Acylated ghrelin prevents doxorubicin-induced cardiac intrinsic cell death and fibrosis in rats by restoring IL-6/JAK2/STAT3 signaling pathway and inhibition of STAT1

Acylated ghrelin protects against doxorubicin‐induced nephropathy by activating silent information regulator 1

“…The dose of AG and [D‐Lys3]‐GHRP‐6 has been previously shown in our laboratory to raise the levels of AG to the supraphysiological level and to block the peripheral effect of AG, respectively 43 . Similar doses of AG and [D‐Lys3]‐GHRP‐6 were also used by others 49,50 .…”

“…Despite this extensive research on the health benefits of AG ghrelin, its protective effect against DOX‐induced nephropathy is not established yet. Recently, we have demonstrated a potent cardioprotective and anti‐fibrotic effect of AG in DOX‐treated rats, an effect that is mediated by modulating STAT1/STAT3 expression 43 . In another study, we have also shown that this cardioprotective effect of AG involves also the activation of SIRT1 ( in press ).…”

“…No. A05118, respectively, SPI Bio) and following our previously reported method 43 . All measurements were done within 2 days after plasma collection.…”

“…This was done as described in more detail in our previous studies 43,53 . Protein samples of the total cell homogenates or the pre‐precipitated proteins from the nuclear extract (40 µg/well) were separated by sodium dodecyl sulphate (SDS)‐polyacrylamide gel electrophoresis (different ratios, 8%‐15%) and then transferred onto nitrocellulose paper.…”

“…Recently, we have demonstrated a potent cardioprotective and anti-fibrotic effect of AG in DOX-treated rats, an effect that is mediated by modulating STAT1/STAT3 expression. 43 In another study, we have also shown that this cardioprotective effect of AG involves also the activation of SIRT1 (in press). Of note, the stimulatory effect of AG on SIRT1 is well-documented in extra-renal tissues.…”