Acylase enzymes disrupting quorum sensing alter the transcriptome and phenotype of Pseudomonas aeruginosa, and the composition of bacterial biofilms from wastewater treatment plants

Celis, Miguel de; Serrano-Aguirre, Lara; Belda, Ignacio; Liébana-García, Rebeca; Arroyo, Miguel; Marquina, Domingo; Mata, Isabel de la; Santos, Andrés de la Oliva

doi:10.1016/j.scitotenv.2021.149401

Cited by 23 publications

(7 citation statements)

References 70 publications

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“…The application of M. testaceum (AHL-degrading bacterium) protects against the plant pathogen P. carotovorum [ 159 ]. Quorum-quenching enzymes, such as acylases [ 160 ] and lactonases [ 161 ], have shown protective efficacy as anti-QS agents. A significant limitation of using these enzymes in animals is the allergic reactions and immune response against the proteins; however, in plants, these can provide high efficacy and applicability.…”

Section: Applications Of Ahl-dependent Quorum Sensingmentioning

confidence: 99%

Molecular Mechanisms and Applications of N-Acyl Homoserine Lactone-Mediated Quorum Sensing in Bacteria

et al. 2022

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Microbial biodiversity includes biotic and abiotic components that support all life forms by adapting to environmental conditions. Climate change, pollution, human activity, and natural calamities affect microbial biodiversity. Microbes have diverse growth conditions, physiology, and metabolism. Bacteria use signaling systems such as quorum sensing (QS) to regulate cellular interactions via small chemical signaling molecules which also help with adaptation under undesirable survival conditions. Proteobacteria use acyl-homoserine lactone (AHL) molecules as autoinducers to sense population density and modulate gene expression. The LuxI-type enzymes synthesize AHL molecules, while the LuxR-type proteins (AHL transcriptional regulators) bind to AHLs to regulate QS-dependent gene expression. Diverse AHLs have been identified, and the diversity extends to AHL synthases and AHL receptors. This review comprehensively explains the molecular diversity of AHL signaling components of Pseudomonas aeruginosa, Chromobacterium violaceum, Agrobacterium tumefaciens, and Escherichia coli. The regulatory mechanism of AHL signaling is also highlighted in this review, which adds to the current understanding of AHL signaling in Gram-negative bacteria. We summarize molecular diversity among well-studied QS systems and recent advances in the role of QS proteins in bacterial cellular signaling pathways. This review describes AHL-dependent QS details in bacteria that can be employed to understand their features, improve environmental adaptation, and develop broad biomolecule-based biotechnological applications.

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Section: Applications Of Ahl-dependent Quorum Sensingmentioning

confidence: 99%

Molecular Mechanisms and Applications of N-Acyl Homoserine Lactone-Mediated Quorum Sensing in Bacteria

et al. 2022

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“…Tzanov et al found that QQE acyltransferase could reduce the AHL signal ( Figure 5 a) [ 111 ]. The aceleacin A acylase ( Au AAC) and N-acyl homoserine lactone acyltransferase ( Au AHLA) have the same effect [ 112 ]. These enzymes have a QS targeting function.…”

Section: Strategies Of Targeting Initial Adhesion Stagementioning

confidence: 99%

Targeted Anti-Biofilm Therapy: Dissecting Targets in the Biofilm Life Cycle

Liu

Xie

et al. 2022

Pharmaceuticals

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Biofilm is a crucial virulence factor for microorganisms that causes chronic infection. After biofilm formation, the bacteria present improve drug tolerance and multifactorial defense mechanisms, which impose significant challenges for the use of antimicrobials. This indicates the urgent need for new targeted technologies and emerging therapeutic strategies. In this review, we focus on the current biofilm-targeting strategies and those under development, including targeting persistent cells, quorum quenching, and phage therapy. We emphasize biofilm-targeting technologies that are supported by blocking the biofilm life cycle, providing a theoretical basis for design of targeting technology that disrupts the biofilm and promotes practical application of antibacterial materials.

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“…These QQ enzymes, including lactonases, acylases and paraoxonase, catalyse the degradation of QSSMs by either opening their lactone ring or hydrolysing the amide linkage to yield fatty acids and homoserine lactone (Kalia et al ., 2019). QQ acylases have been reported to degrade 3‐oxo‐C12‐HSL, preventing its accumulation (Sio et al ., 2006), altering the biofilm structure and transcriptome of PAO1 (de Celis et al ., 2021), and attenuating its virulence in C. elegans and a mouse model of pulmonary infection (Utari et al ., 2018). Microbial lactonases have exhibited promising QQ potential by attenuating QS and virulence in clinical strains of P. aeruginosa isolated from burn wounds (López‐Jácome et al ., 2019) and also reduced mortality in a murine model of pneumonia by ~fourfold, without altering the bacterial lung count (Hraiech et al ., 2014).…”

Section: Other Inhibitors Of P Aeruginosa Qs Systemsmentioning

confidence: 99%

Repurposing phytochemicals as anti‐virulent agents to attenuate quorum sensing‐regulated virulence factors and biofilm formation in Pseudomonas aeruginosa

Chadha

Harjai

Chhibber

2021

Microbial Biotechnology

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Summary Unregulated consumption and overexploitation of antibiotics have paved the way for emergence of antibiotic‐resistant strains and ‘superbugs’. Pseudomonas aeruginosa is among the opportunistic nosocomial pathogens causing devastating infections in clinical set‐ups globally. Its artillery equipped with diversified virulence elements, extensive antibiotic resistance and biofilms has made it a ‘hard‐to‐treat’ pathogen. The pathogenicity of P. aeruginosa is modulated by an intricate cell density‐dependent mechanism called quorum sensing (QS). The virulence artillery of P. aeruginosa is firmly controlled by QS genes, and their expression drives the aggressiveness of the infection. Attempts to identify and develop novel antimicrobials have seen a sharp rise in the past decade. Among different proposed mechanisms, a novel anti‐virulence approach to target pseudomonal infections by virtue of anti‐QS and anti‐biofilm drugs appears to occupy the centre stage. In this respect, bioactive phytochemicals have gained prominence among the scientific community owing to their significant quorum quenching (QQ) properties. Recent studies have shed light on the QQ activities of various phytochemicals and other drugs in perturbing the QS‐dependent virulence in P. aeruginosa. This review highlights the recent evidences that reinforce the application of plant bioactives for combating pseudomonal infections, their advantages and shortcomings in anti‐virulence therapy.

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Acylase enzymes disrupting quorum sensing alter the transcriptome and phenotype of Pseudomonas aeruginosa, and the composition of bacterial biofilms from wastewater treatment plants

Cited by 23 publications

References 70 publications

Molecular Mechanisms and Applications of N-Acyl Homoserine Lactone-Mediated Quorum Sensing in Bacteria

Molecular Mechanisms and Applications of N-Acyl Homoserine Lactone-Mediated Quorum Sensing in Bacteria

Targeted Anti-Biofilm Therapy: Dissecting Targets in the Biofilm Life Cycle

Repurposing phytochemicals as anti‐virulent agents to attenuate quorum sensing‐regulated virulence factors and biofilm formation in Pseudomonas aeruginosa

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