Adaptive immune stimuli altered the cargo proteins of exosomes released by supraneural myeloid body cells in Lampetra japonica

Wang, Hao; Cheng, Xianda; Li, Mengjiao; Li, Wenna; Zhu, Ting; Li, Qingwei

doi:10.1016/j.molimm.2019.04.004

Cited by 5 publications

(5 citation statements)

References 51 publications

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“…Flotillin-1 has indeed been characterised in lamprey and confirmed as a putative marker for EVs (Xu et al, 2017). In Japanese lampern/Arctic lamprey (Lampetra japonica), EVs have previously been isolated from conditioned growth medium of primary supraneural myeloid body cells (SMB cells) and suggested to play immune modulating roles in response to LPS stimulation (Wang et al, 2019a). The current study is though the first to characterise EVs from lamprey plasma.…”

Section: Discussionmentioning

confidence: 71%

Post-translational protein deimination signatures in sea lamprey (Petromyzon marinus) plasma and plasma-extracellular vesicles

Rast

D’Alessio

Kraev

et al. 2021

Developmental & Comparative Immunology

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Section: Discussionmentioning

confidence: 71%

Post-translational protein deimination signatures in sea lamprey (Petromyzon marinus) plasma and plasma-extracellular vesicles

Rast

D’Alessio

Kraev

et al. 2021

Developmental & Comparative Immunology

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“…The BrdU method has advantages over other colorimetric methods that are strongly influenced by the metabolic state of leukocytes and has been applied to the detection of mitosis of leukocytes in vitro in several species of bony fish (23,66). Significantly, our study revealed for the first time that rL-TGF-b2 can exert the bipolar regulation on the proliferation of quiescent and activated SMB cells, which play an important role in the immune response of lampreys (33,67,68). In comparison to SMB cells, L-TGF-b2 can inhibit the proliferation of leukocytes under both quiescent and activated states.…”

Section: Discussionmentioning

confidence: 73%

“…SMB cells and peripheral blood leukocytes were isolated as described previously (33). Briefly, the supraneural myeloid body tissues were stripped from lampreys followed by digestion by 0.1% type 1 collagenase (Sigma-Aldrich) at 4°C overnight.…”

Section: Animals Maintenance and Cell Culturementioning

confidence: 99%

Molecular Evolution of Transforming Growth Factor-β (TGF-β) Gene Family and the Functional Characterization of Lamprey TGF-β2

et al. 2022

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The transforming growth factor-βs (TGF-βs) are multifunctional cytokines capable of regulating a wide range of cellular behaviors and play a key role in maintaining the homeostasis of the immune system. The TGF-β subfamily, which is only present in deuterostomes, expands from a single gene in invertebrates to multiple members in jawed vertebrates. However, the evolutionary processes of the TGF-β subfamily in vertebrates still lack sufficient elucidation. In this study, the TGF-β homologs are identified at the genome-wide level in the reissner lamprey (Lethenteron reissneri), the sea lamprey (Petromyzon marinus), and the Japanese lamprey (Lampetra japonica), which are the extant representatives of jawless vertebrates with a history of more than 350 million years. The molecular evolutionary analyses reveal that the lamprey TGF-β subfamily contains two members representing ancestors of TGF-β2 and 3 in vertebrates, respectively, but TGF-β1 is absent. The transcriptional expression patterns show that the lamprey TGF-β2 may play a central regulatory role in the innate immune response of the lamprey since it exhibits a more rapid and significant upregulation of expression than TGF-β3 during lipopolysaccharide stimuli. The incorporation of BrdU assay reveals that the lamprey TGF-β2 recombinant protein exerts the bipolar regulation on the proliferation of the supraneural myeloid body cells (SMB cells) in the quiescent and LPS-activated state, while plays an inhibitory role in the proliferation of quiescent and activated leukocytes in lampreys. Furthermore, caspase-3/7 activity analysis indicates that the lamprey TGF-β2 protects SMB cells from apoptosis after serum deprivation, in contrast to promoting apoptosis of leukocytes. Our composite results offer valuable clues to the origin and evolution of the TGF-β subfamily and imply that TGF-βs are among the most ancestral immune regulators in vertebrates.

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“…They exist in numerous bodily fluids, including serum, saliva, urine, cerebrospinal fluid, milk, and tears, under both normal and pathological conditions (10,11). Exosomes are packed with various components and have the capacity of inducing functional responses in recipient cells (12)(13)(14)(15). Through the transfer of bioactive molecules between cells, exosomes mediate intercellular signaling and participate in various physiological and pathological processes (16).…”

Section: Introductionmentioning

confidence: 99%

Recent Advances in the Use of Exosomes in Sjögren’s Syndrome

Huang

et al. 2020

Front. Immunol.

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Sjögren’s syndrome (SS) is a chronic autoimmune disorder of the exocrine glands mediated by lymphocytic infiltrates damaging the body tissues and affecting the life quality of patients. Although traditional methods of diagnosis and treatment for SS are effective, in the time of personalized medicine, new biomarkers, and novel approaches are required for the detection and treatment of SS. Exosomes represent an emerging field in the discovery of biomarkers and the management of SS. Exosomes, a subtype of extracellular vesicles, are secreted by various cell types and can be found in most bodily fluids. Exosomes are packed with cytokines and other proteins, bioactive lipids, and nucleic acids (mRNA, circular RNA, non-coding RNA, tRNA, microRNA, genomic DNA, and ssDNA), and transport such cargo between cells. Evidence has indicated that exosomes may play roles in processes such as the modulation of the immune response and activation of inflammation. Moreover, due to features such as stability, low immunogenicity and toxicity, long half-life, and the capacity to penetrate the blood-brain barrier, exosomes have also emerged as therapeutic tools for SS. In this review, we summarize existing literature regarding the biogenesis, isolation, and function of exosomes, specifically focusing on exosomes as novel biomarkers and their potential therapeutic uses in SS.

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Adaptive immune stimuli altered the cargo proteins of exosomes released by supraneural myeloid body cells in Lampetra japonica

Cited by 5 publications

References 51 publications

Post-translational protein deimination signatures in sea lamprey (Petromyzon marinus) plasma and plasma-extracellular vesicles

Post-translational protein deimination signatures in sea lamprey (Petromyzon marinus) plasma and plasma-extracellular vesicles

Molecular Evolution of Transforming Growth Factor-β (TGF-β) Gene Family and the Functional Characterization of Lamprey TGF-β2

Recent Advances in the Use of Exosomes in Sjögren’s Syndrome

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