Acyl-Homoserine Lactones Produced by<i>Pantoea</i>sp. Isolated from the “Maize White Spot” Foliar Disease

Pomini, Armando M.; Paccola-Meirelles, Luzia Doretto; Marsaioli, Anita J.

doi:10.1021/jf063136a

Cited by 16 publications

(25 citation statements)

References 17 publications

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“…Some research groups have attempted to characterize the presence of AHLs in plant tissues. Some time ago it was noted that bioreporters based on Agrobacterium tumefaciens NTL4(pZLR4) mutant cells were able to detect AHL or compounds with counterpart activities in extracts from corn leaves infected by Pantoea agglomerans , the causative agent for maize white spot disease . The same phenomenon has been previously observed in pea seedlings …”

Section: Phytochemical Study Of Sugar Cane and Ahl Identificationsupporting

confidence: 55%

Acyl-homoserine Lactone from Saccharum × officinarum with Stereochemistry-Dependent Growth Regulatory Activity

Olher

Ferreira²,

Souza³

et al. 2016

J. Nat. Prod.

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Acyl-homoserine lactones (AHLs) are a class of compounds produced by Gram-negative bacteria that are used in a process of chemical communication called quorum sensing. Much is known about how bacteria use these chemical compounds to control the expression of important factors; however, there have been few reports about the presence and effects of AHLs in plants. In this study, the phytochemical study of leaves and culms of sugar cane (Saccharum × officinarum) led to the identification of N-(3-oxo-octanoyl)homoserine lactone. Since the absolute configuration of the natural product could not be determined, both R and S enantiomers of N-(3-oxo-octanoyl)homoserine lactone were synthesized and tested in sugar cane culms. The enantiomers caused changes in the mass and length of buds and roots when used at micromolar concentrations. Using the sugar cane RB96-6928 variety, the S enantiomer increased sprouting of roots more effectively than the R enantiomer. Furthermore, scanning electron microscopy showed that both the R and S enantiomers led to more stretched root cells compared with the control.

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Section: Phytochemical Study Of Sugar Cane and Ahl Identificationsupporting

confidence: 55%

Acyl-homoserine Lactone from Saccharum × officinarum with Stereochemistry-Dependent Growth Regulatory Activity

Olher

Ferreira²,

Souza³

et al. 2016

J. Nat. Prod.

Self Cite

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“…L) configured, although the absolute configuration has only been experimentally established in a few cases: by circular dichroism for (S)-3-oxo-C 6 -HSL ((S)-3) from Pectobacterium carotovorum, 7 by chiral GC for (S)-C 6 -HSL from Erwinia psidii as well as (S)-C 6 -HSL and (S)-C 7 -HSL from Pantoea ananatis, [59][60][61] and, using both methods, to elucidate the S configuration of C 4 -HSL (1) produced by another strain of Pantoea sp. 62 The mechanism of AHL biosynthesis is particularly interesting, because the two substrates used adopt roles that drastically deviate from their usual cellular functions, in which SAM normally acts as a biological methyl donor and the acyl-ACPs are prone to yield fatty acids.…”

Section: Introductionmentioning

confidence: 99%

Quorum sensing and bacterial biofilms

2010

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This review describes the chemistry of the bacterial biofilms including the chemistry of their constituents and signalling compounds that mediate or inhibit the formation of biofilms. Systems are described with special emphasis, in which quorum sensing molecules (autoinducers) trigger the formation of biofilms. In the first instance, N-acyl-L-homoserine lactones (AHLs) are the focus of this review, whereas the inter-species signal known as furanosyl borate diester and peptide autoinducers used by Gram-positive bacteria are not discussed in detail. Since the first discovery of an AHL autoinducer from Vibrio fischeri a large and further increasing number of different AHL structures from Gram-negative bacteria have been identified. This review gives a summary of all known AHL autoinducers and producing bacterial species. A few systems are discussed, where biofilm formation is suppressed by enzymatic degradation of AHL molecules or interference of secondary metabolites from other species with the quorum sensing systems of communicating bacteria. Finally, the multi-channel quorum sensing system, the intracellular downstream processing of the signal, and the resulting response of whole populations including biofilm formation are discussed for the Vibrio genus that has been extensively investigated.

