Adaptation of phenol-degrading Pseudomonas putida KB3 to suboptimal growth condition: A focus on degradative rate, membrane properties and expression of xylE and cfaB genes

Nowak, Agnieszka; Żur-Pińska, Joanna; Piński, Artur; Pacek, Gabriela; Mrozik, Agnieszka

doi:10.1016/j.ecoenv.2021.112431

Cited by 18 publications

(7 citation statements)

References 53 publications

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“…In our studies, the fatty acid composition of the total lip- ids from all three studied strains an increasing the C17-cyclopropanoic acid content according to increasing HCB dose were show n. Such changes in some phenol-tolerant C. testosteroni have been observed [13]. Th e conversion of unsaturated fatty acids to cyclopropane-contained is considered to be the main adaptive response of the bacterial cell to the stressful environmental conditions due to stabilizing the cytoplasmic membrane fl uidity [25]. It has been shown that the increase in membrane rigidity in the presence of fatty acids with a cyclopropane ring is due to the higher melting point of the acids compared to unsaturated fatty acids [10].…”

Section: Fatty Acid Composition Of Comamonas Testosteroni Under Hexac...mentioning

confidence: 53%

Fatty Acid Composition of Comamonas testosteroni under Hexachlorobenzene Loading Conditions

Dimova¹,

Iutynska²

2022

Mikrobiol. Z.

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Changes in the lipid composition in bacterial membranes are considered to be the most important adaptation mechanisms to adverse chemical factors. The aim of the study was to compare the hexachlorobenzene effects on the fatty acid composition of total lipids Comamonas testosteroni. Methods. The study was performed with C. testosteroni UCM B-400 and B-401, B-213 strains. Bacteria were grown in the Luria-Bertrani (LB) liquid medium containing 10 and 20 mg/L of hexachlorobenzene (HCB). After cultivation, the biomass was separated by centrifugation and the fatty acid composition of total lipids was determined through analyzing its methyl esters. To assess the cell membrane properties, such parameters as the lipid unsaturation index, the average carbon chain length of fatty acids, and the membrane viscosity index were determined. Results. In the fatty acids spectrum of C. testosteroni B-400 after cultivation in a medium containing 20 mg/L of HCB, the contents of unsaturated hexadecenoic (C16:1) and octadecenoic (C18:1) acids were lower by 10.6 and 5.5%, respectively, and that of saturated hexadecanoic (C16:0) acid was higher by 8.4%, compared to the control. The fatty acid composition of C. testosteroni B-401 was more stable compared to strain B-400. Collection strain C. testosteroni B-213 compared to strains isolated from soil with high HCB load, in the presence of 10 and 20 mg/L of HCB had the highest relative content of saturated hexadecanoic acid (C16:0) up to 38.33—40.7%. Unsaturated octadecenoic acid decreased at the doses 10 and 20 mg/L to 1.5—2% compared to the control. In all strains under the HCB impact, there was an increase in the relative content of C17-cyclopropanoic acid compared to control variants. Conclusions. C. testosteroni UCM B-400, B-401, and B-213 bacteria under cultivation conditions in HCB-containing medium, decreasing the degree of lipid unsaturation and increasing the relative content of C17-cyclopropanoic acid can be considered as the main mechanisms regulating the cytoplasmic membrane fluidity; the displaying of these protective reactions had a strain trait and did not depend on the adaptation in natural isolating places.

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Section: Fatty Acid Composition Of Comamonas Testosteroni Under Hexac...mentioning

confidence: 53%

Fatty Acid Composition of Comamonas testosteroni under Hexachlorobenzene Loading Conditions

Dimova¹,

Iutynska²

2022

Mikrobiol. Z.

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“…Regarding thermal conditions for the optimum growth and metabolic activity of P. putida, numerous comparative studies showed the highest bacterial activity in the temperature range from 25 to 30 • C, while more precise data are dependent on the cultivation conditions and experimental setup. In particular, the maximum nitrate removal activity of P. putida was found at 25 • C [30]; the phenol degradation by P. putida was nine times longer at 10 • C, compared to the corresponding values at 30 • C [31]; the production of arginine deiminase by P. putida was higher at 25 • C, compared with 37 • C [32]; and the optimum growth of P. putida isolated from Antarctic soils was at 16-28 • C [33]. The optimum temperature for the growth of S. epidermidis in culture was reported to be 37 • C [34].…”

Section: Discussionmentioning

confidence: 85%

Characterization of Biofilm Formation and Bacterial Resistance to Benzalkonium Chloride under Contrasting Cultivation Conditions

et al. 2023

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Benzalkonium chloride (BAC) is one of the most commonly used quaternary ammonium compounds in the pharmaceutical, cosmetic, and food industries. The aim of our study was to compare the physiological responses of Escherichia coli MSCL 332, Pseudomonas putida MCCL 650, and Staphylococcus epidermidis MSCL 333 on 50 mg/L BAC in rich and poor medium (100% and 5% tryptone soya broth (TSB)) in the temperature range from 8 °C to 37 °C, under static and shaking conditions. A high-throughput, 96-well microplate method was used to compare a broad range of cultivation conditions. The effect of BAC on growth, biofilm formation activity, and dehydrogenase and fluorescein diacetate hydrolysis activity was evaluated. Addition of BAC to 100% TSB inhibited biofilm formation at 37 °C by 2.4, 1.8, and 1.6 times for E. coli, P. putida, and S. epidermidis, respectively. In turn, BAC stimulated biofilm formation in E. coli in 5% TSB at 37 °C and 100% TSB at 8 °C, i.e., 1.4 and 1.3 times, respectively. Statistical optimization of broth composition with emphasis on biofilm formation and further testing under experimental conditions was performed with P. putida.

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“…Since there is no proportionality between the rates of MDA production ( Figure 4 ) and those of H 2 O 2 ( Figure 3 ) in P. fluorescens CMA-55, and MDA is only produced when an ROS reacts with unsaturated lipids ( Du et al., 2017 ), then probably occurred enzymatic changes in the fatty acid saturation pattern, resulting in changes in the permeability, as a way of responding to increasing glyphosate concentrations. The ability for enzymatic alterations in fatty acid profile has already been observed in the genus Pseudomonas , specifically in strain Pseudomonas putida KB3, which can change cell membrane saturation and permeability through the enzyme cyclopropane fatty acid synthase during phenol exposure ( Nowak et al., 2021 ). Similar facts were observed in Oenococcus oeni , a bacterium with the capacity to adapt and withstand ethanol stresses through cell surface changes and membrane unsaturated/saturated fatty acid ratio ( Bonomo et al., 2017 ).…”

Section: Resultsmentioning

confidence: 99%

Changes in fatty acid composition as a response to glyphosate toxicity in Pseudomonas fluorescens

Oliveira

Marchi

Emiliano

et al. 2022

Heliyon

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Adaptation of phenol-degrading Pseudomonas putida KB3 to suboptimal growth condition: A focus on degradative rate, membrane properties and expression of xylE and cfaB genes

Cited by 18 publications

References 53 publications

Fatty Acid Composition of Comamonas testosteroni under Hexachlorobenzene Loading Conditions

Fatty Acid Composition of Comamonas testosteroni under Hexachlorobenzene Loading Conditions

Characterization of Biofilm Formation and Bacterial Resistance to Benzalkonium Chloride under Contrasting Cultivation Conditions

Changes in fatty acid composition as a response to glyphosate toxicity in Pseudomonas fluorescens

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