Adaptive gene profiling of Mycobacterium tuberculosis during sub-lethal kanamycin exposure

Habib, Zeshan; Xu, Weize; Jamal, Muhammad; Rehman, Khaista; Dai, Jinxia; Fu, Zhen F.; Chen, Xi; Cao, Gang

doi:10.1016/j.micpath.2017.09.055

Cited by 5 publications

(4 citation statements)

References 68 publications

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“…The most recent study of the transcriptional response to antibiotics in Mycobacterium concerns the response following sublethal kanamycin exposure (Habib et al, 2017). Kanamycin is a second-line drug used for the treatment of patients with MDR-TB.…”

Section: Transcriptional Signature and Drug Mode Of Actionmentioning

confidence: 99%

Genome-Wide Transcriptional Responses of Mycobacterium to Antibiotics

2019

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Antibiotics can stimulate or depress gene expression in bacteria. The analysis of transcriptional responses of Mycobacterium to antimycobacterial compounds has improved our understanding of the mode of action of various drug classes and the efficacy and effect of such compounds on the global metabolism of Mycobacterium . This approach can provide new insights for known antibiotics, for example those currently used for tuberculosis treatment, as well as help to identify the mode of action and predict the targets of new compounds identified by whole-cell screening assays. In addition, changes in gene expression profiles after antimycobacterial treatment can provide information about the adaptive ability of bacteria to escape the effects of antibiotics and allow monitoring of the physiology of the bacteria during treatment. Genome-wide expression profiling also makes it possible to pinpoint genes differentially expressed between drug sensitive Mycobacterium and multidrug-resistant clinical isolates. Finally, genes involved in adaptive responses and drug tolerance could become new targets for improving the efficacy of existing antibiotics.

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Section: Transcriptional Signature and Drug Mode Of Actionmentioning

confidence: 99%

Genome-Wide Transcriptional Responses of Mycobacterium to Antibiotics

2019

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“…In addition, both studies have identified DEGs that are involved in biological process including ribosome and translation, TCA cycle, glycolysis or carbohydrate metabolism (Supplementary Tables S2, S6; Zhou et al, 2019). Genome-wide transcriptome profiling of Mycobacterium tuberculosis upon treatment with Kanamycin has been reported (Habib et al, 2017). Comparison with the functional classification and pathways of the DEGs identified in the M. tuberculosis study with those identified in this study in E. coli indicated more differences than similarities between the two studies.…”

Section: Discussionmentioning

confidence: 62%

“…Genome-wide transcriptome profiling of Mycobacterium tuberculosis upon treatment with Kanamycin has been reported ( Habib et al, 2017 ). Comparison with the functional classification and pathways of the DEGs identified in the M. tuberculosis study with those identified in this study in E. coli indicated more differences than similarities between the two studies.…”

Section: Discussionmentioning

confidence: 99%

“…RNA-Seq analysis of Pseudomonas aeruginosa swarming cells at subinhibitory concentrations of tobramycin identified expression changes in gene encoding multidrug efflux pump and virulence factors ( Coleman et al, 2020 ). In addition, transcriptional profiling changes in Mycobacterium tuberculosis in response to Kanamycin have been observed ( Habib et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

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Transcriptome profiling in response to Kanamycin B reveals its wider non-antibiotic cellular function in Escherichia coli

et al. 2022

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Aminoglycosides are not only antibiotics but also have wider and diverse non-antibiotic cellular functions. To elucidate the understanding of non-antibiotic cellular functions, here we report transcriptome-profiling analysis of Escherichia coli in the absence or presence of 0.5 and 1 μM of Kanamycin B, concentrations that are neither lethal nor inhibit growth, and identified the differentially expressed genes (DEGs) at two given concentrations of Kanamycin B. Functional classification of the DEGs revealed that they were mainly related to microbial metabolism including two-component systems, biofilm formation, oxidative phosphorylation and nitrogen metabolism in diverse environments. We further showed that Kanamycin B and other aminoglycosides can induce reporter gene expression through the 5′ UTR of napF gene or narK gene (both identified as DEG) and Kanamycin B can directly bind to the RNA. The results provide new insights into a better understanding of the wider aminoglycosides cellular function in E. coli rather than its known antibiotics function.

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