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“…This bacterium can survive epiphytically on maize leaves and trigger the disease in its host through a mechanism that remains unclear. According to Pomini, Paccola-Meirelles, and Marsaioli (2007), the quorum-sensing system is used to assess the population size by secreting substances such as acyl homoserine lactones (RUMJANEK;FONSECA;XAVIER, 2004). This mechanism may be associated with P. ananatis pathogenicity (MOROHOSHI et al, 2007), but the role of these signaling molecules in the pathogen is unknown.…”

Section: Introductionmentioning

confidence: 99%

Ice nucleation activity in Pantoea ananatis obtained from maize white spot lesions

et al. 2014

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Maize white spot lesions caused by Pantoea ananatis has contributed substantially to yield reduction of maize crops in many countries, including Brazil. The initial symptoms of the disease include watersoaked lesions on the leaves, which later become necrotic and straw-colored. Basic knowledge regarding the biology and the infection mechanisms of this pathogen is lacking. In this study, 15 P. ananatis isolates obtained from maize white spot lesions were examined for their ice nucleation activity (INA). The INAs of individual bacterial isolates was determined by tube nucleation tests. Bacterial isolates were grown on tryptic soy broth medium and an aliquot of 0.1 mL of culture was added to test tubes containing 1 mL of sterile distilled water. The tubes were packed in an ice bath, which had a temperature below -10°C, for approximately 2 min. Instantaneous formation of ice in the tube revealed a positive INA phenotype of the isolate. Only 9 of the 15 studied isolates showed the INA + phenotype. Pathogenicity tests were performed using whole plants and detached leaves. Symptoms were reproduced in both tests, but only for the inoculations using INA + isolates. Electron microscopy allowed visualization of protein vesicles under outer cell wall of isolates characterized as INA + . Key words: INA, vesicle protein, transmission electron microscopy ResumoLesões de mancha branca do milho causadas por Pantoea ananatis contribuiram substancialmente para a redução da produtividade nos cultivos de milho, em muitos países, incluindo o Brasil. Os sintomas iniciais da doença incluem lesões anasarcas nas folhas, que mais tarde tornam-se necróticas e de cor palha. O conhecimento básico sobre a biologia e os mecanismos de infecção deste patógeno são escassos. Neste estudo, 15 isolados de P. ananatis obtidos a partir de lesões da mancha branca do milho foram examinados quanto à sua atividade de nucleação de gelo (INA). A INA de isolados bacterianos individuais foi determinada por testes de nucleação em tubos. Isolados bacterianos foram cultivados em meio de caldo de soja tríptico e uma alíquota de 0,1 mL de cultura foi adicionada à tubos de ensaio contendo 1 mL de água destilada esterelizada. Os tubos foram colados em banho de gelo, o qual continha temperatura inferior a -10 ºC, por aproximadamente 2 min. A formação de gelo instantânea no tubo revelou um fenótipo INA positivo do isolado. Apenas 9 dos 15 isolados estudados apresentaram o fenótipo INA+. Testes de patogenicidade foram realizados com plantas inteiras e folhas destacadas.

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Acyl-Homoserine Lactones Produced byPantoeasp. Isolated from the “Maize White Spot” Foliar Disease

Cited by 16 publications

References 17 publications

Acyl-homoserine Lactone from Saccharum × officinarum with Stereochemistry-Dependent Growth Regulatory Activity

Acyl-homoserine Lactone from Saccharum × officinarum with Stereochemistry-Dependent Growth Regulatory Activity

Quorum sensing and bacterial biofilms

Ice nucleation activity in Pantoea ananatis obtained from maize white spot lesions

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