Adaptation of Tobacco Cells to NaCl

Binzel, Marla L.; Hasegawa, Paul M.; Handa, Avtar K.; Bressan, Ray A.

doi:10.1104/pp.79.1.118

Cited by 160 publications

(135 citation statements)

References 40 publications

Supporting

Mentioning

121

Contrasting

Unclassified

Order By: Relevance

“…In plants, salt stress increases [Ca 2ϩ ] cyt that is presumed to facilitate adaptation (46, 47). Furthermore, plants or cultured cells that are exposed to sublethal levels of salt acquire greater stress adaptive capacity (48,49), and tomato seedlings subjected to hyperosmotic pretreatment exhibit enhanced salt tolerance (50). The results of this study provide direct evidence that hyperosmotic stress, generated by salt or a non-ionic osmotic solute, induces a transient [Ca 2ϩ ] cyt increase in yeast cells, and this [Ca 2ϩ ] cyt transient mediates ionic but not osmotic stress adaptation.…”

Section: Transient Increase In Ca Cytmentioning

confidence: 49%

An Osmotically Induced Cytosolic Ca2+ Transient Activates Calcineurin Signaling to Mediate Ion Homeostasis and Salt Tolerance of Saccharomyces cerevisiae

Matsumoto

Ellsmore

Cessna

et al. 2002

Journal of Biological Chemistry

Self Cite

137

119

View full text Add to dashboard Cite

Section: Transient Increase In Ca Cytmentioning

confidence: 49%

An Osmotically Induced Cytosolic Ca2+ Transient Activates Calcineurin Signaling to Mediate Ion Homeostasis and Salt Tolerance of Saccharomyces cerevisiae

Matsumoto

Ellsmore

Cessna

et al. 2002

Journal of Biological Chemistry

Self Cite

137

119

View full text Add to dashboard Cite

“…Cell suspensions of Nicotiana tabacum L. var Wisconsin 38, adapted to different concentrations of NaCl (up to 600 mM), were maintained according to procedures described previously (1). Unadapted 7 ,um in diameter, at an accelerating voltage of 10 kV the limits of resolution are estimated to be 2-3,m [18]), and a relatively even surface across the cell so that the topography at the various points of analysis would be consistent.…”

Section: Methodsmentioning

confidence: 99%

“…We have previously characterized the growth and osmotic adjustment of Nicotiana tabacum L. var Wisconsin 38 cells (1) adapted to high levels of salinity (428 mm NaCI). Na+ and Cl-, the principal components of the extensive osmotic adjustment exhibited by these cells, accumulated to an average whole cell concentration of 500 and 400 mm, respectively (2).…”

mentioning

confidence: 99%

Intracellular Compartmentation of Ions in Salt Adapted Tobacco Cells

Binzel¹,

Hess²,

Bressan³

et al. 1988

Plant Physiol.

Self Cite

245

129

View full text Add to dashboard Cite

Na+ and Cl-are the principal solutes utilized for osmotic adjustment in cells of Nicotiana tabacum L. var Wisconsin 38 (tobacco) adapted to NaCi, accumulating to levels of 472 and 386 millimolar, respectively, in cells adapted to 428 millimolar NaCI. X-ray microanalysis of unetched frozen-hydrated cells adapted to salt indicated that Na+ and Cl-were compartmentalized in the vacuole, at concentrations of 780 and 624 millimolar, respectively, while cytoplasmic concentrations of the ions were maintained at 96 millimolar. The morphometric differences which existed between unadapted and salt adapted cells, (cytoplasmic volume of 22 and 45% of the cell, respectively), facilitated containment of the excited volume of the x-ray signal in the cytoplasm of the adapted cells. Confirmation of ion compartmentation in salt adapted cells was obtained based on kinetic analyses of 22NaI and 36CI-efflux from cells in steady state. These data provide evidence that ion compartmentation is a component of salt adaptation of glycophyte ceUs.A typical response of many plants to saline environments, particularly halophytes, is to accumulate high intracellular concentrations of Na+ and Cl- (9,11,23,26,28). Since the in vitro activities of enzymes isolated from glycophytes or halophytes are inhibited equally by NaCl (9, 11), it has been generally accepted that the accumulated ions are sequestered in the vacuole and 'compatible solutes,' such as sugars, proline, and glycinebetaine, function to balance the osmotic pressure of the cytoplasm (9,11,26). This ability to compartmentalize Na+ and Cl-has been considered to be a mechanism of tolerance of halophytes (26).Although salt tolerance of glycophytes, particularly agronomic species, is usually attributed to the ability to exclude Na+ and Cl-, especially from the shoot (8, 11), intracellular ion compartmentation also may occur in these species. Despite indications of negative correlations between ion accumulation and salt tolerance in glycophytes, such as tomato (22), rice (29), wheat (27), and maize (12), examination of the data reveals that, even in tolerant genotypes, the accumulated levels of Na+ and Clare substantial. In some instances whole cell Na+ and Cl-accumulation was similar for both tolerant and sensitive genotypes (22,27,29). Thus, the ability to compartmentalize Na+ and Clmay be an underlying determinant of the tolerance not only of halophytes but also of many crop species.Verification of ion compartmentation has been restricted by the difficulty in obtaining reliable measurements of subcellular ion concentrations (7,15,23,30). Despite this, cytoplasmic Na + concentration in leaf cells of Suaeda maritima was determined to be 165 mm by efflux analysis when the whole cell concentration was about 600 mM (28). Data obtained by x-ray microanalysis of frozen hydrated leaves of Atriplex spongiosa (23) revealed that cytoplasmic ion concentrations were considerably lower than those in the vacuole.Results from investigations of glycophytes exposed to salinity have been less conc...

