Adaptation and characteristics of replication of a strain of avian reovirus in vero cells

Wilcox, G. E.; Robertson, Marcus; Lines, A.D.

doi:10.1080/03079458508436234

Cited by 20 publications

(9 citation statements)

References 13 publications

(10 reference statements)

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“…The growth curve experiments confirm the findings of Wilcox et al (1985) that reoviruses are more highly cell-associated in Vero cells than in avian cells. However, even after adaptation of the reovirus to Vero by 15 passages, it appeared to have maintained its ability to grow in liver cells, and the high titres of virus detected in growth curves in liver cells illustrated this.…”

Section: Text-fig 3 Growth Curves For Vero-adapted Reovirus After Asupporting

confidence: 78%

“…Wilcox et al (1985) were able to adapt only one of six Australian reoviruses to Vero cells, but Hussain and Spradbrow (1981) found that all three of the Australian strains they examined produced CPE in this cell line. The reasons for these discrepancies are unclear.…”

Section: Text-fig 3 Growth Curves For Vero-adapted Reovirus After Amentioning

confidence: 99%

“…It might be worth comparing the sensitivity of batches of Yero cells from different geographical sources in this way. Wilcox et al (1985) found it necessary to subject viruses to at least four cycles of freezing and thawing before the reovirus could be passaged but here three cycles were found sufficient. In the light of findings by Barta et al (1984) that Vero and CELi cells appeared equally susceptible for a laboratory strain of avian reovirus, an important question to be answered was whether Vero cells could be of value in primary isolation of avian reoviruses.…”

Section: Text-fig 3 Growth Curves For Vero-adapted Reovirus After Amentioning

confidence: 99%

“…However, experiences with one well-established strain of avian reovirus may bear little relationship to those with many different reoviruses, and particularly with regard to primary isolation from field material. Wilcox et al (1985) described recently the adaptation of one of six Australian reovirus strains attempted to replicate in Vero cells. The virus in Vero cells was highly cell-associated, and longer incubation times were required for titration than in primary cultures of CK cells.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of chick embryo liver and vero cell cultures for the isolation and growth of avian reoviruses

1988

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Section: Text-fig 3 Growth Curves For Vero-adapted Reovirus After Asupporting

confidence: 78%

Section: Text-fig 3 Growth Curves For Vero-adapted Reovirus After Amentioning

confidence: 99%

Section: Text-fig 3 Growth Curves For Vero-adapted Reovirus After Amentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparison of chick embryo liver and vero cell cultures for the isolation and growth of avian reoviruses

1988

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“…A similar phenomenon has previously been observed following adaptation and propagation of avian reoviruses in mammalian cell cultures but not in avian cell culture (Yamakami et al, 1984;Wilcox et al, 1985), and was not accompanied by mutational changes in the reovirus genome (Wilcox et al, 1985). Perhaps avian cells in vitro behave differently to those in vivo.…”

Section: Discussionmentioning

confidence: 56%

Rapid passage of avian reovirus in one‐day‐old chicks: Clinical and virological findings

Kibenge

Dhillon

1987

Avian Pathology

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SUMMARYTwo avian reoviruses, strain Reo-25 and isolate W3-492 were inoculated orally in 1-day-old chicks. Three to seven days post inoculation (dpi), the liver, spleen, pancreas, caecal tonsil and duodenum were collected, weighed and titrated in cell culture for their viral content. The different tissue homogenates collected were passaged several times in 1-dayold chicks. Reo-25 virus was passaged only at 3-day intervals and W3-492 virus was passaged at 3-and 7-or 14-day intervals. For both Reo-25 and W3-492 viruses, pathological effects and virus yields in tissues decreased with continued passages.

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Association between the σ protein of avian reovirus and virus-induced fusion of cells

et al. 1999

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Monoclonal antibodies (MAbs) against a 39 kDa (sigma C) protein of the avian reovirus RAM-1 strain inhibited virus-induced fusion of cells and the protein was expressed on the surface of infected cells. The fusion-inhibiting activity of the three MAbs reacting with the sigma C protein suggest two putative epitopes were involved: one epitope recognised by antibody 6H1 and involved in fusion of both Vero and CK cells and a second epitope recognised by antibody 1G1 involved in fusion of Vero cells but not CK cells. The activity of the MAb 6E2 was intermediate, suggesting it may have been located in an intermediate position between the two putative epitopes and inhibited fusion by steric hindrance.

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Adaptation and characteristics of replication of a strain of avian reovirus in vero cells

Cited by 20 publications

References 13 publications

Comparison of chick embryo liver and vero cell cultures for the isolation and growth of avian reoviruses

Comparison of chick embryo liver and vero cell cultures for the isolation and growth of avian reoviruses

Rapid passage of avian reovirus in one‐day‐old chicks: Clinical and virological findings

Association between the σ protein of avian reovirus and virus-induced fusion of cells

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