Adaptation De<i>Ceratocystis</i>A La Vie Parasitaire chez L'animal — Etude De L'aquisition D'un Pouvoir Pathogene Comparable A Celui<i>De sporo thrix schenckii</i>

Mariat, F.

doi:10.1080/00362177185190421

Cited by 54 publications

(22 citation statements)

References 12 publications

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“…However, it was also found that the absorbed serum reacted only with the clinical isolate of S. schenckii. These data support the hypothesis of Ceratocystis-Sporothrix complex proposed by Mariat (1971) in the point that both species have at least one major common antigen. Andrieu et al (1971) made a comparative study of 3 strains of S. schenckii from human sporotrichosis and Cerato-cystis species from nature by the use of agar-gel precipitation method.…”

Section: Discussionsupporting

confidence: 90%

Antigenic similarity betweenCeratocystisspecies andSporothrix schenckiias observed by immunofluorescence

1976

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Antigenic properties of Sporothrix schenckii and 2 species of Ceratocystis (C. stenoceras and C. ulmi) were compared by indirect immunofluoreseent staining technique. Both species of Ceratocystis and 2 strains of S. schenckii reacted equally well with the unabsorbed antisera against 2 strains ofS. schenckii, indicating that these three species share common antigen. Tile absorption experiments showed that only S. schenekii human strain demonstrated a unique antigen. These results support the hypothesis of Ceratocystis-Sporothrix complex in the point that both species have one major common antigen.Mariat (1971) proposed that a particular Ceratocystis species (ascomycetes), C. stenoceras, might be the perfect form of Sporothrix schenckii, based upon the similarity of the morphological characteristics of both species and the mode of experimental infection into hamster. Very recently, Mariat and co-worker gave additional findings in favour of this hypothesis by the ecological study (Mariat, 1975) and the analysis of lipids compositions (De Bieuvre & Mariat, 1975).To obtain further evidence for the hypothesis of Ceratocystis-Sporothrix complex, antigenic properties of S. schenckii and 2 Ceratocystis species, namely C. stenoceras and C. ulmi were compared serologically by indirect immunofluorescent staining technique in the present study. Antisera against 2 strains of S. schenckii isolated from different sources and the absorbed antiserum which was found to react with some of the clinical isolates (Nishikawa et al., 1975), were employed to clarify the serologic relationship between strains of Ceratocystis species and S. schenckii. MATERIALS AND METHODS MicroorganismsSporothrix schenckii KO 4606 was isolated from a patient with cutaneous lymphatic sporotrichosis and has been maintained in our laboratory. S. schenckii Kurume No. 23 was isolated from the soil in Ohmuta City, Kyushu and was kindly supplied from the Department of Dermatology, Kurume University School of Medicine. C. stenoceras Duke No. 922 and C. ulmi Duke No. 948 were kindly supplied by Professor R. Fukushiro of Kanazawa University. Preparation of antiseraHeat killed yeast cells of S. schenckii prepared from KO 4606 and Kurume No. 23 were used for the preparation of antisera. Immunisation procedure was described in detail in our previous paper (Nishikawa et al., 1975). Yeast cells of the above strains were obtained by culturing the fungi on BHI agar slant containing 1 ~o Dextrose, 0.5 ~ Yeast extract (Difco, Detroit, Mich.) for several days at 35 °C. Absorption of antiseraAntisera to strains Kurume No. 23 and KO 4606 were absorbed reciprocally with each heterologous strain as described elsewhere (Nishikawa et al., 1975). The anti-

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Section: Discussionsupporting

confidence: 90%

Antigenic similarity betweenCeratocystisspecies andSporothrix schenckiias observed by immunofluorescence

1976

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“…This phenomenon, which also occurs in other fungi (Mouchacca andGams 1993, Thorn andBarron 1986), explains the proposal of incorrect synonymies (de Hoog et al 1974, Marimon et al 2007, de Meyer et al 2008) and erroneous anamorph-teleomorph connections in the past, such as those of S. schenckii and O. stenoceras (Taylor 1970, Mariat 1971, de Hoog 1974 or S. inflata and O. bragantinum (Pfenning and Oberwinkler 1993). It also might explain the presence of only subtle morphological differences between the taxa proposed in this study and S. inflata (see RESULTS).…”

