ADAM17 participates in the protective effect of paeoniflorin on mouse brain microvascular endothelial cells

Abstract

Paeoniflorin (PF), the most abundant active ingredient of traditional Chinese herbal medicine Paeoniae Radix, has been recognized as a potential neuroprotectant due to its remarkable efficacy on mitigating cerebral infarction and preventing the neurodegenerative diseases. However, the precise mechanisms of PF remain incompletely understood. In this study, we first provided evidence for the protective effect of PF on hydrogen peroxide-induced injury on mouse brain microvascular endothelial bEnd.3 cells, and for transactivation of the epidermal growth factor receptor (EGFR) signal induced by PF, suggesting that EGFR transactivation might be involved in the beneficial role of PF. Next, by detecting the phosphorylation of a disintegrin and metalloprotease 17 (ADAM17) at Thr 735 and performing loss-of-function experiments with the ADAM17 inhibitor and ADAM 17-siRNA, we showed that PF-induced transactivation of EGFR and downstream ERKs and AKT signaling pathways were dependent on ADAM17. Furthermore, PF-induced phosphorylation of ADAM17 and the EGFR transactivation were inhibited by the inhibitors of adenosine A1 receptor (A1R) or Src kinase that were applied to cells prior to PF treatment, implying the involvement of A1R, and Src in the activation of ADAM17. Finally, PF reduced the cell surface level of TNF-receptor 1 (TNFR1) and increased the content of soluble TNFR1 (sTNFR1) in the culture media, indicating that PF might enhance the shedding of sTNFR1. Taken together, we conclude that A1R and Src-dependent activation of ADAM17 participates in PF-induced EGFR transactivation and TNFR1 shedding on mouse brain microvascular endothelial cells, which may contributes to the neuroprotective effects of PF.

Astragaloside III activates TACE/ADAM17‐dependent anti‐inflammatory and growth factor signaling in endothelial cells in a p38‐dependent fashion

“…First, we determined whether AS‐III causes phosphorylation of ERK1/2 and AKT kinase, which can be induced by the transactivation of EGFR (Wang et al, ). The cells were treated with 1,000‐nM AS‐III for the indicated times (5, 15, 30, or 60 min), and then the phosphorylation of ERK1/2 and AKT was detected by Western blot analysis.…”

Integrated network-based multiple computational analyses for identification of co-expressed candidate genes associated with neurological manifestations of COVID-19

“…After viral endocytosis is over, ADAM17 directs the shedding of the ectodomain of the receptors 31 and enhances the formation of TNF-α leading to escalation of the cytokine storm 1 . Dysfunction of ADAMs can also exacerbate Alzheimer’s disease condition through the misfolded Aβ pathology 32 , ischaemic stroke 33 and vascular thrombosis 34 via ACE2 and TNF-α receptors. Recently, ADAM10 and ADAM17 have been marked as the risk factors for cerebral infarction and hippocampal sclerosis related epilepsy 35 , respectively.…”

Candidate genes associated with neurological manifestations of COVID-19: Meta-analysis using multiple computational approaches

“…After viral endocytosis is over, ADAM17 directs the shedding of the ectodomain of the receptors 91 , and enhances the formation of TNF-α with escalation of the cytokine storm 3 . Dysfunction of ADAMs can also exacerbate Alzheimer's disease condition through the misfolded Aβ pathology 92 , ischemic stroke 93 and vascular thrombosis 94 via ACE2 and TNF-α receptors. Recently, ADAM10 and ADAM17 have been marked as the risk factors for cerebral infarction and hippocampal sclerosis related epilepsy 95 , respectively.…”