Acute toxicity of selected metals and phenols on RTG-2 and CHSE-214 fish cell lines

Castaño, Argelia; Vega, Milagros; Tarazona, José V.

doi:10.1007/bf00203013

Cited by 27 publications

(12 citation statements)

References 7 publications

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“…This is consistent with previous work where EC50 values in the NR assay were equally or more sensitive than the KBP assay (Castaño et al, 1995). Due to the wide dose range tested (74-0.03 mL/ 100 mL), samples A (0.03-18 mL/100 mL), 1 (0.03-0.29 mL/100 mL) and 2 (0.03-0.29 mL/100 mL) revealed hormesis characteristics (Figs.…”

Section: Introductionsupporting

confidence: 93%

Short communication. An in vitro approach for ecotoxicity testing of toxic and hazardous wastes

Babín¹,

Cañas²,

Tarazona³

2008

Span. j. agric. res.

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In waste-characterization, chemicals with a low acute toxicity but with a high long-term hazard such as PBTs (persistent, bioaccumulable, and toxic chemicals) are difficult to identify, leading to an inaccurate assessment of their risk to the environment. This study assesses the sensitivity of in vitro assays with rainbow trout cell line RTG-2 as a tool for the ecotoxicological testing of hazardous wastes using a battery of bioassays, including a neutral red (NR) assay for predicting cytotoxicity, a β-galactosidase (βgal) assay for predicting cellular defence, and two sub-lethal damage assays: ethoxy resorufin-O-deethylase (EROD) and glutathione S-transferase (GST) to check for induction of cytochrome and for alteration of antioxidant defences respectively. Six samples of complex waste, covering a wide range of liquid (A to C) and solid (1 to 3) matrices were tested. The EC50-values (NR) ranged from 0.09 mL/100 mL (B) to 2.01 mL/100 mL (2). A hormesis effect (βgal) at low concentrations was observed for the A (0.03-0.18 mL/100 mL), 1 (0.03-0.29 mL/100 mL) and 2 (0.03-0.29 mL/100 mL) samples. A dose-dependent stimulation of enzymatic activity was observed for the B [(EROD (0.58-4.68 mL/100 mL), βgal (0.58-4.68) and GST (0.14-1.17 mL/100 mL)] and C [(EROD (0.29-4.68 mL/100 mL) and βgal (2.34-4.68)] samples. No effects were observed at the highest concentration tested (74 mL/100 mL) for sample 3. The in vitro approach can be considered to be an efficient, rapid and cost effective screening system to provide basic information on toxic and hazardous waste for further analysis and risk evaluation.Additional key words: bioassays, industrial wastes, risk evaluation, RTG-2. ResumenComunicación corta. Aproximación in vitro en el análisis de ecotoxicidad de residuos tóxicos y peligrosos La caracterización de residuos se complica cuando contienen PBTs (sustancias tóxicas persistentes y bioacumulables) y sustancias con baja toxicidad aguda y mucha peligrosidad crónica, impidiendo la evaluación del riesgo ambiental. Este trabajo utiliza la línea celular de trucha RTG-2 para estudiar la sensibilidad de los ensayos in vitro, como herramienta en la evaluación ecotoxicológica de residuos peligrosos. La batería de bioensayos incluye la medida de citotoxicidad mediante el test de rojo neutro (RN), de protección celular mediante la actividad β-galactosidasa (βgal) y la medida de la alteración del citocromo y de las defensas antioxidantes mediante las actividades etoxi resorufin-O-dietilasa (EROD) y glutation S-transferasa (GST) respectivamente. Se ensayaron tres residuos líquidos (A a C) y tres sólidos (1 a 3) representando un amplio rango de muestras. Los valores de EC50 (RN) variaron entre 0,09 mL/100 mL (B) y 2,01 mL/100 mL (2). Se detectaron fenómenos de hormesis (βgal) a bajas concentraciones en las muestras A (0,03-0,18 mL/100 mL), 1 (0,03-0,29 mL/100 mL) y 2 (0,03-0,29 mL/100 mL). Se detectó aumento de las actividades enzimáticas en las muestras B [(EROD (0,58-4,68 mL/100 mL), βgal (0,58-4,68 mL/100 mL) y GST (0,14-1,...

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Section: Introductionsupporting

confidence: 93%

Short communication. An in vitro approach for ecotoxicity testing of toxic and hazardous wastes

Babín¹,

Cañas²,

Tarazona³

2008

Span. j. agric. res.

Self Cite

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“…Cytotoxicity was found in several studies to be correlated with in vivo acute fish toxicity (Castano et al, 1995;Segner, 1998) in different cell lines, which was also found for organotins. Moreover, cytotoxicity of organotins was correlated to the octanol-water partition coefficient (Brüschweiler et al, 1995;Fent, 1996).…”

Section: Ecotoxicitymentioning

confidence: 86%

Ecotoxicological effects at contaminated sites

Fent

2004

Toxicology

152

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“…Cell lines have been used extensively in mammalian (Hsie et al, 1984;Kaji et al, 1992;Bylander et al, 1994) and fish toxicology (Rachlni and Perlmutter, 1968;Kocan et al, 1979;Marion and Denizeau, 1983a,b;Mitani and Egami, 1984;Walton et al, 1984;Bols et al, 1985;Babich and Borenfreund, 1991;Martin-Alguacil et al, 1991;Castafio et al, 1995). The toxicity to established cell lines can be established readily using clonogenic assays (Puck and Marcus, 1955) but may not give relevant information concerning tissue effects or toxic effects involving differentiated characteristics (Doyle et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

The differential effects of cadmium exposure on the growth and survival of primary and established cells from fish and mammals

Lyons-Alcantara¹,

Tarazona²,

Mothersill³

1996

Cell Biol Toxicol

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The differential cytotoxic effects of cadmium on fish and mammalian epithelial cells in established and primary culture were assessed by looking at the reduction of the colony-forming ability and reduction in the extent of growth. The influence of medium composition on the toxicity of cadmium was also studied using serum-free and serum-containing media. The experiments using immortalized cell lines showed that mammalian cells were more sensitive than fish cells to cadmium. Both cell types were grown at the same serum concentration. However, using the normal primary system, human epithelial tissue explants showed less sensitivity to cadmium than did similar cultures from rainbow trout. It is likely that cellular mechanisms of cadmium resistance in the different cell types are responsible for these effects. As expected, cadmium proved to be more toxic when tested in serum-free medium. With fish skin primary cultures, reduction of cell numbers was observed at concentrations as low as 5 mumol/L in serum-free medium compared to 100 mumol/L in serum-containing medium. This was found to be due to the reduction in the activity of free cadmium ions, caused by the presence of serum in the medium. It is concluded that serum-free formulations are probably preferable when testing pollutants in vitro. The results highlight the importance of establishing species-specific pollution tests and standardizing variables. In summary, the results show species and culture media differences in cadmium toxicity and suggest that caution is required when extrapolating results for pollutant effects from one in vitro system to another.

show abstract

Acute toxicity of selected metals and phenols on RTG-2 and CHSE-214 fish cell lines

Cited by 27 publications

References 7 publications

Short communication. An in vitro approach for ecotoxicity testing of toxic and hazardous wastes

Short communication. An in vitro approach for ecotoxicity testing of toxic and hazardous wastes

Ecotoxicological effects at contaminated sites

The differential effects of cadmium exposure on the growth and survival of primary and established cells from fish and mammals

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