Adaptations to Environmental Stresses

Bohnert, Hans J.; Nelson, Donald E.; Jensen, Richard G.

doi:10.2307/3870060

Cited by 683 publications

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“…The sequences of the PINO1 gene have been deposited in GenBank TM under accession number AF412340. 2 Complementation of RINO1 and PINO1 in Yeast Inositol Auxotrophic Strain FY250 -The procedure followed was that of Bacchawat and Mande (9). PCR-amplified RINO1 and PINO1 genes were cloned into pGEMT-T easy vector and were subsequently cloned downstream of a strong constitutively expressed yeast glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter of a yeast multicopy expression vector p426 GAPDH within EcoRI/XhoI restriction sites.…”

Section: Methodsmentioning

confidence: 99%

“…As phosphorylated derivatives, its role as a phosphorus store and as a "second messenger" in signal transduction pathways has long been recognized. myo-Inositol, physiologically the most favored stereoisomer among the eight possible geometric isomers of inositol, also enters into an array of biochemical reactions having diverse functions in cellular metabolism both as free and conjugated and phosphorylated or methylated forms (1)(2)(3).…”

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confidence: 99%

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A Novel Salt-tolerant l-myo-Inositol-1-phosphate Synthase from Porteresia coarctata (Roxb.) Tateoka, a Halophytic Wild Rice

Majee

Maitra

Dastidar

et al. 2004

Journal of Biological Chemistry

168

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L-myo-Inositol-1-phosphate synthase (EC 5.5.1.4, MIPS), an evolutionarily conserved enzyme protein, catalyzes the synthesis of inositol, which is implicated in a number of metabolic reactions in the biological kingdom. Here we report on the isolation of the gene (PINO1) for a novel salt-tolerant MIPS from the wild halophytic rice, Porteresia coarctata (Roxb.) Tateoka. Identity of the PINO1 gene was confirmed by functional complementation in a yeast inositol auxotrophic strain. Comparison of the nucleotide and deduced amino acid sequences of PINO1 with that of the homologous gene from Oryza sativa L. (RINO1) revealed distinct differences in a stretch of 37 amino acids, between amino acids 174 and 210. Purified bacterially expressed PINO1 protein demonstrated a salt-tolerant character in vitro compared with the salt-sensitive RINO1 protein as with those purified from the native source or an expressed salt-sensitive mutant PINO1 protein wherein amino acids 174 -210 have been deleted. Analysis of the salt effect on oligomerization and tryptophan fluorescence of the RINO1 and PINO1 proteins revealed that the structure of PINO1 protein is stable toward salt environment. Furthermore, introgression of PINO1 rendered transgenic tobacco plants capable of growth in 200 -300 mM NaCl with retention of ϳ40 -80% of the photosynthetic competence with concomitant increased inositol production compared with unstressed control. MIPS protein isolated from PINO1 transgenics showed salt-tolerant property in vitro confirming functional expression in planta of the PINO1 gene. To our knowledge, this is the first report of a salt-tolerant MIPS from any source.Inositols are six-carbon cyclohexane hexitols found ubiquitously in the biological kingdom, and its metabolism plays a vital role in growth regulation, membrane biogenesis, osmotolerance, and in many other processes. As phosphorylated derivatives, its role as a phosphorus store and as a "second messenger" in signal transduction pathways has long been recognized. myo-Inositol, physiologically the most favored stereoisomer among the eight possible geometric isomers of inositol, also enters into an array of biochemical reactions having diverse functions in cellular metabolism both as free and conjugated and phosphorylated or methylated forms (1-3).The primary enzyme for the synthesis of L-myo-inositol 1-phosphate from glucose 6-phosphate is L-myo-inositol-1-phosphate synthase (EC 5.5.1.4; referred to as MIPS), 1 which synthesizes L-myo-inositol 1-phosphate through an internal oxidoreduction reaction involving NAD ϩ . Free inositol is generated by dephosphorylation of the MIPS product by a specific Mg 2ϩ -dependent inositol-1-phosphate phosphatase (EC 3.1.3.25). This mechanism is followed by all myo-inositolproducing organisms throughout the phylogenetic lines, and MIPS has been identified as an evolutionarily conserved protein (4). The structural gene coding for cytosolic MIPS, termed INO1, was first identified in yeast, Saccharomyces cerevisiae (5, 6) and cloned by Klig and Henry (7)....

