Adaptation of Root Function by Nutrient-Induced Plasticity of Endodermal Differentiation

Barberon, Marie; Vermeer, Joop E. M.; Bellis, Damien De; Wang, Peng; Naseer, Sadaf; Andersen, Tonni Grube; Humbel, Bruno M.; Nawrath, Christiane; Takano, Junpei; Salt, David E.; Geldner, Niko

doi:10.1016/j.cell.2015.12.021

Cited by 431 publications

(560 citation statements)

References 53 publications

(87 reference statements)

Supporting

Mentioning

515

Contrasting

Unclassified

Order By: Relevance

“…Nevertheless it is now clear that nutrient levels can induce [Ca 2+ ] cyt changes and that downstream Ca 2+ sensors regulate appropriate responses. The nutritional status of the root will have a part to play in determining the [Ca 2+ ] cyt signatures, particularly of the endodermis as the extent of suberization is set by nutrition (Barberon et al, 2016) and suberin lamellae determine endodermal [Ca 2+ ] cyt responses (Moore et al, 2002). To date, potassium, nitrate and boron have been studied.…”

Section: Ca2+ Signaling In Nutrient Deprivation Is An Emerging Areamentioning

confidence: 99%

Calcium-Mediated Abiotic Stress Signaling in Roots

et al. 2016

View full text Add to dashboard Cite

Roots are subjected to a range of abiotic stresses as they forage for water and nutrients. Cytosolic free calcium is a common second messenger in the signaling of abiotic stress. In addition, roots take up calcium both as a nutrient and to stimulate exocytosis in growth. For calcium to fulfill its multiple roles must require strict spatio-temporal regulation of its uptake and efflux across the plasma membrane, its buffering in the cytosol and its sequestration or release from internal stores. This prompts the question of how specificity of signaling output can be achieved against the background of calcium’s other uses. Threats to agriculture such as salinity, water availability and hypoxia are signaled through calcium. Nutrient deficiency is also emerging as a stress that is signaled through cytosolic free calcium, with progress in potassium, nitrate and boron deficiency signaling now being made. Heavy metals have the capacity to trigger or modulate root calcium signaling depending on their dose and their capacity to catalyze production of hydroxyl radicals. Mechanical stress and cold stress can both trigger an increase in root cytosolic free calcium, with the possibility of membrane deformation playing a part in initiating the calcium signal. This review addresses progress in identifying the calcium transporting proteins (particularly channels such as annexins and cyclic nucleotide-gated channels) that effect stress-induced calcium increases in roots and explores links to reactive oxygen species, lipid signaling, and the unfolded protein response.

show abstract

Section: Ca2+ Signaling In Nutrient Deprivation Is An Emerging Areamentioning

confidence: 99%

Calcium-Mediated Abiotic Stress Signaling in Roots

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Its physiological role is to allow controlled and selective uptake of nutrients to enter the stele and to retain them in favourable concentrations for the plant [1][2][3][4][5] The Casparian strip is a highly localized, ring-like lignin deposition around each endodermal cell. It seals the apoplastic space between adjacent endodermal cells thus prevents extracellular diffusion, forcing passage of nutrients across cellular membranes.…”

mentioning

confidence: 99%

Transient cell-specific EXO70A1 activity in the CASP domain and Casparian strip localization

et al. 2017

Self Cite

View full text Add to dashboard Cite

In a striking case of evolutionary convergence, polarized cell layers with ring-like diffusion barriers have evolved in both plant and animal lineages independently. In plants, ring-like Casparian strips become localized by the CASPARIAN STRIP MEMBRANE DOMAIN PROTEINS (CASPs). The mechanism of this striking localization, however, has remained enigmatic. Here we present a genetic screen aimed at isolating determinants of CASP localization. One of the mutants, lord of the rings 2 (lotr2)/exo70a1, displays dramatic de-localization of CASPs into randomly localized microdomains. EXO70A1 is a subunit of the exocyst complex, a central component of secretion in eukaryotes. Irradiation of EXO70 subunit genes in plants has suggested specialization of this conserved complex. Intriguingly, lotr2/exo70a1 does neither affect secretion of the CASPs, nor that of other membrane proteins in the endodermis, thus separating exocyst activity in localization from a general defect in secretion. Our results establish EXO70A1 as a central player in Casparian strip formation, generating a transient positional information that will be translated into a precisely localized cell wall modification.

show abstract

“…The two BTSL genes are predominantly expressed in the root epidermis and cortex, whereas BTS is expressed inside the root stele and in the shoot. BTSL2 is also expressed in the stele in the differentiation zone, but this is where the Casparian strip is not yet formed or still permeable (Nasser et al, 2012;Barberon et al, 2016). Moreover, the BTSL2 promoter-GUS expression was analysed under Fe deficient conditions, when suberinization of the Casparian strip is suppressed to maximize metal and nutrient uptake (Barberon et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

BRUTUS-LIKE proteins moderate the transcriptional response to iron deficiency in roots

Rodríguez-Celma

Green

Connorton

et al. 2017

Preprint

View full text Add to dashboard Cite

Iron is an essential micronutrient but in excess is toxic inside cells. Under iron deficiency, the expression of iron uptake genes is increased, but it is not known how the transcriptional response is controlled to avoid uptake of too much iron. The hemerythrin E3 ligases BRUTUS (BTS) and BTS-LIKE (BTSL) have previously been identified as negative regulators of the iron deficiency response. Our phylogenetic analysis indicated that BTSL proteins are present in dicotyledonous plants only and form a separate clade from BTS homologs. BTSL1 and BTSL2in Arabidopsis thaliana are in a network with nearly all iron uptake genes, whereas BTS is in a shoot-specific network. BTSL1 and BTSL2 are expressed predominantly in the root epidermis and cortex, separate from BTS in the root stele, shoot and embryos. Mutant analysis identified BTSL2 as the dominant paralog of the otherwise redundant BTSL genes. The btsl double mutant had increased protein levels of FIT, the FER-like Iron deficiency-induced Transcription factor, and failed to switch off the transcriptional response upon iron resupply, leading to dramatic iron accumulation in roots and shoots. Protein interaction between the C-terminus of BTSL proteins and FIT indicate that FIT is a direct target for degradation. Taken together, our studies show that BTSL1 and BTSL2 control iron uptake in the epidermis and cortex, upstream of BTS in the vasculature and leaves.

show abstract

Adaptation of Root Function by Nutrient-Induced Plasticity of Endodermal Differentiation

Cited by 431 publications

References 53 publications

Calcium-Mediated Abiotic Stress Signaling in Roots

Calcium-Mediated Abiotic Stress Signaling in Roots

Transient cell-specific EXO70A1 activity in the CASP domain and Casparian strip localization

BRUTUS-LIKE proteins moderate the transcriptional response to iron deficiency in roots

Contact Info

Product

Resources

About