Acute toxicity of 353-nonylphenol and its metabolites for zebrafish embryos

Kammann, Ulrike; Vobach, Michael; Wosniok, Werner; Schäffer, Andreas; Telscher, Markus

doi:10.1007/s11356-009-0123-7

Cited by 3 publications

(3 citation statements)

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“…The 24‐h NOEC of BPA for all sublethal endpoints in zebrafish embryos is 2.0 mg/L . The NOEC of NP for nonlethal malformations in zebrafish embryos is from 1 mg/L to 2.5 mg/L . Consistent with previous studies, our results showed that the exposure did not significantly affect the hatching and survival rates of embryos or larvae.…”

Section: Discussionsupporting

confidence: 91%

The impact of endocrine‐disrupting chemicals on oxidative stress and innate immune response in zebrafish embryos

Yang

Qiu

et al. 2013

Enviro Toxic and Chemistry

126

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Bisphenol A (BPA) and nonylphenol (NP) are well known endocrine-disrupting chemicals (EDCs) ubiquitous in the aquatic environment and are an ecotoxicological risk for the health of aquatic organisms. Limited attention has been given to the immunotoxicity of these chemicals. The present study revealed a concentration-dependent increase of reactive oxygen species content and an induced expression of redox-sensitive transcription factors in zebrafish embryos after exposure to various concentrations of BPA, NP, and BPA/NP mixture for 4 h to 168 h postfertilization. Transcription of genes related to the immune response, including IFNγ, IL1β, IL10, Mx, TNFα, CC-chemokine, and CXCL-clc, were significantly up-regulated on exposure to EDCs. A significant induction of concentrations of proinflammatory mediator, nitric oxide, accompanied by an increase in the activity of nitric oxide synthase (NOS) and an upregulation of inducible NOS gene expression, was detected in zebrafish embryos on exposures to EDCs. To elucidate the potential mechanisms by which BPA and NP activate the innate immune response, expression profiles of genes related to the Toll-like receptors (TLRs) signaling pathway were examined. Expressions of TLR3, TRIF, MyD88, SARM, IRAK4, and TRAF6 were altered on exposure to EDCs. The authors' results demonstrate that exposure to BPA and NP significantly affects the expression of genes related to immune response in zebrafish embryos following oxidative stress.

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Section: Discussionsupporting

confidence: 91%

The impact of endocrine‐disrupting chemicals on oxidative stress and innate immune response in zebrafish embryos

Yang

Qiu

et al. 2013

Enviro Toxic and Chemistry

126

View full text Add to dashboard Cite

show abstract

“…It can be determined in short examinations prior to experiments. To distinguish already low contaminant effects from background mortality, we propose a correspondingly low spontaneous lethality rate (≤2.5%), which is in line with the results from Kammann et al [40,41]. In comparison, the OECD recommends to discard datasets with a lethality equal or above 10% under control conditions [18,19].…”

Section: Recommendations For the Use Of Microinjections As Tool In Tosupporting

confidence: 54%

Microinjection into zebrafish embryos (Danio rerio) - a useful tool in aquatic toxicity testing?

Schubert¹,

Keddig²,

Hanel³

et al. 2014

Environ Sci Eur

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Background: Microinjection was tested as a potentially powerful tool to introduce natural and anthropogenic pollutants directly into fish eggs to determine their toxicological impact on fish. With this technique, parental transfer of lipophilic contaminants may be mimicked. Here, we investigated the applicability of pollutant injection into the yolk of early zebrafish (Danio rerio) eggs with special regard on survival after vehicle injection. Tested vehicles were autoclaved tap water, dimethyl sulfoxide (DMSO), methanol, and triolein. Results: Highest mortality occurred after the injection of DMSO and methanol. The lethality rates were up to 40% higher than under control conditions. Best survival rates were obtained after triolein and water injections. However, the triolein droplet was not assimilated by the embryo within 96 h post fertilization suggesting an incomplete uptake of triolein-solubilized chemicals. Technical aspects concerning microinjection in zebrafish eggs are discussed with special emphasis on quantitative injection. Conclusions: Microinjection into the yolk cell of zebrafish eggs is feasible, but the application of exact volumes appears problematic. However, microinjection is a powerful tool for studies without the demand for high volume accuracy. Adopting microinjection for pollutant research requires further investigation.

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“…For the example of the FET, a binary four-parameter logistic model (see Fig. 1a and Appendix) has been found suitable [27][28][29]. The EC xx concept does not rely on using the logistic model; it can be adapted to every other strictly monotone concentration-response model.…”

Section: Resultsmentioning

confidence: 99%

Optimal test design for binary response data: the example of the fish embryo toxicity test

Keddig¹,

Schubert²,

Wosniok

2015

Environ Sci Eur

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Background: The fish embryo toxicity test (FET) is an established method in toxicology research for quantifying the risk potential of environmental contaminations and other substances. The typical results of the method are the half maximal effective concentration (EC 50 ) or the no observed effect concentration (NOEC). However, from an environmental perspective, it is most important to safely identify the concentration of the substance effect which lies above the effect under control condition (spontaneous effect). The common FET is not optimal to detect ECs for small target effects. This paper shows how to optimize the efficiency and consequently the benefit of the FET for small effects using an adequate experimental design. The approach presented here can be carried over to all test systems generating binary (yes/no) outcomes. Results: The experimental design has three components in this context: determination of spontaneous response, sample size calculation, and dose allocation. A strategy for all three components is proposed from which a design is given including precision requirements and makes the most effective use of the experimental effort. This strategy amounts to expanding the usual FET guidelines of Organisation for Economic Co-operation and Development, German Institute for Standardization, or American Society for Testing and Materials by adding a planning step that adapts the test to the specific user's need. Conclusions: For the practical calculation of an adapted design, a newly developed software is presented as R package toxtestD. It provides a user-friendly way of developing an optimal experimental design for the FET without in-depth statistical knowledge. The programme is suited for all experimental problems involving a binary outcome and a continuous concentration.

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Acute toxicity of 353-nonylphenol and its metabolites for zebrafish embryos

Abstract: We have noticed that in our article published in the ESPR-March issue [Environ Sci Pollut Res 16(2):227−231 (2009)] the first name of the last author is wrong. "Andreas" Telscher has to be replaced by Markus Telscher.

Cited by 3 publications

References 0 publications

The impact of endocrine‐disrupting chemicals on oxidative stress and innate immune response in zebrafish embryos

The impact of endocrine‐disrupting chemicals on oxidative stress and innate immune response in zebrafish embryos

Microinjection into zebrafish embryos (Danio rerio) - a useful tool in aquatic toxicity testing?

Optimal test design for binary response data: the example of the fish embryo toxicity test

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