Acute toxicity study of the ethanolic extract of Eleutherine bulbosa Urb in Wistar rats

Abstract

Introduction: Eleutherine bulbosa Urb is a plant species with medicinal properties, including anti-inflammatory, widely relied upon in traditional practices. For this reason, the present research was intended to assess and, thus, ensure the safety of this plant for conventional medicinal purposes using a toxicity test study. Methods: The acute toxicity test of the ethanolic extract of E. bulbosa Urb (EEEB) used the method adopted from the Organization Economic Cooperation and Development (OECD) guidelines 425 for testing Wistar rats. Results: During 14 days of the acute toxicity study, there were no significant changes in rat weight, no mortality, and no signs of toxicity after the oral EEEB administration at 2000 mg/kg body weight (bw). The limit test showed that the LD50 of EEEB was higher than 2000 mg/kg bw. Conclusion: EEEB has low toxicity because its LD50 is higher than the limit test results.

Immunomodulatory and Acute Toxicity Tests of Rhizome Ethanol Extract of Etlingera Flexuosa Poulsen (Zingiberaceae) on Male Mice (Mus Musculus)

“…This means that the increase in body weight of male mice in different groups has almost the same increase. However, there are many factors or variables that affect changes in body weight such as stress, movement space, and feed intake given 41.…”

Anti-Rheumatoid Arthritis Activity of 96% Ethanol Extract of Eleutherine bulbosa Bulbs with Arthritis Induction Adjuvant Method

“…The maserate was evaporated with a rotary evaporator at 40 rpm, 50ºC. The extract was concentrated on a waterbath at 50ºC until a thick extract 17 . Alkaloid tests: As much as 0.5 g of the sample was diluted in 15 mL of distilled water, then 5 mL of HCl was added and filtered.…”

Characterization and determination of total phenol levels of ethanolic extract of bawang dayak bulbs (Eleutherine bulbosa urb.) based on variation in growing time of plants

“…Martantiningtyas et al (2015) menyatakan bahwa senyawa fenol mampu bekerja sebagai antioksidan. Selain itu tanaman ini juga telah diuji keamanannya melalui uji toksisitas metode OECD 425 (Wati et al, 2021), sehingga berpotensi untuk diteliti lebih lanjut.…”