Acute toxicity study of Phyllanthus niruri and its effect on the cyto-architectural structure of nephrocytes in Swiss albino mice Mus-musculus

Singh, T.; Ruchi, Ruchi; Singh, Anjali; Kumar, Ravish; Singh, Jitendra

doi:10.5530/pj.2016.1.17

Cited by 7 publications

(6 citation statements)

References 11 publications

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“…The serum cholesterol level was decreased which directly triggers the activity of lecithin-cholesterol acyl transferase due to the presence of saponin in polyherbal formulation (Bharathi et al, 2011). The serum cholesterol level was reduced due to the increase in activity of lipoprotein lipase and presence of cholesterol-7-hydroxylase, hepatic 3-methyglutaryl-COA reductase, and fatty acid synthetase (Singh et al, 2016;Bharathi et al, 2011). Polyherbal formulation shows the hypolipidemic properties and antioxidant properties which decreases the low-density lipoprotein, total cholesterol, very low-density lipoprotein, and increase the high-density lipoproteins (Parasuraman et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…Regarding the lipid profiling, the results showed that the male rats presented significantly elevated (P < 0.05) serum cholesterol, serum triglycerides, serum HDL cholesterol, and VLDL cholesterol level as compared to female rats. Results are well in accordance with different findings suggesting that male rats showed significant (P < 0.05) increase in serum cholesterol level (15.83 mg dL À1 ), serum triglycerides level (82.70 mg dL À1 ), serum HDL cholesterol level (50.28 mg dL À1 ), and serum HDL cholesterol level (16.54 mg dL À1 ) as compared to female rats (118.30, 78.15, 58.20, 43.33, and 11.64 mg dL À1 respectively) by intake of herbal extract such as Phyllantus niruri, Achyranthes aspera Linn, Platycodon grandiflorum, and Panax ginseng (Drexel, 2006;Barter et al, 2007;Ihedioha et al, 2013;Singh et al, 2016). Igbokwe et al (2009) also reported higher serum HDL cholesterol in male rats (46.35 mg dL À1 ) as compared to female rats (45.20 mg dL À1 ) while decreasing the level of hepatic content.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of the acute basic biological effects of herbal formulation to control obesity: a preliminary study

Kumar,

Kaushik,

Kaushik

et al. 2023

Int J of Food Sci Tech

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SummaryObjectivesObesity is the accumulation of excessive visceral and subcutaneous fat in the body. The herbal formulation can help treat obesity and our study aims to evaluate the acute basic biological effects of herbal formulation to control obesity.MethodsAntiobesity herbal formulation, prepared by the combination of Saussurea lappa root, Betula utilis bark, Aconitum heterophyllum root, Bunium periscum seed, and Bauhinia variegate leaf (4:3:1:1:1), was carried out on Wistar male and female rats. Obesity was induced in the rats by feeding the Chow diet, and the effect of herbal formulation on obesity was studied. The effect on obesity was measured by analyzing variables like weight, water intake, pellet count, hematological parameters, renal function test, liver function test, and lipid profile test.ResultsIn the study, it was observed that the female rats treated at the highest dose showed a significantly (p<0.005) higher decrease in weight compared to male rats after ten days. Thefood intake, hemoglobin, renal, liver function and lipid profile showed significant improvement after the intake of herbal formulation.ConclusionsFrom the above results, it was inferred that herbal formulation increased the activity of metabolism, which is helpful in weight reduction; moreover, it has some beneficial biological effects.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Evaluation of the acute basic biological effects of herbal formulation to control obesity: a preliminary study

Kumar,

Kaushik,

Kaushik

et al. 2023

Int J of Food Sci Tech

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“…A previous study reported that the median acute toxicity (LD50) of PNE administered to Swiss albino mice was 2600 mg/kg, which suggests that PNE may cause mortality in healthy mice ( 23 ). However, in our study, we did not observe any mortality in healthy mice receiving up to 2000 mg/kg dose of PNE after 14 days of administration.…”

