Adaptation Processes in Insect Olfactory Receptors

Kaissling, Karl-Ernst; Strausfeld, Camilla Zack; Rumbo, E. R.

doi:10.1111/j.1749-6632.1987.tb43475.x

Cited by 104 publications

(87 citation statements)

References 15 publications

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“…S2C). This spike adaptation probably resulted from a change in the spike-generating mechanism, such as the inactivation of voltage-gated sodium channels (34). Similar spike adaptation in Ir-expressing OSNs has been shown in one (15), but not in another (35), study via SSR.…”

Section: Patch-clamp Recordings Of Odor Responses In Or-expressing Osnssupporting

confidence: 65%

“…This adaptation may be caused by perineuronal effects, such as the depletion of odorant-binding proteins or ionic concentration changes in sensillar lymph (34), or by desensitized cellular signaling intrinsic to the OSNs. To distinguish between these possibilities, we examined the adaptation of OSNs with their sensory dendrites pulled out of the sensillar cavities to preclude any perineuronal effects.…”

Section: Patch-clamp Recordings Of Odor Responses In Or-expressing Osnsmentioning

confidence: 99%

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Distinct signaling of Drosophila chemoreceptors in olfactory sensory neurons

Cao

Jing

Yang

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

114

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In Drosophila, olfactory sensory neurons (OSNs) rely primarily on two types of chemoreceptors, odorant receptors (Ors) and ionotropic receptors (Irs), to convert odor stimuli into neural activity. The cellular signaling of these receptors in their native OSNs remains unclear because of the difficulty of obtaining intracellular recordings from Drosophila OSNs. Here, we developed an antennal preparation that enabled the first recordings (to our knowledge) from targeted Drosophila OSNs through a patch-clamp technique. We found that brief odor pulses triggered graded inward receptor currents with distinct response kinetics and current-voltage relationships between Or-and Ir-driven responses. When stimulated with long-step odors, the receptor current of Ir-expressing OSNs did not adapt. In contrast, Or-expressing OSNs showed a strong Ca 2+ -dependent adaptation. The adaptation-induced changes in odor sensitivity obeyed the Weber-Fechner relation; however, surprisingly, the incremental sensitivity was reduced at low odor backgrounds but increased at high odor backgrounds. Our model for odor adaptation revealed two opposing effects of adaptation, desensitization and prevention of saturation, in dynamically adjusting odor sensitivity and extending the sensory operating range.Drosophila | olfaction | chemoreceptor | sensory adaptation | OSN

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Section: Patch-clamp Recordings Of Odor Responses In Or-expressing Osnssupporting

confidence: 65%

Section: Patch-clamp Recordings Of Odor Responses In Or-expressing Osnsmentioning

confidence: 99%

Distinct signaling of Drosophila chemoreceptors in olfactory sensory neurons

Cao

Jing

Yang

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

114

View full text Add to dashboard Cite

show abstract

“…The half-times of the rising and declining phases were analyzed to compare our results with studies on silkmoths (Antheraea sp. ; Zack, 1979;Kaissling et al, 1987;Kodadová, 1993;Kodadová and Kaissling, 1996), and the initial slope was analyzed as an important parameter describing the kinetics of the sensillar potential.…”

Section: Acquisition Protocols and Data Analysismentioning

confidence: 99%

“…Quantity coding in response to adapting pheromone stimuli has been studied in extracellular tip recordings in saturniid moths, in which the different terms 'short-term' versus 'long-term' adaptation were coined for insect olfaction (Zack, 1979;Zack-Strausfeld and Kaissling, 1986;Kaissling et al, 1986Kaissling et al, , 1987. Concerning ORNs of the hawkmoth Manduca sexta, however, there is only one study about olfactory adaptation in temporal coding of pheromone pulse trains (Marion-Poll and Tobin, 1992) and a few studies on quality odour coding (Kaissling et al, 1989;Kalinová et al, 2001).…”

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confidence: 99%

Adaptation in pheromone-sensitive trichoid sensilla of the hawkmoth Manduca sexta