show abstract

“…We have previously shown that cells adapted to near sea water levels of salinity (428 mm NaCI) accumulate high concentrations of Na+ and Cl1 and compartmentalize these ions in the vacuole (2,4). The tonoplast H+-ATPase from these cells exhibited a higher specific H+-transport capacity and ATPase activity than the tonoplast ATPase isolated from unadapted cells (26).…”

mentioning

confidence: 96%

NaCl Regulation of Tonoplast ATPase 70-Kilodalton Subunit mRNA in Tobacco Cells

Narasimhan

Binzel²,

Perez-Prat³

et al. 1991

Plant Physiol.

Self Cite

107

View full text Add to dashboard Cite

osmotica (3, 11, 14). Because cytosolic physiological and biochemical processes apparently are inhibited by high ion concentrations, mechanisms that permit compartmentation of these ions in the vacuole and regulate the concentrations of these ions in the cytosol are of substantial importance for adaptation and growth (11,14,15 for the uphill transport of ions and other solutes into the vacuole. The A/H. across the tonoplast is produced by electrogenic H+ pumps, the H+-ATPase (22), and the H+ translocating pyrophosphatase (17, 25). However, the significance of the pyrophosphatase H+ pump to the generation of the ,AH+ across the tonoplast under physiological conditions is not clear (17,25).H+-ATPases that have been categorized on the basis of similar biochemical properties as V-type enzymes act as H+ pumps in the membranes of a wide variety of acidic compartments in eukaryotes (22). The similarities of the protein sequences of these enzymes have recently been confirmed by analysis of cDNA and genomic clones encoding the major ATPase subunits (12,22). The V-type H+-ATPase is a multimeric enzyme of about 400 to 600 kD that consists of up to nine subunits (22,23). The putative catalytic 70-kD subunit forms a hydrophilic complex with a 60-kD subunit that is the principal component of ATPase activity. The 16-kD proteolipid subunits form a hydrophobic complex that functions in H+ conductance. Additional subunits are postulated to form a stalk connecting these two complexes or to be associated with the proton channel (22).It is reasonable to assume that the V-type H+-ATPase is an enzyme that has to be functional at all times during cell growth. Disruption of the genes encoding the 60-or 70-kD subunits of yeast vacuolar H+-ATPase caused conditional lethality. The mutants grew more slowly than the wild-type cells, failed to grow at neutral pH, and lacked the ability to acidify their vacuoles ( 18,28

show abstract

Adaptation of Tobacco Cells to NaCl

Abstract: Cell lines of tobacco (Nicotiana tabacum L. var Wisconsin 38) were obtained which are adapted to grow in media with varying concentrations of NaCI, up to 35 grams per liter (599 millimolar).

Cited by 160 publications

References 40 publications

An Osmotically Induced Cytosolic Ca2+ Transient Activates Calcineurin Signaling to Mediate Ion Homeostasis and Salt Tolerance of Saccharomyces cerevisiae

An Osmotically Induced Cytosolic Ca2+ Transient Activates Calcineurin Signaling to Mediate Ion Homeostasis and Salt Tolerance of Saccharomyces cerevisiae

Intracellular Compartmentation of Ions in Salt Adapted Tobacco Cells

NaCl Regulation of Tonoplast ATPase 70-Kilodalton Subunit mRNA in Tobacco Cells

Contact Info

Product

Resources

About