Acyl-CoA N-acyltransferase influences fertility by regulating lipid metabolism and jasmonic acid biogenesis in cotton

Fu, Wenfeng; Shen, Ying; Hao, Juan; Wu, Jianyong; Ke, Liping; Wu, Caiyun; Huang, Kai‐Wen; Luo, Binglun; Xu, Mingfeng; Cheng, Xiaofei; Zhou, Xueping; Sun, Jie; Xing, Chaozhu; Sun, Yuqiang

doi:10.1038/srep11790

Cited by 49 publications

(41 citation statements)

References 63 publications

(90 reference statements)

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“…Additional GWAS analyses using reduced datasets revealed other interesting genes for this QTL (chromosome 2A, Supplementary Materials Table S8), encoding for the Acyl-CoA N-acyltransferase and the chloroplastic zeaxanthin epoxidase. The first gene has several functions in signaling and metabolic pathways [115]. The zeaxanthin epoxidase plays an important role in the xanthophyll cycle and abscisic acid (ABA) biosynthesis.…”

Section: Discussionmentioning

confidence: 99%

Mapping Agronomic and Quality Traits in Elite Durum Wheat Lines under Differing Water Regimes

et al. 2020

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Final grain production and quality in durum wheat are affected by biotic and abiotic stresses. The association mapping (AM) approach is useful for dissecting the genetic control of quantitative traits, with the aim of increasing final wheat production under stress conditions. In this study, we used AM analyses to detect quantitative trait loci (QTL) underlying agronomic and quality traits in a collection of 294 elite durum wheat lines from CIMMYT (International Maize and Wheat Improvement Center), grown under different water regimes over four growing seasons. Thirty-seven significant marker-trait associations (MTAs) were detected for sedimentation volume (SV) and thousand kernel weight (TKW), located on chromosomes 1B and 2A, respectively. The QTL loci found were then confirmed with several AM analyses, which revealed 12 sedimentation index (SDS) MTAs and two additional loci for SV (4A) and yellow rust (1B). A candidate gene analysis of the identified genomic regions detected a cluster of 25 genes encoding blue copper proteins in chromosome 1B, with homoeologs in the two durum wheat subgenomes, and an ubiquinone biosynthesis O-methyltransferase gene. On chromosome 2A, several genes related to photosynthetic processes and metabolic pathways were found in proximity to the markers associated with TKW. These results are of potential use for subsequent application in marker-assisted durum wheat-breeding programs.

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Section: Discussionmentioning

confidence: 99%

Mapping Agronomic and Quality Traits in Elite Durum Wheat Lines under Differing Water Regimes

et al. 2020

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“…Auxin and jasmonic acid (JA) are critical regulators during this process: Auxin‐insensitive mutants exhibit early anther dehiscence, whereas JA‐deficient and ‐insensitive mutants show defective or delayed anther dehiscence (Ishiguro et al ., ; Cecchetti et al ., ; Peng et al ., ; Jewell and Browse, ). Even perturbed fatty acid biosynthesis, which provides the JA precursor, linolenic acid, by downregulating fatty acid desaturases (McConn and Browse, ) or acyl‐CoA N‐acyltransferase (Fu et al ., ), leads to delayed anther dehiscence that can be rescued by JA. In addition, perturbation of lignin or flavonoids metabolism and/or transport have been shown to affect anther dehiscence and reduce male fertility (Steiner‐Lange et al ., ; Schilmiller et al ., ; Thompson et al ., ; Ghelli et al ., ).…”

Section: Discussionmentioning

confidence: 99%

Imbalance of tyrosine by modulating TyrA arogenate dehydrogenases impacts growth and development of Arabidopsis thaliana

et al. 2019

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L-Tyrosine is an essential aromatic amino acid required for the synthesis of proteins and a diverse array of plant natural products; however, little is known on how the levels of tyrosine are controlled in planta and linked to overall growth and development. Most plants synthesize tyrosine by TyrA arogenate dehydrogenases, which are strongly feedback-inhibited by tyrosine and encoded by TyrA1 and TyrA2 genes in Arabidopsis thaliana. While TyrA enzymes have been extensively characterized at biochemical levels, their in planta functions remain uncertain. Here we found that TyrA1 suppression reduces seed yield due to impaired anther dehiscence, whereas TyrA2 knockout leads to slow growth with reticulate leaves. The tyra2 mutant phenotypes were exacerbated by TyrA1 suppression and rescued by the expression of TyrA2, TyrA1 or tyrosine feeding. Low-light conditions synchronized the tyra2 and wild-type growth, and ameliorated the tyra2 leaf reticulation. After shifting to normal light, tyra2 transiently decreased tyrosine and subsequently increased aspartate before the appearance of the leaf phenotypes. Overexpression of the deregulated TyrA enzymes led to hyper-accumulation of tyrosine, which was also accompanied by elevated aspartate and reticulate leaves. These results revealed that TyrA1 and TyrA2 have distinct and overlapping functions in flower and leaf development, respectively, and that imbalance of tyrosine, caused by altered TyrA activity and regulation, impacts growth and development of Arabidopsis. The findings provide critical bases for improving the production of tyrosine and its derived natural products, and further elucidating the coordinated metabolic and physiological processes to maintain tyrosine levels in plants.

