Adaptation of Marek’s disease virus to the Vero continuous cell line

Jaikumar, D.; Read, K.M.; Tannock, Gregory A.

doi:10.1016/s0378-1135(00)00346-1

Cited by 8 publications

(5 citation statements)

References 19 publications

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“…CHCC-OU2 cells have also been used for production of cell lines containing vaccine strains of all three MDV serotypes. Recently, adaptation of MDV strains to the continuous Vero cell line after several rounds of passaging has been described (Jaikumar et al ., 2001 ).…”

Section: Full Textmentioning

confidence: 99%

“…It must be noted, however, that adaptation of viruses to the CHCC-OU2 cells took up to 4 weeks, in which changes in the genetic and antigenic composition of the viruses could occur (Abujoub & Coussens, 1995 , 1997 ). Recently, the continuous Vero cell line was tested for its susceptibility to MDV growth, and after 3 weeks of adaptation, very low titres of MDV-1 and HVT were produced (Jaikumar et al ., 2001 ). Using the permanent SOgE cell line, no adaptation of virulent or vaccine MDV strains was necessary, and in passage level 1, growth of the vaccine virus CVI988 was already comparable with that on CEC.…”

Section: Full Textmentioning

confidence: 99%

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Generation of a permanent cell line that supports efficient growth of Marek′s disease virus (MDV) by constitutive expression of MDV glycoprotein E

Schumacher

Tischer

Teifke

et al. 2002

Journal of General Virology

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A recombinant cell line (SOgE) was established, which was derived from the permanent quail muscle cell line QM7 and constitutively expressed the glycoprotein E (gE) gene of Marekhs disease virus serotype 1 (MDV-1). The SOgE cell line supported growth of virulent (RB-1B) and vaccine (CVI988, 584Ap80C) MDV-1 strains at a level comparable with that of primary chicken embryo cells (CEC). The SOgE cell line was used to produce a vaccine against Marekhs disease. Chickens were immunized at 1 day old with 10 3 p.f.u. CVI988 produced on either CEC or SOgE cells. Challenge infection was performed at day 12 with hypervirulent Italian MDV-1 strain EU1. Whereas 7/7 or 6/6 animals, respectively, immunized with SOgE or QM7 cells alone developed Marekhs disease, only 1/8 animals from both CVI988-immunized groups exhibited signs of disease, suggesting that SOgE cells are a valuable permanent cell culture system for MDV-1 vaccine production. Marek's disease virus (MDV), an alphaherpesvirus, occurs worldwide and induces Marekhs disease, which is characterized by T cell lymphomas, polyneuritis, immunosuppression and atherosclerosis (Calnek et al., 1983 ; Calnek, 2001). Three MDV serotypes have been identified, of which only serotype 1 (MDV-1) is pathogenic ; serotype 2 (MDV-2) and serotype 3 (herpesvirus of turkeys, HVT) do not cause disease. MD can be controlled by immunization, but MDV strains have a tendency towards increased virulence despite vaccination, and so-called virulent (v), very virulent (vv) and very virulent plus (vvj) strains have evolved with time (Benton &

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Section: Full Textmentioning

confidence: 99%

Section: Full Textmentioning

confidence: 99%

Generation of a permanent cell line that supports efficient growth of Marek′s disease virus (MDV) by constitutive expression of MDV glycoprotein E

Schumacher

Tischer

Teifke

et al. 2002

Journal of General Virology

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“…The experiments to adapt the HVT FC-126 to the baby hamster kidney cell line (BHK 21 ) were not successful. Further experiments with VERO cell lines (Jaikumar et al, 2001) have shown that HVT FC-126 and Marek's disease virus Serotype 1 can be adapted for growth and multiplication. The adaptation period for HVT FC-126 in VERO cells was between 5 to 10 cycles, compared to the shorter adaptation period of HVT FC-126 in the PLA and GL-4 (2 to 3 cycles).…”

Section: Discussionmentioning

confidence: 99%

“…HVT FC 126 can be adapted to them because of its cultivation in the presence of SR-2.0552P (Serum replacement based on the porcine ocular fluid) instead of FCS (Fetal Calf Serum) (Filipi~et al, 2002;Filipi~et al, 2007). It was also found that MDV, as well as HVT, can be adapted to the VERO (African Green Monkey kidney) cells (Jaikumar et al, 2001). Production of vaccine viruses of MD in avian cell cultures or chicken embryos in the past and in the recent history proved to be the source of vaccine contamination.…”

Section: Introductionmentioning

confidence: 97%

Multiplication of HVT FC-126 (Herpesvirus turkey) virus in the kidney cell lines of no avian origin

Filipič¹,

Gottstein²,

Sladoljev³

et al. 2010

Acta vet. (Beogr.)

