Adaptation of<i>Saccharomyces cerevisiae</i>to toxic manganese concentration triggers changes in inorganic polyphosphates

Andreeva, N. A.; Ryazanova, L. P.; Дмитриев, В. В.; Kulakovskaya, T. V.; Kulaev, I. S.

doi:10.1111/1567-1364.12049

Cited by 31 publications

(32 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Vacuolar polyP serves a central role in maintaining phosphate homeostasis, sequestering phosphate during growth in phosphate-rich environments (38), and releasing phosphate during the cell cycle to provide the building blocks for DNA replication (40). Moreover, polyP chelates metals, such as manganese and cadmium, thereby preventing metal-induced cellular damage (41,42), and sequesters arginine to increase nitrogen storage without affecting the osmotic pressure (43). One other eukaryotic polyP-generating system has been proposed to exist in Dictyostelium discoideum, which, in addition to a bacterial PPK homologue, contains a tripartite complex of actin-related proteins that was found to synthesize polyP ex vivo (44).…”

Section: Polyp In Eukaryotic Organismsmentioning

confidence: 99%

Inorganic polyphosphate, a multifunctional polyanionic protein scaffold

Xie

Jakob

2019

Journal of Biological Chemistry

135

105

View full text Add to dashboard Cite

Section: Polyp In Eukaryotic Organismsmentioning

confidence: 99%

Inorganic polyphosphate, a multifunctional polyanionic protein scaffold

Xie

Jakob

2019

Journal of Biological Chemistry

135

105

View full text Add to dashboard Cite

“…In the presence of toxicants, when a carbon source is sufficient for the microorganism growth, polyphos phates play the role of a shunt factor, providing energy storage. Such an accumulation of polyphosphates is known in yeasts cultivated in the presence of toxic concentrations of heavy metals [14]. It is possible that a similar shunt role for polyphosphates could exist in bacteria during the degradation of foreign compounds.…”

Section: Resultsmentioning

confidence: 90%

“…Polyphosphates were determined in vivo by staining with 4',6 diamidino 2 phenylindole (DAPI) (Sigma, United States) having a maximum of the fluorescence at 456 nm [14]. A DAPI solution of 5 μL (1.0 mg/mL) was added to 100 μL of cell suspension.…”

Section: Methodsmentioning

confidence: 99%

The role of mineral phosphorus compounds in naphthalene biodegradation by Pseudomonas putida

