Adaptation of the Experimental Background (ATEB) Method in Voltammetric Determination of Thujone in Herbal Matrices

Abstract

In this work, a new baseline correction method, named the Adaptation of The Experimental Background (ATEB), based on the subtraction of the blank sample signal (registered for solution without an analyte, for example a supporting electrolyte), was introduced. However, the method was something more than the simple subtraction of the blank sample signal. An innovation of the algorithm consisted in improving of the blank sample signal through application of the polynomials. Operation of the algorithm was demonstrated in determination of thujone in the synthetic and real samples. The synthetic samples were composed of two different mixtures of the quasi‐absinthe herbal matrices spiked with 0.7–14 mg L−1 of thujone. The measurements were conducted with or without presence of Cu2+, which is an interfering agent originating from production of absinthe like alcoholic beverages. The real sample was Rodnik's absinthe, likewise spiked with thujone. Application of the ATEB method enabled calculation of the calibration lines with very good correlation (not lower than 0.997).

Multivariate approach in voltammetric identification and simultaneous determination of eugenol, carvacrol, and thymol on boron-doped diamond electrode

“…The experimental background was subtracted by approximation using third-degree polynomials *For 7.40 mg/dm 3 of eugenol, 5.55 mg/dm 3 of carvacrol, and 4.10 mg/dm 3 of thymol - Fig Concentration/mg/dm The background subtraction procedure (baseline correction) was chosen in such a way that the value of the RMSECV was as small as possible. Different types of baseline subtraction strategies have been tested, including PLS Toolbox function which calculates weighted baseline by the second-or third-degree polynomial with autoscaling procedure and next an innovative ATEB procedure [65]. However, they brought no satisfactory results: RMSECV for raw data was 0.38-0.97 mg/dm 3 , and after background subtraction 0.58-1.80 mg/dm 3 , depending on the phenolic terpenoid and the assumed LV number.…”

Classification of six herbal bioactive compositions employing LAPV and PLS‐DA

“…The supporting electrolyte was 5‐mL acetate buffer (pH = 6.0). The composition of the supporting electrolyte and the measurement parameters were determined basing on previous experience …”

“…The composition of the supporting electrolyte and the measurement parameters were determined basing on previous experience. 45,56,57 All signals were registered using the LAPV by adding 30 μL of the sample (three samples for each herbal composition) into the supporting electrolyte. Then, the solution was stirred (ca.…”