Acute toxicity screening of different extractions, components and constituents of Polygonum multiflorum Thunb. on zebrafish (Danio rerio) embryos in vivo

Yang, Jian‐Bo; Li, Wanfang; Liu, Yue; Wang, Qi; Cheng, Xian‐Long; Wei, Feng; Wang, Aiguo; Jin, Hongtao; Ma, Shuang‐Cheng

doi:10.1016/j.biopha.2018.01.033

Cited by 47 publications

(35 citation statements)

References 30 publications

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“…The Chinese Pharmacopoeia (2015 edition) stipulates that the dose of RPM is 3-6 g per day, while that of PMP is 3-12 g per day (State pharmacopoeia committee, 2015b). Although the toxicity of PM was rarely mentioned in medical literatures of past dynasties, several reports and information in recent years suggest that excessive and protracted usage of PM can cause toxic reactions in the body Yang et al, 2018). In general, the toxicity induced by a medication is proportional to the dose and the duration of exposure to the drug (Bárándi et al, 2010;Yue et al, 2018).…”

Section: Inappropriate Dosage and Medication Timementioning

confidence: 99%

“…Rhein, emodin and chrysophanol are the main components of anthraquinones in PM, which hepatotoxicity is related to total bilirubin and transaminase contents, inducing liver tissue inflammation, hepatocyte apoptosis and oxidative stress. Yang et al used zebrafish as a model to study the hepatotoxicity of different extracts and different monomer components of PM (7 anthraquinones, 8 terpenoids, 7 anthrones, 3 cinnamic acid amides and 2 naphthol), and showed that the median lethal dose of emodin was the lowest (Yang et al, 2018). In conclusion, more studies are required to unravel the mechanism of toxicity of PM, and toxicity process in the body.…”

Section: Anthraquinonesmentioning

confidence: 99%

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Polygonum multiflorum-Induced Liver Injury: Clinical Characteristics, Risk Factors, Material Basis, Action Mechanism and Current Challenges

et al. 2019

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Polygonum multiflorum Thunb. (PM), called Heshouwu in China, is a popular Chinese medicine in clinical practice. Several clinical studies have been conducted to evaluate the traditional therapeutic claims and to study the potential therapeutic activity of PM in dyslipidemia and neurodegenerative diseases, highlighting available clinical evidence. In recent years, reports on clinical adverse reactions of Raw Radix P. multiflorum (RPM) and P. multiflorum Praeparata (PMP) have been on the increase, especially with respect to liver injury. Most liver injury cases had been assessed for causality using RUCAM (Roussel Uclaf Causality Assessment Method) in this paper. However, the components of PM responsible for the reported hepatotoxic effects have not yet been identified. Moreover, many of the reports are contradictory, while studies on the mechanism involved in PM-induced liver damage are not comprehensive. This study was aimed at reviewing the status of research on liver injury due to PM, including clinical characteristics, risk factors, material basis research and mechanism of action, with a view to understanding PM-induced hepatotoxicity, and taking reasonable and effective measures to prevent it. In short, quality control is still one of the major safety problems in TCM drug safety concerns. The model of safety monitoring and risk management of PM drugs is not yet developed. Indeed, the characteristics and risk factors associated with PM require both proper understanding and control of the risk by strengthening standardization of clinical applications, basic science research, quality control in manufacturing, active monitoring methodology and enhancement of international communication and cooperation. Measures should also be encouraged and implemented to promote healthy development of the TCM industry.

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Section: Inappropriate Dosage and Medication Timementioning

confidence: 99%

Section: Anthraquinonesmentioning

confidence: 99%

Polygonum multiflorum-Induced Liver Injury: Clinical Characteristics, Risk Factors, Material Basis, Action Mechanism and Current Challenges

et al. 2019

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“…Actually, hundreds of compounds have been isolated from PM with different processing procedures that alter the properties and functions of remedies; so, a standardized processing procedure may be favorable for managing PM use. In acute toxicity screening using zebrafish embryos, PM extracted with different concentrations of an ethanolic solvent showed decreasing toxicity for 70% ≅ 95% > 50% > 30% > water [ 10 ]. When considering the solubility of active constituents, PM extracted with 50% ethanol (PME) retained its therapeutic activities while reducing potential toxicities.…”