Section: Discussionmentioning

confidence: 88%

Sporothrix brunneoviolaceaandSporothrix dimorphospora, two new members of theOphiostoma stenoceras-Sporothrix schenckiicomplex

Madrid¹,

Gené²,

Cano³

et al. 2010

Mycologia

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Sporothrix inflata is a saprobic member of the Ophiostoma stenoceras-Sporothrix schenckii species complex, reported mainly from soil. Ophiostoma bragantinum, an ascomycete described from Brazil, has been proposed as its possible teleomorph. Previous studies revealed that Sporothrix inflata is phenotypically and genetically variable, suggesting the existence of cryptic species. During a continued survey on the biodiversity of microfungi from different countries, seven isolates morphologically similar to S. inflata were obtained from soil samples collected in Spain and USA. In this study their phenotypic features and phylogenetic relationships were assessed. DNA sequence data of two nuclear loci revealed that these isolates correspond to two unnamed clades in S. inflata s.l., one of which also included the type strain of Humicola dimorphospora, a species that traditionally has been considered a synonym of S. inflata. These two groups are proposed herein as Sporothrix brunneoviolacea sp. nov. and Sporothrix dimorphospora comb. nov. S. brunneoviolacea is characterized phenotypically by the production of a diffusible violet-brown pigment in culture and mostly globose, pigmented, lateral blastoconidia. On the other hand S. dimorphospora lacks diffusible pigments and shows mostly subglobose to obovoid pigmented lateral blastoconidia. In contrast to the type strain of S. inflata S. brunneoviolacea and S. dimorphospora assimilate raffinose. The phylogenetic analysis suggested that the proposed anamorph-teleomorph connection between S. inflata and O. bragantinum might not be correct.

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“…Note 4 : Sporothrix schenckii was treated for some years as asexual morph of O. stenoceras (Taylor, 1970, Mariat, 1971, de Hoog, 1974). However, De Beer et al.…”

Section: Taxonomy and Nomenclatormentioning

confidence: 99%

The divorce of Sporothrix and Ophiostoma: solution to a problematic relationship

Beer

Duong

Wingfield

2016

Studies in Mycology

168

134

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One of the causal agents of human sporotrichosis, Sporothrix schenckii, is the type species of the genus Sporothrix. During the course of the last century the asexual morphs of many Ophiostoma spp. have also been treated in Sporothrix. More recently several DNA-based studies have suggested that species of Sporothrix and Ophiostoma converge in what has become known as Ophiostoma s. lat. Were the one fungus one name principles adopted in the Melbourne Code to be applied to Ophiostoma s. lat., Sporothrix would have priority over Ophiostoma, resulting in more than 100 new combinations. The consequence would be name changes for several economically important tree pathogens including O. novo-ulmi. Alternatively, Ophiostoma could be conserved against Sporothrix, but this would necessitate changing the names of the important human pathogens in the group. In this study, we sought to resolve the phylogenetic relationship between Ophiostoma and Sporothrix. DNA sequences were determined for the ribosomal large subunit and internal transcribed spacer regions, as well as the beta-tubulin and calmodulin genes in 65 isolates. The results revealed Sporothrix as a well-supported monophyletic lineage including 51 taxa, distinct from Ophiostoma s. str. To facilitate future studies exploring species level resolution within Sporothrix, we defined six species complexes in the genus. These include the Pathogenic Clade containing the four human pathogens, together with the S. pallida-, S. candida-, S. inflata-, S. gossypina- and S. stenoceras complexes, which include environmental species mostly from soil, hardwoods and Protea infructescences. The description of Sporothrix is emended to include sexual morphs, and 26 new combinations. Two new names are also provided for species previously treated as Ophiostoma.

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Adaptation DeCeratocystisA La Vie Parasitaire chez L'animal — Etude De L'aquisition D'un Pouvoir Pathogene Comparable A CeluiDe sporo thrix schenckii

Cited by 54 publications

References 12 publications

Antigenic similarity betweenCeratocystisspecies andSporothrix schenckiias observed by immunofluorescence

Antigenic similarity betweenCeratocystisspecies andSporothrix schenckiias observed by immunofluorescence

Sporothrix brunneoviolaceaandSporothrix dimorphospora, two new members of theOphiostoma stenoceras-Sporothrix schenckiicomplex

The divorce of Sporothrix and Ophiostoma: solution to a problematic relationship

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