Acute Toxicity of LAS Homologues in Marine Microalgae: Esterase Activity and Inhibition Growth as Endpoints of Toxicity

Hampel, Miriam; Moreno-Garrido, Ignácio; Sobrino, Cristina; Lubián, Luís M.; Blasco, Julián

doi:10.1006/eesa.2000.2028

Cited by 73 publications

(37 citation statements)

References 29 publications

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“…This is demonstrated in our results by the lower esterase activity values under high CO 2 compared to low CO 2 conditions, and lower values under high nutrient compared to low nutrient conditions. It is also important to note that the reduced esterase response for downregulated healthy cells differs from that observed in toxicological studies where a decrease in esterase activity is related to the impairment of cell metabolism due to acute damage (Hampel et al 2001, Hadjoudja et al 2009, Eigemann et al 2013. Low light conditions in our study were already within the range of saturating irradiances, while high light conditions were potentially photoinhibitory at midday since the LDPE cubitainers were UVR-transparent.…”

Section: Metabolic Activity and Cell Damagementioning

confidence: 96%

Effect of CO2, nutrients and light on coastal plankton. IV. Physiological responses

Sobrino¹,

Segovia²,

Neale³

et al. 2014

Aquat. Biol.

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We studied the physiological response of phytoplankton to the interacting effects of 3 factors affected by global climate change: CO 2 , nutrient loading and irradiance. Treatments had a high and low level for each factor: CO 2 was bubbled at 1000 ppm by volume versus present atmo spheric values; high nutrient treatments had a combination of inorganic and organic nutrients; and light treatments were obtained by covering the tanks with a single or double layer of screen. We measured esterase activity, oxidative stress (ROS), cell death, DNA damage, photosynthetic efficiency and 14 C assimilation as particulate or dissolved organic material (POC and DOC respectively). Conditions simulating future global change scenarios showed similar chlorophyllnormalized primary productivity as present conditions. The main effect driving phytoplankton physiology was the downregulation of the photosynthetic apparatus by elevated CO 2 , which decreased esterase activity, ROS, cell death and DNA damage. Nutrient concentration and light acted as additional modulators, upregulating or contributing to downregulation. The percentage of DO 14 C extracellular release (PER) was low (0 to 27%), significantly lower under ultraviolet radiation (UVR) than under photosynthetically active radiation (PAR), and acted mainly to reequilibrate the internal balance when cells grown under UVR were exposed to PAR. PER was almost 3 times lower under high CO 2 , confirming a higher resource use efficiency of phytoplankton under future CO 2 concentrations.

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Section: Metabolic Activity and Cell Damagementioning

confidence: 96%

Effect of CO2, nutrients and light on coastal plankton. IV. Physiological responses

Sobrino¹,

Segovia²,

Neale³

et al. 2014

Aquat. Biol.

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“…Biodegradation is regarded as the major pathway to eliminate LAS in the water (Takada and Ogura 1992), but in anoxic and anaerobic conditions, their degradation is very slow and can be accumulated in aquatic environment (Tabor and Barber 1996;González-Mazo et al 1998). LAS show ecotoxicological effects on aquatic organisms (Lewis 1990;Liu et al 2001;Moreno-Garrido et al 2001;Hampel et al 2001;Maenpaa and Kukkonen 2006;Ying 2006;Hodges et al 2006), and they are classified as the second grade contaminant in aquatic ecosystem in China. Therefore, the environmental fate of LAS has been concerned for many years, the distribution of LAS and sulfophenyl carboxylates (mono-and dicarboxylic sulfophenyl acids, the intermediate degradation products of LAS) in the water or sediments has been studied extensively (González-Mazo et al 1997bLeón et al 1999;Matthijs et al 1999;Isobe et al 2004;Li et al 2005), and biodegradation of LAS has also been examined (Krueger et al 1998;Quiroga et al 1999;Perales et al 1999Perales et al , 2007Sanz et al 2003;García et al 2005;Khleifat 2006).…”

Section: Introductionmentioning

confidence: 99%

Linear alkylbenzene sulfonate (LAS) in water of Lake Dianchi—spatial and seasonal variation, and kinetics of biodegradation

Zhi

Xiao

et al. 2010

Environ Monit Assess

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For the purpose of elucidating the environmental characteristics of anion surfactants [linear alkylbenzene sulfonates (LAS)] in the water of Lake Dianchi, a sampling investigation in the field from October 2006 to July 2007 and a batch of biodegradation experiments in the laboratory were carried out. The results showed that concentrations of LAS ranged from 18.6 to 260.1 μg L −1 , and the average concentration was 52.6 μg L −1 in Lake Dianchi. The highest concentrations of LAS were found in the Northwest region, and the distribution of LAS varied through seasons, with much lower concentrations in fall than in any other seasons. LAS can be primarily biodegraded by microorganisms in the lake water, and the per- centage of degradation was >97% after 28 days. Biodegradation of LAS was well-fitted with the kinetic model presented in this paper, and the half-lives ranged from 3.2 to 16.3 days. Temperature, pH, and initial concentrations could affect the biodegradation of LAS, among which temperature was the major factor influencing on biodegradation of LAS in water of Lake Dianchi.

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“…The technique enables the physical and physiological assessment of individual cells within a population (Regel et al 2004). Fluorescein diacetate (FDA) has been recognized as an ideal probe to assess esterase activity (Blaise and Menard 1998, Hampel et al 2001, Lage et al 2001 a Author for correspondence: fxkong@niglas.ac.cn (F.X. Kong) 2002, Adams andStauber 2004).…”

Section: Introductionmentioning

confidence: 99%

Differences in Responses to Darkness betweenMicrocystis aeruginosaandChlorella pyrenoidosa

Zhang

Kong

Shi

et al. 2007

Journal of Freshwater Ecology

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Acute Toxicity of LAS Homologues in Marine Microalgae: Esterase Activity and Inhibition Growth as Endpoints of Toxicity

Cited by 73 publications

References 29 publications

Effect of CO2, nutrients and light on coastal plankton. IV. Physiological responses

Effect of CO2, nutrients and light on coastal plankton. IV. Physiological responses

Linear alkylbenzene sulfonate (LAS) in water of Lake Dianchi—spatial and seasonal variation, and kinetics of biodegradation

Differences in Responses to Darkness betweenMicrocystis aeruginosaandChlorella pyrenoidosa

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