Adapter-modified Ussing chamber enables evaluation of endoscopically-obtained colonic biopsy samples from cats and dogs

Ruhnke, Isabelle; DeBiasio, John V.; Suchodolski, Jan S.; Newman, Shelley J.; Musch, Mark W.; Steiner, Jörg M.

doi:10.1016/j.rvsc.2012.05.009

Cited by 3 publications

(3 citation statements)

References 31 publications

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“…1) was used for this study [31]. The centrally placed adapter disc enables investigation of tissue samples covering a size of 5 mm 2 , which is the approximate size of endoscopically collected intestinal biopsy samples (eMachineShop, USA).…”

Section: Methodsmentioning

confidence: 99%

“…Evaluation of intestinal transport physiology with a Ussing chamber might also be beneficial as a research and/or diagnostic tool for gastrointestinal disorders in dogs and/or cats. We previously demonstrated that an adapter-modified Ussing chamber could be used for evaluation of transport physiology of colonic biopsies [31]. The present study was conducted to investigate the use of the adapter-modified Ussing chamber for evaluation of intestinal mucosal transport in endoscopically collected biopsies from the duodenum of dogs and cats, as well as to assess duodenal transport physiology of healthy dogs and dogs with gastrointestinal diseases using this technology.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of endoscopically obtained duodenal biopsy samples from cats and dogs in an adapter-modified Ussing chamber

et al. 2014

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This study was conducted to evaluate an adapter-modified Ussing chamber for assessment of transport physiology in endoscopically obtained duodenal biopsies from healthy cats and dogs, as well as dogs with chronic enteropathies. 17 duodenal biopsies from five cats and 51 duodenal biopsies from 13 dogs were obtained. Samples were transferred into an adapter-modified Ussing chamber and sequentially exposed to various absorbagogues and secretagogues. Overall, 78.6% of duodenal samples obtained from cats responded to at least one compound. In duodenal biopsies obtained from dogs, the rate of overall response ranged from 87.5% (healthy individuals; n = 8), to 63.6% (animals exhibiting clinical signs of gastrointestinal disease and histopathological unremarkable duodenum; n = 15), and 32.1% (animals exhibiting clinical signs of gastrointestinal diseases and moderate to severe histopathological lesions; n = 28). Detailed information regarding the magnitude and duration of the response are provided. The adapter-modified Ussing chamber enables investigation of the absorptive and secretory capacity of endoscopically obtained duodenal biopsies from cats and dogs and has the potential to become a valuable research tool. The response of samples was correlated with histopathological findings.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Evaluation of endoscopically obtained duodenal biopsy samples from cats and dogs in an adapter-modified Ussing chamber

et al. 2014

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“…Ussing chambers have been used extensively to study nutrient absorption across gut epithelial tissues for a range of different animal species, including rats and pigs. Ussing chambers have been used to study the uptake of glucose, heparin, oligonucleotides, antibiotics and amino acids (7,20,(27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39)(40)(41). The Ussing chamber is an ex vivo technique in which gut tissue is collected and mounted between two buffer-containing reservoirs (luminal and serosal chambers), thus permitting the study of the absorption of compounds across mounted tissues (28)(29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39)(40)(41)(42)(43)(44).…”

Section: Studies On Materials Transport Across a Gastrointestinal Tra...mentioning

confidence: 99%

Use of the Ussing chamber technique to study nutrient transport by epithelial tissues

Ye²,

Li³

et al. 2013

Front Biosci

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The Ussing chamber provides a physiologically relevant system for measuring the transport of ions, nutrients, and drugs across various epithelial tissues. This article outlines the design, structure, principle, and operation of the Ussing chamber, its application in the field of gastrointestinal barrier function and nutrient transport research, as well as its advantages and limitations. This review serves as a practical guide for investigators who are new to the Ussing chamber and should help researchers better understand this valuable method for measuring the transport of electrolytes, organic nutrients, water, and drugs across the small intestine, placenta, and other epithelial tissues.

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Physiologic Factors Related to Drug Absorption

Acharya

Fernandes

Mallik

et al. 2018

Dosage Form Design Considerations

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Adapter-modified Ussing chamber enables evaluation of endoscopically-obtained colonic biopsy samples from cats and dogs

Cited by 3 publications

References 31 publications

Evaluation of endoscopically obtained duodenal biopsy samples from cats and dogs in an adapter-modified Ussing chamber

Evaluation of endoscopically obtained duodenal biopsy samples from cats and dogs in an adapter-modified Ussing chamber

Use of the Ussing chamber technique to study nutrient transport by epithelial tissues

Physiologic Factors Related to Drug Absorption

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