Section: Introductionmentioning

confidence: 99%

Hepatoprotective Activity of the Ethanolic Extract of Polygonum multiflorum Thunb. against Oxidative Stress-Induced Liver Injury

Lin

Chagnaadorj

Huang

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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Oxidative stress is an important pathological mechanism in various liver diseases. Polygonum multiflorum Thunb. (PM) can be used for the treatment of diseases associated with aging, hyperlipidemia, and oxidative stress in traditional Chinese medicine. In this study, we examined the hepatoprotective effects of the ethanolic extract of PM (PME) in in vitro and in vivo models. The PME induced expression of antioxidant-response-element- (ARE-) related genes in HepG2 cells showed a dose-dependent manner. Pretreatment of HepG2 cell with PME suppressed H2O2- and acetaminophen- (APAP-) induced cellular reactive oxygen species (ROS) generation and cytotoxicity. In APAP-induced mouse liver injury, pretreatment with PME also showed ability to increase the survival rate and reduce the severity of liver injury. Treatment with PME attenuated bile duct ligation-induced extrahepatic cholestatic liver injury and further increased multidrug resistance protein 4 (MRP4) and reduced organic anion-transporting polypeptide (OATP) expression. Furthermore, increased nuclear translocation of the nuclear factor erythroid 2-related factor 2 (Nrf2) was observed after treatment with PME in both in vivo models. In conclusion, the current study showed the hepatoprotective activity of PME by regulating the redox state in liver injury through Nrf2 activation and controlling hepatic bile acid homeostasis in obstructive cholestasis, through bile acid transporter expression modulation.

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“…Therefore, it was not recommended to make wine with PMR for nourishing. 70% ethanol extract had the highest toxicity [140]. Studies showed that the occurrence of adverse reactions was related to time and dose, and a long-term large dose was more likely to cause hepatotoxicity [139].…”

Section: Knowledge Of Toxicitymentioning

confidence: 99%

A Candidate Drug for Nonalcoholic Fatty Liver Disease: A Review of Pharmacological Activities of Polygoni Multiflori Radix

Zhou

Liu

et al. 2020

BioMed Research International

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Nonalcoholic fatty liver disease, a type of metabolic syndrome, continues to rise globally. Currently, there is no approved drug for its treatment. Improving lifestyle and exercise can alleviate symptoms, but patients’ compliance is poor. More and more studies have shown the potential of Polygoni Multiflori Radix (PMR) in the treatment of NAFLD and metabolic syndrome. Therefore, this paper reviews the pharmacological effects of PMR and its main chemical components (tetrahydroxystilbene glucoside, emodin, and resveratrol) on NAFLD. PMR can inhibit the production of fatty acids and promote the decomposition of triglycerides, reduce inflammation, and inhibit the occurrence of liver fibrosis. At the same time, it maintains an oxidation equilibrium status in the body, to achieve the therapeutic purpose of NAFLD and metabolic syndrome. Although more standardized studies and clinical trials are needed to confirm its efficacy, PMR may be a potential drug for the treatment of NAFLD and its complications. However, the occurrence of adverse reactions of PMR has affected its extensive clinical application. Therefore, it is necessary to further study its toxicity mechanism, enhance efficacy and control toxicity, and even reduce toxicity, which will contribute to the safe clinical use of PMR.

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Acute toxicity screening of different extractions, components and constituents of Polygonum multiflorum Thunb. on zebrafish (Danio rerio) embryos in vivo

Cited by 47 publications

References 30 publications

Polygonum multiflorum-Induced Liver Injury: Clinical Characteristics, Risk Factors, Material Basis, Action Mechanism and Current Challenges

Polygonum multiflorum-Induced Liver Injury: Clinical Characteristics, Risk Factors, Material Basis, Action Mechanism and Current Challenges

Hepatoprotective Activity of the Ethanolic Extract of Polygonum multiflorum Thunb. against Oxidative Stress-Induced Liver Injury

A Candidate Drug for Nonalcoholic Fatty Liver Disease: A Review of Pharmacological Activities of Polygoni Multiflori Radix

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