Acute toxicity of sublethal concentrations of thiacloprid and clothianidin to immune response and oxidative status of honey bees

Orčić, Snežana; Čelić, Tatjana V.; Purać, Jelena; Vukašinović, Elvira L.; Kojić, Danijela

doi:10.1007/s13592-022-00959-w

Cited by 9 publications

(4 citation statements)

References 64 publications

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“…Thiacloprid is detoxified by CYP9Q3 in honeybees ( Manjon et al, 2018 ) and bumblebees ( Troczka et al, 2019 ) as well as by CYP9BU in Osmia bicornis solitary bees. However, despite its comparatively lower toxicity, exposure to thiacloprid has shown detrimental effects on honeybees, including reduced survival rates ( Liu et al, 2019 ), decreased immunity ( Shi et al, 2018 ; Mollmann and Colgan, 2022 ; Orčić et al, 2022 ), impaired learning and memory, impaired foraging and homing behavior ( Fent et al, 2020 ; Begna and Jung, 2021 ), altered circadian and sleep patterns ( Tasman et al, 2021 ), damaged spermatogenesis ( Hartman et al, 2021 ), interactions with Nosema ceranae and black queen cell virus (BQCV) ( Doublet et al, 2015 ), and changes in social communications ( Tison et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Chronic larval exposure to thiacloprid impairs honeybee antennal selectivity, learning and memory performances

Chen

Dai

et al. 2023

Front. Physiol.

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The use of agricultural neonicotinoid insecticides has sub-lethal chronic effects on bees that are more prevalent than acute toxicity. Among these insecticides, thiacloprid, a commonly used compound with low toxicity, has attracted significant attention due to its potential impact on the olfactory and learning abilities of honeybees. The effect of sub-lethal larval exposure to thiacloprid on the antennal activity of adult honeybees (Apis mellifera L.) is not yet fully understood. To address this knowledge gap, laboratory-based experiments were conducted in which honeybee larvae were administered thiacloprid (0.5 mg/L and 1.0 mg/L). Using electroantennography (EAG), the impacts of thiacloprid exposure on the antennal selectivity to common floral volatiles were evaluated. Additionally, the effects of sub-lethal exposure on odor-related learning and memory were also assessed. The results of this study reveal, for the first time, that sub-lethal larval exposure to thiacloprid decreased honeybee antenna EAG responses to floral scents, leading to increased olfactory selectivity in the high-dose (1.0 mg/L) group compared to the control group (0 mg/L vs. 1.0 mg/L: p = 0.042). The results also suggest that thiacloprid negatively affected odor-associated paired learning acquisition, as well as medium-term (1 h) (0 mg/L vs. 1.0 mg/L: p = 0.019) and long-term memory (24 h) (0 mg/L vs. 1.0 mg/L: p = 0.037) in adult honeybees. EAG amplitudes were dramatically reduced following R-linalool paired olfactory training (0 mg/L vs. 1.0 mg/L: p = 0.001; 0 mg/L vs. 0.5 mg/L: p = 0.027), while antennal activities only differed significantly in the control between paired and unpaired groups. Our results indicated that exposure to sub-lethal concentrations of thiacloprid may affect olfactory perception and learning and memory behaviors in honeybees. These findings have important implications for the safe use of agrochemicals in the environment.

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Section: Introductionmentioning

confidence: 99%

Chronic larval exposure to thiacloprid impairs honeybee antennal selectivity, learning and memory performances

Chen

Dai

et al. 2023

Front. Physiol.

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“…When pesticides induced oxidative stress in honey bee, reactive oxygen species (ROS) are generated with P450-mediated detoxification processes. Glutaredoxins act as immediate electron donor and its contribution to providing resistance to oxidative stress during honeybee detoxification processes has been well described ( Xu et al, 2013 ; Yao et al, 2014 ; Orčić et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Exposure of chlorothalonil and acetamiprid reduce the survival and cause multiple internal disturbances in Apis mellifera larvae reared in vitro

Lü

Gao²,

Wu³

et al. 2023

Front. Physiol.

