Adaptation of the baculovirus-producer Spodoptera exigua Se301 cell line to serum-free medium

Beas‐Catena, Alba; Sánchez-Mirón, A.; Camacho, F. Garcı́a; Molina-Grima, E.

doi:10.1016/j.nbt.2009.06.179

Cited by 1 publication

(2 citation statements)

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“…Currently, the existing methods of biological control of CPB populations use entomopathogenic fungi such as Beauveria bassiana, Metarhizium sp. [3] and bacteria Bacillus thuringiensis [4], but not viruses. However, entomopathogenic virus-based bioagents that are species-specific and generally safe for non-target organisms are valuable tools for developing integrated pest management strategies.…”

Section: Introductionmentioning

confidence: 93%

“…However, entomopathogenic virus-based bioagents that are species-specific and generally safe for non-target organisms are valuable tools for developing integrated pest management strategies. As for other insect pests, positive examples include the nucleopolyhedroviruses of the Baculoviridae family, which are currently used to control a number of Lepidoptera species [4].…”

Section: Introductionmentioning

confidence: 99%

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Identification and full-genome assembly of novel Colorado potato beetle iflavirus and solinvi-like virus with SISPA Sequencing

Antonets,

Bodnev,

Rotskaya

et al. 2023

Preprint

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The Colorado potato beetle is one of the most devastating potato pests widespread in the world. However, its viral pathogens remain highly unexplored. Here using SISPA high-throughput sequencing of Colorado potato beetle (CPB) samples derived from prepupal larvae that died from an unknown infection, we have identified two previously unknown viruses and assembled their full-length genomic sequences. The subsequent genetic and phylogenetic analysis of the obtained sequences demonstrated that the isolated viruses, named Leptinotarsa iflavirus 1 and Leptinotarsa solinvi-like virus 1, are the novel representatives of Iflaviridae and Solinviviridae viral families, respectively. To the best of our knowledge, these are the first sequencing-confirmed insect viruses derived directly from CPB samples. And we also propose that Leptinotarsa iflavirus 1 may be associated with lethal disease in CPB.

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Section: Introductionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%