Acyl-CoA Synthetase 5 Promotes the Growth and Invasion of Colorectal Cancer Cells

Abstract

Background and Aims Acyl-CoA synthetase 5 (ACS5) has been reported to be associated with the development of various cancers, but the role of it in colorectal cancer (CRC) is not well understood. The present study aimed to explore the potential role of ACS5 in the development and progression of CRC. Methods ACS5 expression in CRC tissues and CRC cell lines was examined, and its clinical significance was analyzed. The role of ACS5 in cell proliferation, apoptosis, and invasion was examined in vitro. Results We found that ACS5 expression was upregulated in CRC cells and CRC tissues and that high ACS5 expression was more frequent in CRC patients with excess muscular layer and with poor tumor differentiation. Furthermore, knockdown of ACS5 in HT29 and SW480 cells significantly dampened cell proliferation, induced cell apoptosis, and reduced cell migration and invasion. In contrast, the ectopic overexpression of ACS5 in LOVO and SW620 cells remarkably promoted cell proliferation, inhibited cell apoptosis, and enhanced cell migration and invasion. Enhanced cell growth and invasion ability mediated by the gain of ACS5 expression were associated with downregulation of caspase-3 and E-cadherin and upregulation of survivin and CD44. Conclusions Our data demonstrate that ACS5 can promote the growth and invasion of CRC cells and provide a potential target for CRC gene therapy.

Lack of glutathione peroxidase-8 in the ER impacts on lipid composition of HeLa cells microsomal membranes

“…It is not inconsistent, therefore, that in HT1080 cells MUFA are activated, instead, by ACSL3 [33]. In more general terms, the upregulation of ACSL5 in siGPx8 HeLa is worth of note also on the light of the emerging role of the ACLS isoforms, and their fatty acid preferences, in different patho-physiological conditions [34,35].…”

Data Independent Acquisition Proteomic Analysis Can Discriminate between Actinic Keratosis, Bowen’s Disease, and Cutaneous Squamous Cell Carcinoma

“…Upregulation of the extracellular matrix protein BGN correlates with poor differentiation in colorectal cancers (Gu et al, 2011) and tumor invasiveness in metastatic melanoma (Andrlová et al, 2017). Overexpression of AACS is linked with increased invasiveness in colorectal (Ding et al, 2017) and renal cell carcinomas (Yao et al, 2018), and increased HP1BP3 is linked with chemoresistant colorectal cancer (Hadac et al, 2016). The ability to accurately discriminate stages of cSCC differentiation has significant implications for clinical management.…”

Targeting the Key Enzymes of Abnormal Fatty Acid β-oxidation as a Potential Strategy for Tumor Therapy

“…ACSL5 has been shown to act as a modifier of Wnt signaling activity in addition to its proapoptotic effect [44]. Its abnormal expression may cause the downregulation of caspase-3 and E-cadherin and the upregulation of survivin and CD44 [45,46]. The above results suggest that ACSLs have the potential to become targets for tumor treatment.…”