Acyl‐Homoserine Lactone‐Based Quorum Sensing in Members of the Marine Bacterial Roseobacter Clade: Complex Cell‐to‐Cell Communication Controls Multiple Physiologies

Buchan, Alison; Mitchell, April; Cude, W. Nathan; Campagna, Shawn R.

doi:10.1002/9781119004813.ch19

Cited by 12 publications

(21 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…5, half of the roseobacters genomes contained more than one pair of luxRI (18/31) and more than two AHL molecules were identified in nine strains. It has been shown that >80% of bacteria in the Roseobacter group possess at least one luxRI gene cassette (Buchan et al ., 2016). Phaeobacter species consistently appear to produce more than three AHLs using 2–3 luxI homologues (Fig.…”

Section: Resultsmentioning

confidence: 99%

Quorum sensing regulates ‘swim‐or‐stick’ lifestyle in the phycosphere

Cong

Ochsenkühn

Shibl

et al. 2020

Environmental Microbiology

View full text Add to dashboard Cite

Summary Interactions between phytoplankton and bacteria play major roles in global biogeochemical cycles and oceanic nutrient fluxes. These interactions occur in the microenvironment surrounding phytoplankton cells, known as the phycosphere. Bacteria in the phycosphere use either chemotaxis or attachment to benefit from algal excretions. Both processes are regulated by quorum sensing (QS), a cell–cell signalling mechanism that uses small infochemicals to coordinate bacterial gene expression. However, the role of QS in regulating bacterial attachment in the phycosphere is not clear. Here, we isolated a Sulfitobacter pseudonitzschiae F5 and a Phaeobacter sp. F10 belonging to the marine Roseobacter group and an Alteromonas macleodii F12 belonging to Alteromonadaceae, from the microbial community of the ubiquitous diatom Asterionellopsis glacialis. We show that only the Roseobacter group isolates (diatom symbionts) can attach to diatom transparent exopolymeric particles. Despite all three bacteria possessing genes involved in motility, chemotaxis, and attachment, only S. pseudonitzschiae F5 and Phaeobacter sp. F10 possessed complete QS systems and could synthesize QS signals. Using UHPLC–MS/MS, we identified three QS molecules produced by both bacteria of which only 3‐oxo‐C16:1‐HSL strongly inhibited bacterial motility and stimulated attachment in the phycosphere. These findings suggest that QS signals enable colonization of the phycosphere by algal symbionts.

show abstract

Section: Resultsmentioning

confidence: 99%

Quorum sensing regulates ‘swim‐or‐stick’ lifestyle in the phycosphere

Cong

Ochsenkühn

Shibl

et al. 2020

Environmental Microbiology

View full text Add to dashboard Cite

show abstract

“…When the population cell density increases, the extracellular concentration of produced autoinducers increases as well, with these molecules then being sensed by the neighbouring cells triggering gene regulation. This type of regulation has been reported in various bacterial groups, and affects the production of different type of bioactive SMs such as polyketides, β-lactams, and phenazines [ 13 , 120 , 121 ]. In theory the effectiveness of the antibiotic is maximized if the entire bacterial population concomitantly produces and releases the molecule(s), thereby increasing its concentration in the immediate environment.…”

Section: Co-cultivation and Other Environmental Cuesmentioning

confidence: 99%

Extending the “One Strain Many Compounds” (OSMAC) Principle to Marine Microorganisms

et al. 2018

View full text Add to dashboard Cite

Genomic data often highlights an inconsistency between the number of gene clusters identified using bioinformatic approaches as potentially producing secondary metabolites and the actual number of chemically characterized secondary metabolites produced by any given microorganism. Such gene clusters are generally considered as “silent”, meaning that they are not expressed under laboratory conditions. Triggering expression of these “silent” clusters could result in unlocking the chemical diversity they control, allowing the discovery of novel molecules of both medical and biotechnological interest. Therefore, both genetic and cultivation-based techniques have been developed aimed at stimulating expression of these “silent” genes. The principles behind the cultivation based approaches have been conceptualized in the “one strain many compounds” (OSMAC) framework, which underlines how a single strain can produce different molecules when grown under different environmental conditions. Parameters such as, nutrient content, temperature, and rate of aeration can be easily changed, altering the global physiology of a microbial strain and in turn significantly affecting its secondary metabolism. As a direct extension of such approaches, co-cultivation strategies and the addition of chemical elicitors have also been used as cues to activate “silent” clusters. In this review, we aim to provide a focused and comprehensive overview of these strategies as they pertain to marine microbes. Moreover, we underline how changes in some parameters which have provided important results in terrestrial microbes, but which have rarely been considered in marine microorganisms, may represent additional strategies to awaken “silent” gene clusters in marine microbes. Unfortunately, the empirical nature of the OSMAC approach forces scientists to perform extensive laboratory experiments. Nevertheless, we believe that some computation and experimental based techniques which are used in other disciplines, and which we discuss; could be effectively employed to help streamline the OSMAC based approaches. We believe that natural products discovery in marine microorganisms would be greatly aided through the integration of basic microbiological approaches, computational methods, and technological innovations, thereby helping unearth much of the as yet untapped potential of these microorganisms.