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The presented experiments were aimed to cultivate and multiplicate HVT FC-126 in the PLA (Adult pig kidney) and GL-4 (Gerbil kidney) cell lines. Two different HVT FC-126 vaccine strains were used: Marikal SPF (Veterina d.o.o., Croatia) and Lyomarex (Merial, USA). They were adapted to the PLA and GL-4 cell lines. After adaptation, they were titrated on PLA (TCID50 24.23) and GL-4 (TCID50 24.96). On both cell lines they show similar CPE (cytophatic effect). The difference between them was detected using Real Time PCR, which was also positive by agarose gel analysis for the virus contained in Lyomarex, but not in the Marikal SPF. It can be concluded that both cell lines are sensitive to HVT FC-126 and the virus can be multiplied in high titers though much lower than in the calf intestinal epithelial cell line (CIEB) cells (TCID50 27.93)

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“…However, long periods of MDV adaptation to the cell culture were necessary, and changes in genetics or antigenicity could occur during the adaptation period. Similarly, a continuous Vero cell line was reported to produce low titres of MDV virus after long adaptation (Jaikumar et al, 2001). A spontaneously immortalized CEF cell line, DF-1 (Himly et al, 1998;Schaefer-Klein et al, 1998), was shown to support growth of virulent MDV (Niikura et al, 2011).…”

Section: Introductionmentioning

confidence: 96%

Efficient Marek's disease virus (MDV) and herpesvirus of turkey infection of the QM7 cell line that does not contain latent MDV genome

2014

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Marek's disease virus (MDV; also known as Gallid herpesvirus 2, MDV-1) causes oncogenic disease in chickens producing clinical signs that include lymphomas, visceral tumours, nerve lesions, and immunosuppression. MDV vaccines are widely used and mostly produced using primary cells: chicken embryo fibroblast cells, duck embryo fibroblast cells, chicken embryo kidney cells or chicken kidney cells. An immortalized cell line that can be used to manufacture the virus has long been desired. In this report, we demonstrate that QM7 cells were susceptible to infection with either MDV or herpesvirus of turkey (HVT; also known as Meleagrid herpesvirus 1, MDV-3). Polymerase chain reaction analysis with primers amplifying selected MDV genes revealed that QM7 cells did not contain these sequences. However, MDV genes were detected in QT35 cells, which have been reported to harbour latent MDV virus. Transfection of naked MDV DNA initiated efficient infection of QM7 cells. In addition, QM7 cell lysate, clarified supernatant, and QM7 cell pellet infected with MDV were negative for reverse transcriptase activity, indicating absence of endogenous retrovirus. QM7 cells were also found to be free of other avian pathogens in a chick embryo inoculation test. In vivo studies of MDV growing in QM7 cells showed the virus retained its pathogenicity and virulence. In ovo experiments demonstrated that both HVT and MDV propagated in QM7 cells did not interfere with hatchability of injected eggs, and viruses could be re-isolated from hatched chicks. The results suggest that QM7 could be a good host cell line for growing both MDV and HVT.

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Adaptation of Marek’s disease virus to the Vero continuous cell line

Cited by 8 publications

References 19 publications

Generation of a permanent cell line that supports efficient growth of Marek′s disease virus (MDV) by constitutive expression of MDV glycoprotein E

Generation of a permanent cell line that supports efficient growth of Marek′s disease virus (MDV) by constitutive expression of MDV glycoprotein E

Multiplication of HVT FC-126 (Herpesvirus turkey) virus in the kidney cell lines of no avian origin

Efficient Marek's disease virus (MDV) and herpesvirus of turkey infection of the QM7 cell line that does not contain latent MDV genome

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