Adaptation of Restraint Stress to Body Weight in the Rat

Abstract

Restraint in a metal tube is a reproducible method of inducing gastric ulcers in rats. However, it may be impossible to compare results from animals of different body weight. To make this comparison possible, a theoretical relation was established between the size of the tube and the body weight of the animal. When using this relation a constant ulcer incidence is obtained in animals of varying weight. Alterations of this factor in this relation allows an increase or decrease in the degree of restraint and the ulcer ratio.

Hypergravity Conditioning on Ileal Movements in Rats

“…The most common physiological disorder underlying the digestive problems is reported to be stress (1,21). As a result of stress, feeding disorders, and the consequent increase or decrease in body weight, have been reported in both humans and experimental animals (7,11,15). Stress affects the gastrointestinal tract function as a part of the visceral response (16,22).…”

Effect of Stress and Dietary Fatty Acids on Allograft Survival in the Rat

“…Stress consisted of 3 h of restraint in a metal tube. The tubes used were as described by Martin et al (9). The characteristics of the tubes were: inner diameter, 4.8 cm; basal area, 18.10 cm2; volume, 380 ml; length, 21cm.…”

“…The cylinder was closed at each end by a removable cap. In our case the rats received 'severe stress', according to the criteria of Martin et al (9). To obtain muscle relaxation the rats initially received a light ether anesthesia to be able to put them in the restraint cages.…”

The Effect of Ambient Temperature on Restraint Ulcer in the Rat

“…After a 24-h fasting, the rats were restrained in metal tubes. The diameter of the tube was adapted to the weight of the rat so as to obtain stress of similar intensity for any weight [16].…”