial use of environmental stresses and genetic engineering to increase ethanol titres in cyanobacteria

Andrews

Faulkner

Toogood

et al. 2021

Biotechnol Biofuels

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Current industrial bioethanol production by yeast through fermentation generates carbon dioxide. Carbon neutral bioethanol production by cyanobacteria uses biological fixation (photosynthesis) of carbon dioxide or other waste inorganic carbon sources, whilst being sustainable and renewable. The first ethanologenic cyanobacterial process was developed over two decades ago using Synechococcus elongatus PCC 7942, by incorporating the recombinant pdc and adh genes from Zymomonas mobilis. Further engineering has increased bioethanol titres 24-fold, yet current levels are far below what is required for industrial application. At the heart of the problem is that the rate of carbon fixation cannot be drastically accelerated and carbon partitioning towards bioethanol production impacts on cell fitness. Key progress has been achieved by increasing the precursor pyruvate levels intracellularly, upregulating synthetic genes and knocking out pathways competing for pyruvate. Studies have shown that cyanobacteria accumulate high proportions of carbon reserves that are mobilised under specific environmental stresses or through pathway engineering to increase ethanol production. When used in conjunction with specific genetic knockouts, they supply significantly more carbon for ethanol production. This review will discuss the progress in generating ethanologenic cyanobacteria through chassis engineering, and exploring the impact of environmental stresses on increasing carbon flux towards ethanol production.

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Adaptive laboratory evolution of the fast-growing cyanobacterium Synechococcus elongatus PCC 11801 for improved solvent tolerance

Cited by 17 publications

References 75 publications

Recent advances in metabolic engineering of microorganisms for advancing lignocellulose-derived biofuels

Recent advances in metabolic engineering of microorganisms for advancing lignocellulose-derived biofuels

Exploitation of Hetero- and Phototrophic Metabolic Modules for Redox-Intensive Whole-Cell Biocatalysis

Combinatorial use of environmental stresses and genetic engineering to increase ethanol titres in cyanobacteria

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