Пунтус

Ryazanova

Zvonarev

et al. 2015

Appl Biochem Microbiol

View full text Add to dashboard Cite

Inorganic polyphosphates, linear polymers of orthophosphoric acid, are not only reserve phospho rus compounds of microorganisms but are also impor tant regulators involved in the switching of genetic programs and stress adaptation in bacteria [1][2][3]. Bio degradation of exogenous compounds requires signif icant restructuring of the bacterium metabolism, with a large group of genes being expressed. It has been shown that, in degrading polychlorinated biphenyl, the Pseudomonas sp. strain B4 accumulates large elec tron-dense granules mainly consisting of phosphates, according to the data of X ray microanalysis [4]. An increase in the content of polyphosphates was proven by an enzymatic method. It is believed that a parallel increase in the content of the GroEl stress protein and reactive oxygen species is evidence of the involvement of polyphosphates in the adaptation of bacteria to the consumption of polychlorinated biphenyl, which serve not only as a carbon source but also as chemical stress factors [4].P. putida KT2440 mutants deficient in polyphos phate kinase, the main enzyme synthesizing polyphos phates in bacteria, differed not only by the decreased content of polyphosphates (down to 15% of the con trol); they were also more sensitive to ultraviolet, p lac tam antibiotics, and Cd 2+ , and Cu 2+ [5]. P. putida KT2440 contained the pWWO plasmid for toluene bio degradation, which provides for the degradation of m xylene. In cultivation with this substrate, the strain mutant in the polyphosphate kinase gene had a longer lag phase as compared to the parent strain [5].These data suggest that the interrelation between the polyphosphate metabolism and biodegradation of for eign compounds by bacteria provides for cell adaptation to the assimilation of hard to reach and toxic sources of carbon. Thus, it is reasonable to reveal the features of phosphate consumption by bacteria from the media and those of the accumulation of inorganic polyphosphates during the biodegradation of such compounds.The purpose of the study is to investigate the effect of phosphate in the culture medium on the growth of P. putida BS 3701 cells and the characteristics of poly phosphate accumulation during naphthalene degra dation. METHODSThe P. putida BS 3701 strain, which is capable of degradation of polycyclic aromatic hydrocarbons, was used in the study. The strain was obtained from the collection of the Laboratory of Plasmid Biology of the Skryabin Institute of Biochemistry and Physiology of Microorganisms of the Russian Academy of Sciences (SIBPM, RAS).To cultivate the bacteria, Evans (E) mineral medium was used with a pH of 7.0-7.2 and the follow ing composition (mM): K 2 HPO 4 , 50; NH 4 C1, 5; Na 2 SO 4 , 0.1; MgCl 2 , 0.062 (μM); CaC1 2 , 1.0; and (NH 4 ) 6 Mo 7 O 24 ⋅ 4H 2 O, 0.005 [6]. The medium was Abstract-The effect of phosphate concentration in the culture medium on the growth and naphthalene deg radation by Pseudomonas putida BS 3701 was studied. The limiting concentration of phosphate was 0.4 mM and 0.1 mM under cultivation i...

show abstract

“…PolyP appears to regulate apoptosis by inducing activation of caspase-3 in human plasma cells [88]. This polyanion also chelates metals, such as manganese and cadmium, blocking metalinduced cell damage [89,90].…”

Section: Polyp In Eukaryotesmentioning

confidence: 99%

Inorganic Polyphosphates Are Important for Cell Survival and Motility of Human Skin Keratinocytes and Play a Role in Wound Healing

Simbulan-Rosenthal¹,

Carney²,

Gaur³

et al. 2020

Contemporary Topics About Phosphorus in Biology and Materials

View full text Add to dashboard Cite

Inorganic polyphosphate (polyP) is a simple ancient polymer of linear chains of orthophosphate residues linked by high energy phospho-anhydride bonds ubiquitously found in all organisms. Despite its structural simplicity, it plays diverse functional roles. polyP is involved in myriad of processes including serving as microbial phosphagens, buffer against alkalis, Ca 2+ storage, metal-chelating agents, pathogen virulence, cell viability and proliferation, structural component and chemical chaperones, and in the microbial stress response. In mammalian cells, polyP has been implicated in blood coagulation, inflammation, bone differentiation, cell bioenergetics, signal transduction, Ca 2+-signaling, neuronal excitability, as a protein-stabilizing scaffold, and in wound healing, among others. This chapter will discuss (1) polyP metabolism and roles of polyP in prokaryotic and eukaryotic cells, (2) the contribution of polyP to survival, cell proliferation, and motility involved in wound healing in human skin keratinocytes, (3) the use of polyP-containing platelet-rich plasma (PRP) to promote wound healing in acute and chronic wounds, including burns, and (4) the use of polyP-containing PRP in excisional wound models to promote faster healing. While polyP shows promise as a therapeutic agent to accelerate healing for acute and chronic wounds, the molecular mechanisms as a potent modulator of the wound healing process remain to be elucidated.

show abstract

Adaptation ofSaccharomyces cerevisiaeto toxic manganese concentration triggers changes in inorganic polyphosphates

Cited by 31 publications

References 31 publications

Inorganic polyphosphate, a multifunctional polyanionic protein scaffold

Inorganic polyphosphate, a multifunctional polyanionic protein scaffold

The role of mineral phosphorus compounds in naphthalene biodegradation by Pseudomonas putida

Inorganic Polyphosphates Are Important for Cell Survival and Motility of Human Skin Keratinocytes and Play a Role in Wound Healing

Contact Info

Product

Resources

About