Adaptation of the yeast Yarrowia lipolytica to ethanol

Antistress systems of the yeast Yarrowia lipolitica (Review)

“…2). Thus, cross resistance took place [12,13]. It should be noted that yeast cells dem onstrated a higher adaptation potential in the case of cross resistance.…”

“…The changes in the intracellular content of cAMP have been investigated as one of the signaling mole cules in response to the influence of oxidants, temper ature, and ethanol [11][12][13]. Figure 6 shows a short time increase in the content of this nucleotide, which is followed by a decrease in its concentration below the initial level under stress conditions, while the content of cAMP in the absence of stressors (the control) was actually unvaried.…”

“…Thus, for example, during adapta tion to the action of oxidants and other stressors, the activities of catalase and superoxide dismutase (SOD) involved in ROS detoxification increased several times in the cells of Y. lipolytica (Table 2) [11][12][13].…”

“…It has also been shown that the activity of the enzymes main taining cellular redox potential in Y. lipolytica cells increases under stress conditions. Glucose 6 phos phate dehydrogenase provides the level of reducing equivalents, while glutathione reductase maintains the pool of reduced glutathione [11][12][13].…”

“…The concentration and exposure time of the stressors were varied [11][12][13]. Mild stressors (treat ment with small doses of oxidants or ethanol and incu bation at 37°C) made it possible to obtain stress resis tant (adapted) cells.…”