Section: Discussionmentioning

confidence: 99%

Suppression of hypoxia and inflammatory pathways by Phyllanthus niruri extract inhibits angiogenesis in DMBA-induced breast cancer mice

et al. 2021

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Background and purpose: Angiogenesis has been one of the hallmarks of cancer. In recent years, Phyllanthus niruri extract (PNE) was reported to inhibit angiogenesis by decreasing the levels of vascular endothelial growth factor (VEGF) and hypoxia-inducible factor-1α (HIF-1α) in breast cancer. However, the experimental results were confirmed in cancer cell lines only, whereas the anti-angiogenic activity in animal models has not been demonstrated. In this study, we tried to examine the anti-angiogenic activity of PNE on BALB/c strain mice models that were induced for breast cancer using the carcinogenic substance 7,12- dimethylbenz[a]anthracene (DMBA). Experimental approach: Experimental animals were divided into five different groups; vehicle, DMBA, PNE 500 mg/kg, PNE 1000 mg/kg; and PNE 2000 mg/kg. Mammary carcinogenesis was induced using a subcutaneous injection of 15 mg/kg of DMBA for 12 weeks. Afterward, oral PNE treatment was given for the following 5 weeks. VEGFA and HIF-1α were observed using immunohistochemistry. Endothelial cell markers CD31, CD146, and CD34 were observed using the fluorescent immunohistochemistry method. The levels of interleukin-6 (IL-6), IL-17, and C-X-C motif chemokine (CXCL12) were measured using flow cytometry. Findings/Results: The survival analysis indicated that PNE increased the survival rate of mice ( P = 0.043, log-rank test) at all doses. The PNE treatment decreased the immunoreactive score of angiogenic factors (VEGF and HIF-1α), as well as the endothelial cell markers (CD31, CD146, and CD34). The PNE- treated groups also decreased the levels of inflammatory cytokines (IL-6, IL-17, and CXCL12) at all doses. Conclusion and implications: This finding suggests that PNE may inhibit the progression of angiogenesis in breast cancer mice by targeting the hypoxia and inflammatory pathways.

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“…The animals were maintained under controlled temperature with 12 h dark cycle and 12 h light cycle. Twenty-four mice were divided into four groups [1][2][3][4] of six animals in each group for the study. Each group contains three male and three female mice.…”

Section: Methodsmentioning

confidence: 99%

“…The utilization of herbal plants to treat various human diseases is common in developing countries due to their easy access and low cost, compared with advanced Western medicine [1]. These herbal formulations were routinely used by humans throughout history, without any scientific evidence, but now are being used in practical clinical trials to find their optimum and beneficial and toxic therapeutic dose ranges [1]. This led to the development of numerous alkaloid drugs such as digitoxin in heart failure patients and anticancer drugs such as vincristine and vinblastine for various cancer [2].…”

Section: Introductionmentioning

confidence: 99%

Neuropharmacological Study of Phyllanthus Niruri in Swiss Albino Mice

Nagaraj

Saravanan

Mani

2021

Asian J Pharm Clin Res

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Objective: The objective of this study is to evaluate the neurological, behavioral, and autonomic changes of Phyllanthus niruri in Swiss albino mice using Irwin’s method. Methods: A total of 24 mice was divided into four groups of six each (3-male, 3-female in each group). Aqueous extract of P. niruri was prepared. Based on body weight aqueous extract was given to the mice by orally through gavage tube (Group I – 300 mg/kg, Group II – 600 mg/kg, Group III – 900 mg/kg, and Group IV – 1200 mg/kg). Neuropharmacological profile is studied for each mice using Irwin’s observational test, the mice were observed for 4 h after oral administration for various behavioral, neurological, and autonomic changes at 0, 1, 2, 3, 4 h. Results: P. niruri showed negligible actions at 300 mg/kg and 600 mg/kg body weight. At 900 mg/kg and 1200 mg/kg P. niruri showed certain behavioral and neuronal changes. P. niruri increased alertness, stereotypy, restlessness, irritability/aggressiveness in behavioral profile indicating that the drug is a CNS stimulant. Furthermore, it showed mild tremors in neurological profile indicating CNS excitation. Conclusion: Aqueous extract of P. niruri at 900 mg/kg and 1200 mg/kg showed changes in behavioral profile, neurological profile, showing it as CNS stimulant properties. Since it is an observational study further research should be done to explore CNS stimulant properties in various in vivo studies.

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Acute toxicity study of Phyllanthus niruri and its effect on the cyto-architectural structure of nephrocytes in Swiss albino mice Mus-musculus

Cited by 7 publications

References 11 publications

Evaluation of the acute basic biological effects of herbal formulation to control obesity: a preliminary study

Evaluation of the acute basic biological effects of herbal formulation to control obesity: a preliminary study

Suppression of hypoxia and inflammatory pathways by Phyllanthus niruri extract inhibits angiogenesis in DMBA-induced breast cancer mice

Neuropharmacological Study of Phyllanthus Niruri in Swiss Albino Mice

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