Adaptive Laboratory Evolution for Multistress Tolerance, including Fermentability at High Glucose Concentrations in Thermotolerant Candida tropicalis

Phommachan, Koudkeo; Keo-oudone, Chansom; Nurcholis, Mochamad; Vongvilaisak, Nookhao; Chanhming, Mingkhuan; Savanhnaly, Vanhnavong; Bounphanmy, Somchanh; Matsutani, Minenosuke; Kosaka, Tomoyuki; Limtong, Savitree; Yamada, Mamoru

doi:10.3390/en15020561

Cited by 9 publications

(4 citation statements)

References 42 publications

(63 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The mutant somehow kept a lower level of acetate compared to the other adapted mutants as seen in the medium containing 160 g L −1 of glucose at 40 • C to 45 • C. Notably, K. marxianus DMKU 3-1042 as well as adapted strains exhibited a stronger resistance to high temperatures (up to 48 • C) on agar plates [16]. It is assumed that only the surface cells of colonies on agar plates are exposed to oxidative stress, which increases with rising temperature [47], and individual cells in liquid media are directly exposed to the stress. The thermal stability of K. marxianus DMKU 3-1042 is one of the most important application criteria for different applications, and this property is crucial for the preparation strategy of the strain or its stabilization.…”

Section: Discussionmentioning

confidence: 99%

Evolutionary Adaptation by Repetitive Long-Term Cultivation with Gradual Increase in Temperature for Acquiring Multi-Stress Tolerance and High Ethanol Productivity in Kluyveromyces marxianus DMKU 3-1042

et al. 2022

Self Cite

View full text Add to dashboard Cite

During ethanol fermentation, yeast cells are exposed to various stresses that have negative effects on cell growth, cell survival, and fermentation ability. This study, therefore, aims to develop Kluyveromyces marxianus-adapted strains that are multi-stress tolerant and to increase ethanol production at high temperatures through a novel evolutionary adaptation procedure. K. marxianus DMKU 3-1042 was subjected to repetitive long-term cultivation with gradual increases in temperature (RLCGT), which exposed cells to various stresses, including high temperatures. In each cultivation step, 1% of the previous culture was inoculated into a medium containing 1% yeast extract, 2% peptone, and 2% glucose, and cultivation was performed under a shaking condition. Four adapted strains showed increased tolerance to ethanol, furfural, hydroxymethylfurfural, and vanillin, and they also showed higher production of ethanol in a medium containing 16% glucose at high temperatures. One showed stronger ethanol tolerance. Others had similar phenotypes, including acetic acid tolerance, though genome analysis revealed that they had different mutations. Based on genome and transcriptome analyses, we discuss possible mechanisms of stress tolerance in adapted strains. All adapted strains gained a useful capacity for ethanol fermentation at high temperatures and improved tolerance to multi-stress. This suggests that RLCGT is a simple and efficient procedure for the development of robust strains.

show abstract

Section: Discussionmentioning

confidence: 99%

Evolutionary Adaptation by Repetitive Long-Term Cultivation with Gradual Increase in Temperature for Acquiring Multi-Stress Tolerance and High Ethanol Productivity in Kluyveromyces marxianus DMKU 3-1042

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…One interesting aspect of ALE is the occurrence of multiple evolved phenotypes, as an indirect result of a particular directed evolution towards a certain goal. For example, Phommachan et al [ 66 ], aimed to use strains of Candida tropicalis to improve their resistance to high glucose concentrations. After long-term cultivation with increasing temperatures (40 °C to 44.5 °C) and the periodical transfer of evolved cultures to media with high glucose concentrations, authors obtained strains that, in addition to fermenting high glucose concentrations, also showed increased tolerance to ethanol, furfural, and hydroxymethylfurfural at high temperatures, and improved xylose-fermenting ability.…”

Section: Evolution Of Non-conventional Yeast Species Through Alementioning

confidence: 99%

Contributions of Adaptive Laboratory Evolution towards the Enhancement of the Biotechnological Potential of Non-Conventional Yeast Species

Fernandes

Osório

Sousa

et al. 2023

JoF

View full text Add to dashboard Cite

Changes in biological properties over several generations, induced by controlling short-term evolutionary processes in the laboratory through selective pressure, and whole-genome re-sequencing, help determine the genetic basis of microorganism’s adaptive laboratory evolution (ALE). Due to the versatility of this technique and the imminent urgency for alternatives to petroleum-based strategies, ALE has been actively conducted for several yeasts, primarily using the conventional species Saccharomyces cerevisiae, but also non-conventional yeasts. As a hot topic at the moment since genetically modified organisms are a debatable subject and a global consensus on their employment has not yet been attained, a panoply of new studies employing ALE approaches have emerged and many different applications have been exploited in this context. In the present review, we gathered, for the first time, relevant studies showing the ALE of non-conventional yeast species towards their biotechnological improvement, cataloging them according to the aim of the study, and comparing them considering the species used, the outcome of the experiment, and the employed methodology. This review sheds light on the applicability of ALE as a powerful tool to enhance species features and improve their performance in biotechnology, with emphasis on the non-conventional yeast species, as an alternative or in combination with genome editing approaches.

show abstract

“…Phommachan et al reported ALE-evolved strains of Candida tropicalis showing tolerance to multiple stress [ 75 ]. They isolated the C. tropicalis strain in Laos, which is a thermotolerant xylose-fermenting yeast, but this species shows sensitivity to a high concentration of glucose.…”

Section: Acquisition Of Stress Tolerance To Microorganisms With Alementioning

confidence: 99%

Adaptive Laboratory Evolution of Microorganisms: Methodology and Application for Bioproduction

Hirasawa

Maeda

2022

Microorganisms

View full text Add to dashboard Cite

Adaptive laboratory evolution (ALE) is a useful experimental methodology for fundamental scientific research and industrial applications to create microbial cell factories. By using ALE, cells are adapted to the environment that researchers set based on their objectives through the serial transfer of cell populations in batch cultivations or continuous cultures and the fitness of the cells (i.e., cell growth) under such an environment increases. Then, omics analyses of the evolved mutants, including genome sequencing, transcriptome, proteome and metabolome analyses, are performed. It is expected that researchers can understand the evolutionary adaptation processes, and for industrial applications, researchers can create useful microorganisms that exhibit increased carbon source availability, stress tolerance, and production of target compounds based on omics analysis data. In this review article, the methodologies for ALE in microorganisms are introduced. Moreover, the application of ALE for the creation of useful microorganisms as cell factories has also been introduced.

show abstract

Adaptive Laboratory Evolution for Multistress Tolerance, including Fermentability at High Glucose Concentrations in Thermotolerant Candida tropicalis

Cited by 9 publications

References 42 publications

Evolutionary Adaptation by Repetitive Long-Term Cultivation with Gradual Increase in Temperature for Acquiring Multi-Stress Tolerance and High Ethanol Productivity in Kluyveromyces marxianus DMKU 3-1042

Evolutionary Adaptation by Repetitive Long-Term Cultivation with Gradual Increase in Temperature for Acquiring Multi-Stress Tolerance and High Ethanol Productivity in Kluyveromyces marxianus DMKU 3-1042

Contributions of Adaptive Laboratory Evolution towards the Enhancement of the Biotechnological Potential of Non-Conventional Yeast Species

Adaptive Laboratory Evolution of Microorganisms: Methodology and Application for Bioproduction

Contact Info

Product

Resources

About