Adaptive changes induced by chronic ethanol ingestion on hepatic mitochondrial and microsomal enzyme activities

Sanchez-Amate, M.C.; Carrasco, Marı́a P.; Marco, Carmen; Segovia, J.L.

doi:10.1016/1357-2725(95)00118-2

Cited by 5 publications

(2 citation statements)

References 16 publications

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“…Since PS synthase is a membrane-bound enzyme and membranes are the main target of ethanol this differential action indicates that chronic ethanol ingestion alters the membranes, thus producing cells that are resistant to re-exposure to ethanol in vitro. This phenomenon of cell adaptation to ethanol, known as tolerance, has been previously demonstrated for other activities in our [16] and other laboratories [24].…”

Section: Resultsmentioning

confidence: 93%

“…All the evidence indicates that chronic ethanol ingestion causes profound structural, compositional and functional alterations to cells, subcellular organelles and membranes [16][17][18], although we still do not understand the precise pattern of molecular modification nor exactly how the cell mechanism is altered. Furthermore, despite the fact that it is widely shown in vitro that the consumption of ethanol gives rise to membranes resistant to its disordering effect [19,20], there is little information available concerning adaptative mechanisms to ethanol at cell level.…”

Section: Resultsmentioning

confidence: 99%

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Chronic ingestion of ethanol stimulates lipogenic response in rat hepatocytes

2001

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We isolated hepatocytes from rats chronically fed with ethanol and pair-fed control rats and incubated them both in the presence and absence of 100 mM ethanol in order to analyze the uptake into their lipids of several radiolabeled exogenous substrates. The hepatocytes treated chronically with ethanol showed higher lipogenic activity both in neutral lipids and phospholipids from serine, ethanolamine, glycerol and oleate. The only exception found was in the incorporation of choline into phosphatidylcholine (PC), which was lower in the hepatocytes from ethanol-fed rats than in the controls and was concomitant with a decrease in the PC levels of the ethanol-fed hepatocytes. The results obtained after exposing the cells to 100 mM ethanol in vitro indicate that in general the hepatocytes from ethanol-fed rats exhibit a higher lipogenic activity than the control cells. The only difference in the response to ethanol in vitro was found in the biosynthesis of phosphatidylserine (PS) from serine, which rose significantly in control cells but was unaffected in alcoholic hepatocytes. We put this difference in response down to specific adaptation to ethanol feeding.

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Section: Resultsmentioning

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Chronic ingestion of ethanol stimulates lipogenic response in rat hepatocytes

2001

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Effects of ethanol and dimethylsulphoxide on nuclear and cytoplasmic maturation of bovine cumulus-oocyte complexes

Avery¹,

Greve²

2000

Mol. Reprod. Dev.

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Hormetic acute response and chronic effect of ethanol on adenine nucleotide hydrolysis in rat platelets

et al. 2008

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The objective of this study was to verify the acute and chronic effects of ethanol on platelet NTPDase and 5'-nucleotidase activities. These enzymes modulate platelet function by regulating adenine nucleotide bioavailability and adenosine production. In the acute treatment, doses of 0.8, 2.0, 4.0, 6.0 and 8.0 g/kg ethanol were administered via orogastric tube, and induced a biphasic or hormetic effect on ATP, ADP and AMP platelet hydrolysis. Ethanol at a dose of 0.8 and 2.0 g/kg increased NTPDase activity (44 and 35%, P < 0.0001) with ATP as substrate, whereas when ADP was used there was only a tendency for NTPDase activity to increase. ATP and ADP hydrolysis decreased by 31-77% (P < 0.0001) in 4.0, 6.0 and 8.0 g/kg of ethanol compared to the control. AMP hydrolysis showed a tendency to increase at ethanol doses of 0.8 and 2.0 g/kg, but was inhibited by 45-100% (P < 0.0001) at the higher doses. Chronic treatment consisted of the oral administration of 20% ethanol solution during 31 weeks as the only source of liquid and inhibited NTPDase activity (15 and 20%, P < 0.05) with ATP and ADP as substrate, respectively. However, AMP hydrolysis by 5'-nucleotidase increased by 40% (P < 0.05). Thus, we speculate that the effects of ethanol on NTPDase and 5'-nucleotidase activities could be related with the platelets alterations commonly observed in alcohol users.

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Adaptive changes induced by chronic ethanol ingestion on hepatic mitochondrial and microsomal enzyme activities

Cited by 5 publications

References 16 publications

Chronic ingestion of ethanol stimulates lipogenic response in rat hepatocytes

Chronic ingestion of ethanol stimulates lipogenic response in rat hepatocytes

Effects of ethanol and dimethylsulphoxide on nuclear and cytoplasmic maturation of bovine cumulus-oocyte complexes

Hormetic acute response and chronic effect of ethanol on adenine nucleotide hydrolysis in rat platelets

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