Acute toxicity of Zinc pyrithione to human skin cells in vitro

Priestley, G. C.; Brown, Jayann

doi:10.2340/0001555560145148

Cited by 23 publications

(2 citation statements)

References 11 publications

(15 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Effects of 30 μM ZPT + ciprofloxacin suggest greater efficiency of killing at higher ciprofloxacin concentrations, yet no change in MIC from ciprofloxacin‐only plates. Use of ZPT at such high concentrations could be unwise as 10 μM ZPT has shown toxicity in human cells (Priestley & Brown, 1980). Twenty‐five micromolar FePcTs has previously been shown to potentiate ciprofloxacin effects, our results did now show any potentiation or sensitivity effects.…”

Section: Discussionmentioning

confidence: 99%

“…Effects of 30 μM ZPT + ciprofloxacin suggest greater efficiency of killing at higher ciprofloxacin concentrations, yet no change in MIC from ciprofloxacin-only plates. Use of ZPT at such high concentrations could be unwise as 10 μM ZPT has shown toxicity in human cells (Priestley & Brown, 1980 F I G U R E A5 ML328 (a) and IMP-1700 (b) OD 600 and IC 50 linear regression data for Escherichia coli strains (i and ii) MG1655 (WT; wild-type), (iii and iv) ΔrecA::Kan R (HH020), (v and vi) ΔrecB::Kan R (EAW102). The optical density at 600 nm (OD 600 ) was recorded every 20 min for 18 h. OD 600 measurements were background corrected against noinoculum controls.…”

Section: Development Of Dsbr Inhibitors: the Challenge Of Off-target ...mentioning

confidence: 99%

See 1 more Smart Citation

Defects in DNA double‐strand break repair resensitize antibiotic‐resistant Escherichia coli to multiple bactericidal antibiotics

et al. 2022

View full text Add to dashboard Cite

Antibiotic resistance is becoming increasingly prevalent amongst bacterial pathogens and there is an urgent need to develop new types of antibiotics with novel modes of action. One promising strategy is to develop resistance‐breaker compounds, which inhibit resistance mechanisms and thus resensitize bacteria to existing antibiotics. In the current study, we identify bacterial DNA double‐strand break repair as a promising target for the development of resistance‐breaking co‐therapies. We examined genetic variants of Escherichia coli that combined antibiotic‐resistance determinants with DNA repair defects. We observed that defects in the double‐strand break repair pathway led to significant resensitization toward five bactericidal antibiotics representing different functional classes. Effects ranged from partial to full resensitization. For ciprofloxacin and nitrofurantoin, sensitization manifested as a reduction in the minimum inhibitory concentration. For kanamycin and trimethoprim, sensitivity manifested through increased rates of killing at high antibiotic concentrations. For ampicillin, repair defects dramatically reduced antibiotic tolerance. Ciprofloxacin, nitrofurantoin, and trimethoprim induce the promutagenic SOS response. Disruption of double‐strand break repair strongly dampened the induction of SOS by these antibiotics. Our findings suggest that if break‐repair inhibitors can be developed they could resensitize antibiotic‐resistant bacteria to multiple classes of existing antibiotics and may suppress the development of de novo antibiotic‐resistance mutations.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Development Of Dsbr Inhibitors: the Challenge Of Off-target ...mentioning

confidence: 99%

Defects in DNA double‐strand break repair resensitize antibiotic‐resistant Escherichia coli to multiple bactericidal antibiotics

et al. 2022

View full text Add to dashboard Cite

show abstract

Skin Fibroblast Monolayers: A Suitable Case for Treatment?

Priestley¹

1986

Skin Models

View full text Add to dashboard Cite

Contraction of collagen lattices by skin fibroblasts: drug-induced changes

Adams

Priestley

1988

Arch Dermatol Res

View full text Add to dashboard Cite

A range of dermatologically useful drugs were added to human skin fibroblasts cultured in collagen lattices to assess possible effects on the rate of lattice contraction. Vitamin C, Vitamin E, phenytoin, sodium salicylate, D-penicillamine and dibutyryl c-AMP had no significant effect. Chlorhexidine acetate at 10 micrograms/ml arrested contraction after 24 h but this was related to its cytotoxicity. The antibiotics griseofulvin (2-16 micrograms/ml) and cycloheximide (5-30 micrograms/ml) caused dose-related inhibitions of contraction without affecting fibroblast viability. Four corticosteroids at 10 micrograms/ml inhibited contraction, clobetasol propionate having the greatest effect. On the other hand 4 retinoids at 10(-5) M enhanced contraction by up to 20%. As lattice contraction appears to model the contraction of skin wounds and there are broad parallels between the effects shown here of antiseptics, corticosteroids and the retinoids, and their reported influence on healing wounds, the lattice system may be a useful pharmacological screen for new compounds.

show abstract

Acute toxicity of Zinc pyrithione to human skin cells in vitro

Abstract: Zinc pyrithione introduced into cultures of rapidly proliferating NCTC 2544 human skin epithelial cells and normal human skin fibroblasts had a rapid cytotoxic effect even at very low concentrations (0.1-0.5 microgram/ml)

Cited by 23 publications

References 11 publications

Defects in DNA double‐strand break repair resensitize antibiotic‐resistant Escherichia coli to multiple bactericidal antibiotics

Defects in DNA double‐strand break repair resensitize antibiotic‐resistant Escherichia coli to multiple bactericidal antibiotics

Skin Fibroblast Monolayers: A Suitable Case for Treatment?

Contraction of collagen lattices by skin fibroblasts: drug-induced changes

Contact Info

Product

Resources

About