Adapting a porcine reproductive and respiratory syndrome virus (PRRSV) oral fluid antibody ELISA to routine surveillance

Henao-Díaz, Alexandra; Zhang, Min; Giménez-Lirola, Luis; E, Ramirez; Gauger, Phillip C.; Baum, David H.; Clavijo, Maria J.; Rotolo, Marisa; Main, Rodger; Zimmerman, Jeffrey J.

doi:10.1016/j.prevetmed.2020.105250

Cited by 2 publications

(1 citation statement)

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“…Another explanation could be that the positive ELISA results of the samples taken at week 11 in batch 2 represented false positive results. Indeed, a previous study revealed that certain animal husbandry or production practices may be associated with non-speci c reactions in the ELISA kit used in our study (9). To increase the speci city of the test, they suggested to use a higher cut-off of 1.0 S/P value in contrast to 0.4 which is recommended by the vendor and used in our study.…”

Section: Discussionmentioning

confidence: 96%

Impact of weaning procedures on Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) circulation in the nursery section

Heiselberg,

Kristensen,

Kvisgaard

et al. 2023

Preprint

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The impact of different weaning strategies on the downstream circulation of PRRSV has not been widely described. It is, however, believed that mixing pigs of different age groups is increasing the risk of PRRSV circulation in the nursery section. In this study, pigs were sampled in three herds that performed “mixed at weaning (MIX)” and three herds that performed “all in/all out at weaning (AIAO)”. MIX included holding underweighted piglets back in containers for two weeks and then move them to nursery facilities with newly weaned piglets from subsequent batches. Oral fluid samples were collected from four batches of pigs in each herd, three times from weaning until 30kg for each batch, and tested for PRRSV and PRRSV antibodies. Herds that performed MIX at weaning had an eightfold increase in risk of detecting PRRSV in oral fluids compared to herds with AIAO. In total, 41 oral fluid samples from eight batches in MIX herds and five oral fluid samples from two batches in AIAO herds tested positive for PRRSV. The titer of PRRSV-antibodies in oral fluid samples from weaners decreased in most of the batches in the AIAO herds and increased in most MIX herds. In addition to oral fluids, tongue tip samples were collected from dead pigs and tested for PRRSV. In 17 of 23 batches the results of the tongue tip samples correlated with the results of the oral fluid samples (κ = 0.44) indicating a good agreement between the two materials for sampling. Overall, the results of the study confirmed that the weaning strategy had a significant impact on the circulation of PRRSV post weaning.

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Section: Discussionmentioning

confidence: 96%