show abstract

“…In Figure 5, half of the roseobacters genomes contained more than one pair of luxRI (18/31) and more than two AHL molecules were identified in nine strains. It has been shown that >80% of bacteria in the Roseobacter group possess at least one luxRI gene cassette (Buchan et al, 2016). Phaeobacter species consistently appear to produce more than three AHLs using 2−3 luxI homologs ( Fig.…”

Section: Qs Gene Homology and Organization In The Roseobacter Groupmentioning

confidence: 97%

Quorum Sensing Regulates ‘swim-or-stick’ Lifestyle in the Phycosphere

Cong

Ochsenkühn

Shibl

et al. 2020

Preprint

View full text Add to dashboard Cite

Originality-significance statementMotility and biofilm formation are processes regulated by quorum sensing (QS) in bacteria. Both functions are believed to play an important role in interactions between bacteria and phytoplankton. Here, we show that two bacterial symbionts from the microbial community associated with a ubiquitous diatom switch their motile lifestyle to attached cells while an opportunist bacterium from the same community is incapable of attachment, despite possessing the genetic machinery to do so. Further work indicated that the opportunist lacks QS signal synthases while the symbionts produce three QS signals, one of which is mainly responsible for regulating symbiont colonization of the diatom microenvironment. These findings suggest that QS regulates colonization of diatom surfaces and further work on these model systems will inform our understanding of particle aggregation and bacterial attachment to marine snow and how these processes influence the global carbon cycle.SummaryInteractions between phytoplankton and bacteria play major roles in global biogeochemical cycles and oceanic nutrient fluxes. These interactions occur in the microenvironment surrounding phytoplankton cells, known as the phycosphere. Bacteria in the phycosphere use either chemotaxis or attachment to benefit from algal excretions. Both processes are regulated by quorum sensing (QS), a cell-cell signaling mechanism that uses small infochemicals to coordinate bacterial gene expression. However, the role of QS in regulating bacterial attachment in the phycosphere is not clear. Here, we isolated a Sulfitobacter pseudonitzschiae F5 and a Phaeobacter sp. F10 belonging to the marine Roseobacter group and an Alteromonas macleodii F12 belonging to Alteromonadaceae, from the microbial community of the ubiquitous diatom Asterionellopsis glacialis. We show that only the Roseobacter group isolates (diatom symbionts) can attach to diatom transparent exopolymeric particles. Despite all three bacteria possessing genes involved in motility, chemotaxis, and attachment, only S. pseudonitzschiae F5 and Phaeobacter sp. F10 possessed complete QS systems and could synthesize QS signals. Using UHPLC-MS/MS, we identified three QS molecules produced by both bacteria of which only 3-oxo-C16:1-HSL strongly inhibited bacterial motility and stimulated attachment in the phycosphere. These findings suggest that QS signals enable colonization of the phycosphere by algal symbionts.

show abstract

Acyl‐Homoserine Lactone‐Based Quorum Sensing in Members of the Marine Bacterial Roseobacter Clade: Complex Cell‐to‐Cell Communication Controls Multiple Physiologies

Cited by 12 publications

References 36 publications

Quorum sensing regulates ‘swim‐or‐stick’ lifestyle in the phycosphere

Quorum sensing regulates ‘swim‐or‐stick’ lifestyle in the phycosphere

Extending the “One Strain Many Compounds” (OSMAC) Principle to Marine Microorganisms

Quorum Sensing Regulates ‘swim-or-stick’ Lifestyle in the Phycosphere

Contact Info

Product

Resources

About