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Background: Chlorothalonil and acetamiprid are chemical pesticides commonly used in agricultural production and have been shown to have negative effects on bee’s fitness. Despite many studies have revealed that honey bee (Apis mellifera L.) larvae are posting a high risk on exposure to pesticides, but the toxicology information of chlorothalonil and acetamiprid on bee larvae remain limited.Results: The no observed adverse effect concentration (NOAEC) of chlorothalonil and acetamiprid for honey bee larvae were 4 μg/mL and 2 μg/mL, respectively. Except for CarE, the enzymic activities of GST and P450 were not influenced by chlorothalonil at NOAEC, while chronic exposure to acetamiprid slightly increased the activities of the three tested enzymes at NOAEC. Further, the exposed larvae showed significantly higher expression of genes involved in a series of different toxicologically relevant process following, including caste development (Tor (GB44905), InR-2 (GB55425), Hr4 (GB47037), Ac3 (GB11637) and ILP-2 (GB10174)), immune system response (abaecin (GB18323), defensin-1 (GB19392), toll-X4 (GB50418)), and oxidative stress response (P450, GSH, GST, CarE).Conclusion: Our results suggest that the exposure to chlorothalonil and acetamiprid, even at concentrations below the NOAEC, showed potentially effects on bee larvae’s fitness, and more important synergistic and behavioral effects that can affect larvae fitness should be explored in the further.

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“…According to Yao et al [20], experiments have shown that treatment with clothianidin has minimal impact on the survival rate of adult bees. Orcic et al [34] also suggested a tendency for chronic toxic responses in honey bees, such as weakening their immune system, rather than acute toxicity. However, clothianidin is relatively well-known for its high toxicity, with a 24 h lethal dose 50% (LD50) of 22 ng bee −1 , even among neonicotinoid pesticides [35].…”

Section: Discussionmentioning

confidence: 99%

Effects of Clothianidin Pesticide Application on the Strength of Honey Bee Colonies and Stress-Related Genes in the Vicinity of Rice Fields in the Republic of Korea

Son,

Kim,

Kim

et al. 2023

Diversity

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The honey bee, a crucial organism that provides ecological and economic value to humans, is currently facing threats from various environmental factors including pesticides. Numerous studies have been conducted to demonstrate the risks associated with neonicotinoid pesticides, but research on their occurrence in actual field conditions has not been identified. Therefore, in this study, we observed changes in honey bee (Apis mellifera) colonies located near rice cultivation areas as they progressed beyond the rice pesticide application period. Furthermore, we collected honey bees exposed to the clothianidin and analyzed their stress-related gene expression. The results showed that the foraging behavior of honey bee colonies located near rice cultivation areas did not exhibit significant differences between the treatment sites (Cheongyang and Gimje) and the control site (Wanju) during the experimental period. However, it was observed that the expression levels of stress-related genes in honey bees collected from the treatment group were significantly higher than those in the control. Most of the stress-related genes were associated with detoxification processes in response to pesticides. As a result, pesticide treatment in proximity to rice cultivation areas did not cause direct damage to honey bees but had an indirect impact, suggesting the potential for ongoing chronic damage.

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Acute toxicity of sublethal concentrations of thiacloprid and clothianidin to immune response and oxidative status of honey bees

Cited by 9 publications

References 64 publications

Chronic larval exposure to thiacloprid impairs honeybee antennal selectivity, learning and memory performances

Chronic larval exposure to thiacloprid impairs honeybee antennal selectivity, learning and memory performances

Exposure of chlorothalonil and acetamiprid reduce the survival and cause multiple internal disturbances in Apis mellifera larvae reared in vitro

Effects of Clothianidin Pesticide Application on the Strength of Honey Bee Colonies and Stress-Related Genes in the Vicinity of Rice Fields in the Republic of Korea

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