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“…The new CLCrV-based vector was modified from the CLCrV DNA-A and DNA-B components individually, which were inserted into the pCambia1300 vector to generate pCLCrVA and pCLCrVB, respectively [45]. The fragments of candidate genes were inserted into pCLCrVA to produce pCLCrVA-GhCHS, pCLCrVA-GhANR, pCLCrVA-GhLAR for VIGS in cotton plants as described in the previous papers [45][46][47].…”

Section: Gene Cloning and Construction Of Rnai Vectorsmentioning

confidence: 99%

“…A 400-600 bp fragment of the candidate genes of GhANR2, GhLAR1 and GhCHS2 was amplified as our previous paper [46]. The deficiency of phytoene desaturase gene (PDS) causes loss of chlorophyll and carotenoids, showed the typical photobleaching phenotype, was used as a positive marker to visualize the timing and extent of endogenous gene silencing.…”

Section: Gene Cloning and Construction Of Rnai Vectorsmentioning

confidence: 99%

“…The deficiency of phytoene desaturase gene (PDS) causes loss of chlorophyll and carotenoids, showed the typical photobleaching phenotype, was used as a positive marker to visualize the timing and extent of endogenous gene silencing. The PDS gene fragment was cloned from C312 genomic DNA by PCR to construct vector pCLCrV-GhPDS according to the previous paper [46].…”

Section: Gene Cloning and Construction Of Rnai Vectorsmentioning

confidence: 99%

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Functional analysis of GhCHS, GhANR and GhLAR in colored fiber formation of Gossypium hirsutum L.

Jianfang

Shen

Jingli

et al. 2019

Preprint

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Background The formation of natural colored fibers mainly results from the accumulation of different anthocyanidins and their derivatives in the fibers of Gossypium hirsutum L. Chalcone synthase (CHS) is the first committed enzyme of flavonoid biosynthesis, and anthocyanidins are transported into fiber cell after biosynthesis mainly by Anthocyanidin reductase (ANR) and Leucoanthocyanidin reductase (LAR) to present diverse colors with distinct stability. The biochemical and molecular mechanism of pigment formation in natural colored cotton fiber is not clear. Results The three key genes of GhCHS , GhANR and GhLAR were predominantly expressed in the developing fibers of colored cotton. In the GhCHSi , GhANRi and GhLARi transgenic cottons, the expression levels of GhCHS , GhANR and GhLAR significantly decreased in the developing cotton fiber, negatively correlated with the content of anthocyanidins and the color depth of cotton fiber. In colored cotton Zongxu1 (ZX1) and the GhCHSi , GhANRi and GhLARi transgenic lines of ZX1, HZ and ZH, the anthocyanidin contents of the leaves, cotton kernels, the mixture of fiber and seedcoat were all changed and positively correlated with the fiber color. Conclusion The three genes of GhCHS , GhANR and GhLAR were predominantly expressed early in developing colored cotton fibers and identified to be a key genes of cotton fiber color formation. The expression levels of the three genes affected the anthocyanidin contents and fiber color depth. So the three genes played a crucial part in cotton fiber color formation and has important significant to improve natural colored cotton quality and create new colored cotton germplasm resources by genetic engineering.

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Acyl-CoA N-acyltransferase influences fertility by regulating lipid metabolism and jasmonic acid biogenesis in cotton

Cited by 49 publications

References 63 publications

Mapping Agronomic and Quality Traits in Elite Durum Wheat Lines under Differing Water Regimes

Mapping Agronomic and Quality Traits in Elite Durum Wheat Lines under Differing Water Regimes

Imbalance of tyrosine by modulating TyrA arogenate dehydrogenases impacts growth and development of Arabidopsis thaliana

Functional analysis of GhCHS, GhANR and GhLAR in colored fiber formation of Gossypium hirsutum L.

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