Adaptive immune response to Edwardsiella tarda infection in ginbuna crucian carp, Carassius auratus langsdorfii

Yamasaki, Masatoshi; Araki, Kyosuke; Nakanishi, Teruyuki; Nakayasu, Chihaya; Yoshiura, Yasutoshi; Iida, Takaji; Yamamoto, Atsushi

doi:10.1016/j.vetimm.2013.02.004

Cited by 37 publications

(15 citation statements)

References 62 publications

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“…These results demonstrate that the mAbs were highly specific to the S. schlegeli IgM and could therefore be used as tools to detect soluble and membrane bound IgM molecules. The highest levels of specific antibodies and sIgM + lymphocytes were both observed at 35 days after challenge with E. tarda, which were consistent with previous reports (Tang et al, 2010;Yamasaki et al, 2013), suggesting that the amount of sIgM + has a close relationship with the antibody titre and the highest level of sIgM + lymphocytes might contribute the peak level of antibody.…”

supporting

confidence: 92%

“…The highest levels of specific antibodies and sIgM + lymphocytes were both observed at 35 days after challenge with E. tarda , which were consistent with previous reports (Tang et al ., ; Yamasaki et al ., ), suggesting that the amount of sIgM + has a close relationship with the antibody titre and the highest level of sIgM + lymphocytes might contribute the peak level of antibody.…”

mentioning

confidence: 99%

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Development and application of monoclonal antibodies against IgM of black rockfish Sebastes schlegeli

Zhang

Tang

Sheng

et al. 2017

Journal of Fish Biology

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Monoclonal antibodies (mAbs) against black rockfish Sebastes schlegeli serum immunoglobulin M (IgM) were developed, which showed a specific reaction with the heavy chain of S. schlegeli IgM in Western blotting and with surface IgM positive (sIgM ) lymphocytes in indirect immunofluorescence. mAb 2A6 was employed to investigate the antibody and sIgM lymphocyte responses of S. schlegeli injected with inactivated Edwardsiella tarda, by ELISA and flow cytometry. Compared with controls, the level of specific antibodies and the percentage of sIgM lymphocytes both increased in the immunized fish and simultaneously reached their peaks at day 35 after immunization.

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supporting

confidence: 92%

mentioning

confidence: 99%

Development and application of monoclonal antibodies against IgM of black rockfish Sebastes schlegeli

Zhang

Tang

Sheng

et al. 2017

Journal of Fish Biology

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“…Recently, with the production of specific antibodies for fish T lymphocytes, it has become possible to investigate cellular immune responses at the translational level. This has been exemplified by the investigation of cellular immune responses induced by various antigens in carp and rainbow trout (54)(55)(56)(57)(58)(59). In this study, we used FCM analysis of single stained CD4-1 + , CD4-2 + , and CD8β + cells to evaluate the cellular immune responses induced by the pVAA vaccine.…”

Section: Discussionmentioning

confidence: 99%

A DNA Vaccine Encoding the VAA Gene of Vibrio anguillarum Induces a Protective Immune Response in Flounder

Xing

Tang

et al. 2019

Front. Immunol.

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Vibrio anguillarum is a pathogenic bacterium that infects flounder resulting in significant losses in the aquaculture industry. The VAA protein previously identified in flounder is associated with a role in immune protection within these fish. In the present study, a recombinant DNA plasmid encoding the VAA gene of V. anguillarum was constructed and its potential as a DNA vaccine, to prevent the infection of V. anguillarum in flounder fish, investigated. We verified the expression of the VAA protein both in vitro in cell lines and in vivo in flounder fish. The protective effects of pcDNA3.1-VAA (pVAA) were analyzed by determination of the percentage of sIgM+, CD4-1+, CD4-2+, CD8β+ lymphocytes, and the production of VAA-specific antibodies in flounder following their immunization with the DNA vaccine. Histopathological changes in immune related tissues, bacterial load, and relative percentage survival rates of flounder post-challenge with V. anguillarum, were all investigated to assess the efficacy of the pVAA DNA vaccine candidate. Fish intramuscularly immunized with pVAA showed a significant increase in CD4-1+, CD4-2+, and CD8β+ T lymphocytes at days 9, 11, and 14 post-vaccination, reaching peak T-cell levels at days 11 or 14 post-immunization. The percentage of sIgM+ lymphocytes reached peak levels at weeks 4–5 post-immunization. Specific anti-V. anguillarum or anti-rVAA antibodies were induced in inoculated fish at days 28–35 post-immunization. The liver of vaccinated flounder exhibited only slight histopathological changes compared with a significant pathology observed in control immunized fish. Additionally, a lower bacterial burden in the liver, spleen, and kidney were observed in pVAA protected fish in response to bacterial challenge, compared with pcDNA3.1 vector control injected fish. Moreover, the pVAA vaccine confers a relative percentage survival of 50.00% following V. anguillarum infection. In summary, this is the first study indicating an initial induction of the T lymphocyte response, followed by B lymphocyte induction of specific antibodies as a result of DNA immunization of flounder. This signifies the important potential of pVAA as a DNA vaccine candidate for the control of V. anguillarum infection.

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“…The microbiological diagnoses on freshly dead fish (in both the treated and the untreated groups) in the in vivo test revealed two opportunistic pathogens C. freundii and E. tarda. The clinical signs observed in the fish have previously been described in infections caused by both of these bacteria (T€ urk et al 2013;Yamasaki et al 2013).…”

Section: Discussionmentioning

confidence: 76%

Essential oils to control ichthyophthiriasis in pacu, Piaractus mesopotamicus (Holmberg): special emphasis on treatment with Melaleuca alternifolia

Valladão

Gallani

Ikefuti

et al. 2016

Journal of Fish Diseases

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In vitro effect of the Melaleuca alternifolia, Lavandula angustifolia and Mentha piperita essential oils (EOs) against Ichthyophthirius multifiliis and in vivo effect of M. alternifolia for treating ichthyophthiriasis in one of the most important South American fish, Piaractus mesopotamicus (Holmberg), were evaluated. The in vitro test consisted of three EOs, each at concentrations of 57 μL L(-1) , 114 μL L (-1) , 227 μL L(-1) and 455 μL L (-1) , which were assessed once an hour for 4 h in microtitre plates (96 wells). The in vitro results demonstrated that all tested EOs showed a cytotoxic effect against I. multifiliis compared to control groups (P < 0.05). The in vivo treatment for white spot disease was performed in a bath for 2 h day(-1) for 5 days using the M. alternifolia EO (50 μL L (-1) ). In this study, 53.33% of the fish severely infected by I. multifiliis survived after the treatment with M. alternifolia (50 μL L (-1) ) and the parasitological analysis has shown an efficacy of nearly 100% in the skin and gills, while all the fish in the control group died. Furthermore, the potential positive effect of M. alternifolia EO against two emergent opportunistic bacteria in South America Edwardsiella tarda and Citrobacter freundii was discussed.

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Adaptive immune response to Edwardsiella tarda infection in ginbuna crucian carp, Carassius auratus langsdorfii

Cited by 37 publications

References 62 publications

Development and application of monoclonal antibodies against IgM of black rockfish Sebastes schlegeli

Development and application of monoclonal antibodies against IgM of black rockfish Sebastes schlegeli

A DNA Vaccine Encoding the VAA Gene of Vibrio anguillarum Induces a Protective Immune Response in Flounder

Essential oils to control ichthyophthiriasis in pacu, Piaractus mesopotamicus (Holmberg): special emphasis on treatment with Melaleuca alternifolia

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