Adaptation of the AFLP technique as a new tool to detect genetic instability and transposition in interspecific hybrids

Vela, Doris; Guerreiro, María Pilar García; Fontdevila, Antonio

doi:10.2144/000113655

Cited by 12 publications

(9 citation statements)

References 17 publications

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“…AFLP markers were obtained using the Vela protocol [33] where the DNA of each individual was digested by a frequent cutter enzyme ( Mse I) and an infrequent one ( Eco RI) and then ligated to oligonucleotide adapters. Fragments, after linking an adapter to both extremities, are amplified with primers having a supplementary base.…”

Section: Methodsmentioning

confidence: 99%

A Genome-Wide Survey of Genetic Instability by Transposition in Drosophila Hybrids

et al. 2014

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Hybridization between species is a genomic instability factor involved in increasing mutation rate and new chromosomal rearrangements. Evidence of a relationship between interspecific hybridization and transposable element mobilization has been reported in different organisms, but most studies are usually performed with particular TEs and do not discuss the real effect of hybridization on the whole genome. We have therefore studied whole genome instability of Drosophila interspecific hybrids, looking for the presence of new AFLP markers in hybrids. A high percentage (27–90%) of the instability markers detected corresponds to TEs belonging to classes I and II. Moreover, three transposable elements (Osvaldo, Helena and Galileo) representative of different families, showed an overall increase of transposition rate in hybrids compared to parental species. This research confirms the hypothesis that hybridization induces genomic instability by transposition bursts and suggests that genomic stress by transposition could contribute to a relaxation of mechanisms controlling TEs in the Drosophila genome.

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Section: Methodsmentioning

confidence: 99%

A Genome-Wide Survey of Genetic Instability by Transposition in Drosophila Hybrids

et al. 2014

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“…Spontaneous interspecies crosses can induce TE activity, which may explain some of the new phenotypes observed (Vela et al 2011;Guerreiro 2014;Debladis et al 2017). TEs may also play a role in the diploidisation that follows polyploidisation events (Vicient and Casacuberta 2017).…”

Section: Use Of Rtes To Investigate Genetic Variability In Plantsmentioning

confidence: 99%

Use of retrotransposon-derived genetic markers to analyse genomic variability in plants

Kalendar

Amenov

Daniyarov

2019

Functional Plant Biol.

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Transposable elements (TEs) are common mobile genetic elements comprising several classes and making up the majority of eukaryotic genomes. The movement and accumulation of TEs has been a major force shaping the genes and genomes of most organisms. Most eukaryotic genomes are dominated by retrotransposons and minimal DNA transposon accumulation. The ‘copy and paste’ lifecycle of replicative transposition produces new genome insertions without excising the original element. Horizontal TE transfer among lineages is rare. TEs represent a reservoir of potential genomic instability and RNA-level toxicity. Many TEs appear static and nonfunctional, but some are capable of replicating and mobilising to new positions, and somatic transposition events have been observed. The overall structure of retrotransposons and the domains responsible for the phases of their replication are highly conserved in all eukaryotes. TEs are important drivers of species diversity and exhibit great variety in their structure, size and transposition mechanisms, making them important putative actors in evolution. Because TEs are abundant in plant genomes, various applications have been developed to exploit polymorphisms in TE insertion patterns, including conventional or anchored PCR, and quantitative or digital PCR with primers for the 5ʹ or 3ʹ junction. Alternatively, the retrotransposon junction can be mapped using high-throughput next-generation sequencing and bioinformatics. With these applications, TE insertions can be rapidly, easily and accurately identified, or new TE insertions can be found. This review provides an overview of the TE-based applications developed for plant species and assesses the contributions of TEs to the analysis of plants’ genetic diversity.

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“…Technical advances to the protocol introduced by Vos et al . (1995) have presented new opportunities for data analysis (Bensch and Åkesson 2005; Meudt and Clarke 2007), among which are adaptations for the study of hybrids (Vela et al . 2011).…”

Section: Hybridization Of Phragmites In North Americamentioning

confidence: 99%

Hybridization of common reed in North America? The answer is blowing in the wind

et al. 2012

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Adaptation of the AFLP technique as a new tool to detect genetic instability and transposition in interspecific hybrids

Cited by 12 publications

References 17 publications

A Genome-Wide Survey of Genetic Instability by Transposition in Drosophila Hybrids

A Genome-Wide Survey of Genetic Instability by Transposition in Drosophila Hybrids

Use of retrotransposon-derived genetic markers to analyse genomic variability in plants

Hybridization of common reed in North America? The answer is blowing in the wind

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