2016
DOI: 10.1189/jlb.1a0216-090rr
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ADAP plays a pivotal role in CD4+ T cell activation but is only marginally involved in CD8+ T cell activation, differentiation, and immunity to pathogens

Abstract: The adhesion and degranulation promoting adaptor protein (ADAP) is a multifunctional scaffold involved in many different signaling pathways that are important for the function of T cells, including the inside-out and outside-in signaling of integrins, the activation of NF-κB, and the subsequent production of proinflammatory cytokines (e.g., IFN-γ and IL-2). Strikingly, despite its well-established role in T cells, previous studies did not distinguish between CD4 and CD8 T cells, and thus, it is unknown whether… Show more

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Cited by 15 publications
(19 citation statements)
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“…Cells were then allowed to adhere for 30 min at 37°C, unbound cells were carefully washed off with Hanks buffered saline (HBSS, Biochrom AG). Bound cells were counted and calculated as percentage of input (2 × 10 5 Jurkat T cells or 1 × 10 6 mouse T cells) in duplicates or triplicates (47, 48). Conjugate formation was performed as previously described (24, 49).…”
Section: Methodsmentioning
confidence: 99%
“…Cells were then allowed to adhere for 30 min at 37°C, unbound cells were carefully washed off with Hanks buffered saline (HBSS, Biochrom AG). Bound cells were counted and calculated as percentage of input (2 × 10 5 Jurkat T cells or 1 × 10 6 mouse T cells) in duplicates or triplicates (47, 48). Conjugate formation was performed as previously described (24, 49).…”
Section: Methodsmentioning
confidence: 99%
“…The Listeria monocytogenes (Lm) strain 10403S expressing ovalbumin was used for mouse infection experiments as described before (6,41). Frozen stocks of Lm were thawed and subsequently diluted in brain heart infusion medium (BHI) followed by overnight growth on agar plates.…”
Section: Listeria Monocytogenes Infectionmentioning
confidence: 99%
“…In vivo infection of mice with Lm is known to induce effective NK cell activation (33,34) in a process involving a complex cellular network of macrophages, neutrophils, and dendritic cells as well as a plethora of soluble mediators derived from these cells (35,36). Thus, Lm infection, that is well-established in our laboratory (6), represents a suitable tool to study the role of ADAP in NK cell priming, cytokine production and cytotoxicity during in vivo infection.…”
Section: Introductionmentioning
confidence: 96%
See 1 more Smart Citation
“…The role of ADAP specifically in CD8+ T cell function in pathogen clearance has been tested in mice with inconclusive results. In one study, by Li et al, loss of ADAP in mice led to increased Influenza virus A-induced mortality, whereas in another study, the ability of ADAP À/À CD8+ T cells to clear similar infection was unimpaired (Parzmair et al 2017). Finally, the ADAP/SKAP1 module can reduce cytotoxicity of CD8+ T cells through positive regulation of the inhibitory molecule, PD-1.…”
Section: Adap Relevance To Human Patients and In Disease Modelsmentioning
confidence: 99%