Dolzer

Fischer

Stengl

2003

Journal of Experimental Biology

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While the transduction cascade of pheromone-sensitive olfactory receptor neurons (ORNs) in insects has been thoroughly investigated in vitro (Breer et al., 1988Boekhoff et al., 1990Boekhoff et al., , 1993Stengl et al., 1992;Stengl, 1993Stengl, , 1994Wegener et al., 1993Wegener et al., , 1997, little is known about olfactory adaptation and the mechanisms involved. Adaptation is a universal characteristic of receptor cells of all sensory modalities (Burkhardt, 1961). There are different definitions for the term 'adaptation' but implicit in any definition is a decrease in sensitivity due to the influence of a previous (conditioning) stimulus (Zack, 1979).Moths distinguish pheromone mixtures according to the concentration ratios of the different pheromone components. Thus, differentiation of pheromone concentrations is very crucial for recognition of prospective mates. Turbulences and wind velocities determine the structure of the pheromone filaments that stimulate the antenna of a flying moth. Thus, adapting and non-adapting pheromone stimuli of variable concentrations and stimulus durations reach different parts of the antenna at various time intervals. It is still unknown how the moth can recognize relevant pheromone ratios in various states of adaptation. In our study of pheromone-sensitive ORNs, we examine how adapting pheromone stimuli affect the encoding of different pheromone concentrations (quantity coding) in the intact moth. We distinguish the rapidly (within seconds to minutes) reversible reduction of sensitivity due to prior stimulation (short-term adaptation) from the decline in excitation, as seen during a phasic-tonic response to a stimulus of long duration (desensitization; Zufall and Leinders-Zufall, 2000). Thus, we compare quantity coding in response to short (50·ms) and long (1000·ms) pheromone stimuli, as possibly encountered during flight to the calling female. We do not examine the more slowly occurring (within several minutes to hours) reduction of sensitivity due to In extracellular tip recordings from long trichoid sensilla of male Manduca sexta moths, we studied dose-response relationships in response to bombykal stimuli of two different durations in the adapted and the non-adapted state. Bombykal-responsive cells could be distinguished from non-bombykal-sensitive cells in each trichoid sensillum because the bombykal-responsive cell always generated the action potentials of larger initial amplitude. The bombykal cell, which was recorded at a defined location within a distal flagellar annulus, can resolve at least four log 10-units of pheromone concentrations but is apparently unable to encode all stimulus durations tested. Parameters of the amplitudemodulated sensillar potential and the frequencymodulated action potential responses were examined in different states of adaptation. Evidence is presented for the existence of several mechanisms of adaptation, which affect distinct steps of the transduction cascade. After adapting pheromone stimuli, the sensillar potential rises to a lower ampl...

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“…We thus decided to adjust the pressure of all stimuli to 5 psi to facilitate stimulus access to aesthetascs through their dense packing and to distributed chemosensilla without eliciting strong mechanical responses that would have impeded the correct measurement of chemical responses. Responses of chemoreceptor neurons depend not only on the stimulus characteristics (quality, quantity), but also on previous chemosensory experience via the process of adaptation (Kaissling et al, 1987). When chemoreceptor neurons are adapted by a stimulus, responses to subsequent stimuli are reduced.…”

Section: Determination Of Recording Parametersmentioning

confidence: 99%

New electroantennography method on a marine shrimp in water

Machon

Ravaux

Zbinden

et al. 2016

Journal of Experimental Biology

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Antennular chemoreception in aquatic decapods is well studied via the recording of single chemoreceptor neuron activity in the antennule, but global responses of the antennule (or antennae in insects) by electroantennography (EAG) has so far been mainly restricted to aerial conditions. We present here a well-established underwater EAG method to record the global antennule activity in the marine shrimp Palaemon elegans in natural (aqueous) conditions. EAG responses to food extracts, recorded as net positive deviations of the baseline, are reproducible, dose-dependent and exhibit sensory adaptation. This new EAG method opens a large field of possibilities for studying in vivo antennular chemoreception in aquatic decapods, in a global approach to supplement current, more specific techniques.

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Adaptation Processes in Insect Olfactory Receptors

Cited by 104 publications

References 15 publications

Distinct signaling of Drosophila chemoreceptors in olfactory sensory neurons

Distinct signaling of Drosophila chemoreceptors in olfactory sensory neurons

Adaptation in pheromone-sensitive trichoid sensilla of the hawkmoth Manduca sexta

New electroantennography method on a marine shrimp in water

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