Adaptive aberration correction of GRIN lenses for confocal endomicroscopy

Lee, W. M.; Yun, Seok Hyun

doi:10.1364/ol.36.004608

Cited by 44 publications

(33 citation statements)

References 14 publications

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“…Even though the combination of GRIN lenses with two-photon imaging benefits of improved optical sectioning, most GRIN endoscopes have been operating with lower resolution even in twophoton excitation modality due to optical aberrations lying on and off the optical axis. These aberrations derive from the intrinsic nonaplanatic properties of GRIN rods (45) . To correct off-axis aberrations while maintaining high NA, at least a second planoconvex lens was needed (21).…”

Section: Resultsmentioning

confidence: 99%

Extended field-of-view ultrathin microendoscopes with built-in aberration correction for high-resolution imaging with minimal invasiveness

Antonini

Bovetti

Moretti

et al. 2018

Preprint

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We present a novel approach to correct optical aberrations in ultrathin gradient-index rod lens-based endoscopes using microfabricated aspherical lenses. Corrected microendoscopes have up to 9 folds larger field-of-view compared to uncorrected probes. Using extended field-of-view (eFOV) microendoscopes, we report two-photon imaging of GCaMP6 signals in the mouse hippocampus in vivo with unprecedented combination of high spatiotemporal resolution and minimal invasiveness.

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Section: Resultsmentioning

confidence: 99%

Extended field-of-view ultrathin microendoscopes with built-in aberration correction for high-resolution imaging with minimal invasiveness

Antonini

Bovetti

Moretti

et al. 2018

Preprint

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“…Even though the combination of GRIN lenses with two-photon imaging allows improved optical sectioning, most GRIN endoscopes have been operating with limited performance due to optical aberrations. These aberrations derive from the intrinsic nonaplanatic properties of GRIN rods 56 and limit the spatial resolution and the usable FOV 25,28,30,32 . Aberrations typically increase with the length and NA of the GRIN lens, making high-resolution imaging of large FOV in deeper areas a challenging goal.…”

Section: Discussionmentioning

confidence: 99%

“…2). Aberration correction in GRIN microendoscopes can be achieved using adaptive optics (AO) 28,30,31,56 . For example, using pupilsegmentation methods for AO, diffraction-limited performance across an enlarged FOV was obtained in GRIN-based endoscopes with diameter of 1.4 mm 28,30 and, in principle, this approach could be extended to probes with smaller diameter.…”

Section: Discussionmentioning

confidence: 99%

Extended field-of-view ultrathin microendoscopes for high-resolution two-photon imaging with minimal invasiveness in awake mice

Antonini

Sattin

Moroni

et al. 2020

Preprint

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Imaging neuronal activity with high and homogeneous spatial resolution across the field-of-view (FOV) and limited invasiveness in deep brain regions is fundamental for the progress of neuroscience, yet is a major technical challenge. We achieved this goal by correcting optical aberrations in gradient index lens-based ultrathin (≤ 500 µm) microendoscopes using aspheric microlenses generated through 3D-microprinting. Corrected microendoscopes had extended FOV (eFOV) with homogeneous spatial resolution for two-photon fluorescence imaging and required no modification of the optical set-up. Synthetic calcium imaging data showed that, compared to uncorrected endoscopes, eFOV-microendoscopes led to improved signal-to-noise ratio and more precise evaluation of correlated neuronal activity. We experimentally validated these predictions in awake head-fixed mice. Moreover, using eFOV-microendoscopes we demonstrated cell-specific encoding of behavioral state-dependent information in distributed functional subnetworks in a primary somatosensory thalamic nucleus. eFOV-microendoscopes are, therefore, small-crosssection ready-to-use tools for deep two-photon functional imaging with unprecedentedly high and homogeneous spatial resolution.

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“…Adaptive optics has therefore been introduced to overcome these problems and enable high quality imaging through these imperfect optical endoscope systems. [56][57][58] Spatial light modulators have also been used in fiber bundle endoscopes to implement beam scanning with the potential for further aberration correction. 59 Stimulated emission depletion (STED) microscopes STED microscopes create images with resolution beyond the diffraction limit by employing a combination of optical and photophysical effects.…”

Section: Light Sheet Microscopesmentioning

confidence: 99%

Adaptive optical microscopy: the ongoing quest for a perfect image

2014

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Adaptive optics is becoming a valuable tool for high resolution microscopy, providing correction for aberrations introduced by the refractive index structure of specimens. This is proving particularly promising for applications that require images from deep within biological tissue specimens. We review recent developments in adaptive microscopy, including methods and applications. A range of advances in different microscope modalities is covered and prospects for the future are discussed. INTRODUCTIONOptical microscopes have for a long time played a vital role in biomedical research. Their ability to provide multidimensional structural and functional information about a specimen in a non-invasive manner has permitted numerous scientific advances. These microscopes rely upon high-quality optical systems to operate at the optimum resolution, which is determined by the diffraction limit of light for conventional microscopes. However, even with perfect optical components, the performance of the microscope is affected by the optical properties of the specimen. Spatial variations in refractive index introduce aberrations as the light passes through the specimen-a problem that is exacerbated when imaging deeps into tissue. These aberrations detrimentally affect the resolution and contrast of microscope images, ultimately limiting the depth at which imaging is practical. Adaptive optics (AO) was introduced into microscopy in order to overcome this limitation. Dynamic correction elements, such as deformable mirrors (DMs) or spatial light modulators (SLMs) have been employed to compensate specimen-induced aberrations (Figure 1). New wavefront sensing and control schemes have been developed to adapt the correction elements.The first demonstrations of adaptive optical microscopy took place in the early 2000s. In the few years that followed, there were several innovative advances that set the scene for further research. The research in this period is well covered by two review articles 1,2 and a recent compiled volume, 3 so this will not be reproduced here. This present article provides a review of advances in aberration correction in adaptive microscopy that have taken place in the last half-decade. During this period, a wide range of AO techniques have emerged, which have considerably enhanced the available toolkit. Furthermore, the benefit of correction of specimen-induced aberrations has been shown in different areas, particularly (although not exclusively) in biomedical applications. We outline these technological

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Adaptive aberration correction of GRIN lenses for confocal endomicroscopy

Cited by 44 publications

References 14 publications

Extended field-of-view ultrathin microendoscopes with built-in aberration correction for high-resolution imaging with minimal invasiveness

Extended field-of-view ultrathin microendoscopes with built-in aberration correction for high-resolution imaging with minimal invasiveness

Extended field-of-view ultrathin microendoscopes for high-resolution two-photon imaging with minimal invasiveness in awake mice

Adaptive optical microscopy: the ongoing quest for a perfect image

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