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

A Novel Salt-tolerant l-myo-Inositol-1-phosphate Synthase from Porteresia coarctata (Roxb.) Tateoka, a Halophytic Wild Rice

Majee

Maitra

Dastidar

et al. 2004

Journal of Biological Chemistry

168

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“…These abiotic stress factors generate secondary stresses, i.e. osmotic and oxidative stress, which have negative influence on the plant, causing changes in its normal growth, development and metabolism (Bohenert et al 1995;Kranner et al 2010). Thus, it is necessary to conduct research focused on obtaining cultivars, which would be more tolerant to adverse environmental factors.…”

Section: Introductionmentioning

confidence: 99%

Changes in the composition of phenolic compounds and antioxidant properties of grapevine roots and leaves (Vitis vinifera L.) under continuous of long-term drought stress

2014

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Grapevine seedlings Vitis vinifera L. were grown in a greenhouse under optimum conditions (soil moisture ca 70 %) and under drought stress (soil moisture ca 30 %). Drought stress caused reduction in total phenolic compounds in grapevine leaves and roots, where were identified tree phenolic acids: caffeic acid, p-coumaric acid and ferulic acid. All acids found in leaves and roots occurred in the ester-bound form. Only caffeic acid in leaves appeared in the free and ester-bound form. Caffeic acid was present in the highest concentrations. The content of ferulic acid was the lowest in both tissues. The levels of all phenolic acids in leaves and roots decreased significantly under the drought stress. All the extracts from grapevine leaves and roots had antioxidative properties, but the antiradical activity of the extracts obtained from roots subjected to drought stress was lower to the control. The results of the analysis revealed that long-term drought stress caused a decrease in selected elements of secondary metabolism in such a different plant tissues that are the leaves and roots of the grapevine.

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“…Salinity is an abiotic factor that combines elements of water deficit and sodium (Na) toxicity (Bohnert et al 1995). It is considered as one of the most important abiotic stresses that limits crop productivity, affecting several aspects of plant metabolism that generally results in the reduction of plant growth in non-halophytes (Munns & Termaat 1986).…”

Section: Introductionmentioning

confidence: 99%

Growth and physiological characterisation of regenerated potato (Solanum tuberosum) plants affected by NaCI stress

Marconi

Benavides

Caso

2001

New Zealand Journal of Crop and Horticultural Science

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A comparative growth analysis was made between salt-tolerant plants obtained by recurrent selection methods in vitro and Solanum tuberosum L. 'Kennebec' original mother plants (wild type). They were grown at three different salt concentrations (0.5, 25, and 100 mMNaCl) by hydroponic culture. Fourteen days after the saline treatment was applied, no differences in growth were observed between clones. However, 14 days later (Day 28), the comparative analysis between clones revealed intra-especific differences. The salt-tolerant line (clone 150) exhibited greater tolerance to the highest salt doses. Plants of this clone had greater biomass than wild type (clone T) at all salt concentrations tested. The relative growth rate (RGR) and components, as well as the relative accumulation rate of water (RAR water ) and K + and Na + content, should explain, at least in part, the salt-tolerance of clone 150. H00008

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Adaptations to Environmental Stresses

Cited by 683 publications

References 0 publications

A Novel Salt-tolerant l-myo-Inositol-1-phosphate Synthase from Porteresia coarctata (Roxb.) Tateoka, a Halophytic Wild Rice

A Novel Salt-tolerant l-myo-Inositol-1-phosphate Synthase from Porteresia coarctata (Roxb.) Tateoka, a Halophytic Wild Rice

Changes in the composition of phenolic compounds and antioxidant properties of grapevine roots and leaves (Vitis vinifera L.) under continuous of long-term drought stress

Growth and physiological characterisation of regenerated potato (Solanum tuberosum) plants affected by NaCI stress

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